Cryopreservation of Cyrtopodium hatschbachii Pabst (Orchidaceae) immature seeds by encapsulation-dehydration

The aim of the present study was to investigate the efficiency of the encapsulation-dehydra- tion technique for cryopreservation of Cyrtopodium hastchbachii Pabst seeds. Immature seeds of this species were cryopreserved by an encapsulation-dehydration technique. Seeds of five immature pods, 120...

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Autores principales: Surenciski, Mauro Rodrigo, Flachsland, Eduardo Alberto, Terada, Graciela Raquel, Mroginski, Luis Amado, Rey, Hebe Yolanda
Formato: Artículo
Lenguaje:Inglés
Publicado: Tech Science Press 2024
Materias:
Cryoconservation
Liquid nitrogen
Calcium alginate
Activated charcoal
Sucrose
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/55354
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-55354
record_format dspace
spelling I48-R184-123456789-553542025-02-07T14:04:41Z Cryopreservation of Cyrtopodium hatschbachii Pabst (Orchidaceae) immature seeds by encapsulation-dehydration Surenciski, Mauro Rodrigo Flachsland, Eduardo Alberto Terada, Graciela Raquel Mroginski, Luis Amado Rey, Hebe Yolanda Cryoconservation Liquid nitrogen Calcium alginate Activated charcoal Sucrose The aim of the present study was to investigate the efficiency of the encapsulation-dehydra- tion technique for cryopreservation of Cyrtopodium hastchbachii Pabst seeds. Immature seeds of this species were cryopreserved by an encapsulation-dehydration technique. Seeds of five immature pods, 120 days after pollination, were encapsulated in 3% calcium alginate matrix and pretreated in liquid medium supplemented with 0.08 M sucrose (24 h), 0.15 M sucrose (24 h), 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h) in shaker at 60 rpm. Alginate beads were dehydrated 5 h in silicagel and immersed in liquid nitrogen for 12 h. Cryopreserved beads were thawed at 30°C for 1 min, rehydrated using the same liquid mediums [0.75 M sucrose (24 h), 0.5 M sucrose (24 h), 0.25 M sucrose (48 h) and 0.15 M sucrose (24 h)] and cultivated in half strength Murashige & Skoog medium (1962) with the addition of 2 g/L activated charcoal. Sixty four percent of seeds survived and developed into acclimatized plants after being cryopreserved. In this work, the encapsulation-dehydration technique was employed for first time in Cyrtopodium hatschbachii. 2024-09-18T15:27:52Z 2024-09-18T15:27:52Z 2012 Artículo Surenciski, Mauro Rodrigo, et al., 2012. Cryopreservation of Cyrtopodium hatschbachii Pabst (Orchidaceae) immature seeds by encapsulation-dehydration. Biocell. Henderson: Tech Science Press, vol. 36, no. 1, p. 31-36. E-ISSN 1667-5746. 0327-9545 http://repositorio.unne.edu.ar/handle/123456789/55354 eng https://doi.org/10.32604/biocell.2012.36.031 openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 31-36 application/pdf Tech Science Press Biocell, 2012, vol. 36, no. 1, p. 31-36.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Cryoconservation
Liquid nitrogen
Calcium alginate
Activated charcoal
Sucrose
spellingShingle Cryoconservation
Liquid nitrogen
Calcium alginate
Activated charcoal
Sucrose
Surenciski, Mauro Rodrigo
Flachsland, Eduardo Alberto
Terada, Graciela Raquel
Mroginski, Luis Amado
Rey, Hebe Yolanda
Cryopreservation of Cyrtopodium hatschbachii Pabst (Orchidaceae) immature seeds by encapsulation-dehydration
topic_facet Cryoconservation
Liquid nitrogen
Calcium alginate
Activated charcoal
Sucrose
description The aim of the present study was to investigate the efficiency of the encapsulation-dehydra- tion technique for cryopreservation of Cyrtopodium hastchbachii Pabst seeds. Immature seeds of this species were cryopreserved by an encapsulation-dehydration technique. Seeds of five immature pods, 120 days after pollination, were encapsulated in 3% calcium alginate matrix and pretreated in liquid medium supplemented with 0.08 M sucrose (24 h), 0.15 M sucrose (24 h), 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h) in shaker at 60 rpm. Alginate beads were dehydrated 5 h in silicagel and immersed in liquid nitrogen for 12 h. Cryopreserved beads were thawed at 30°C for 1 min, rehydrated using the same liquid mediums [0.75 M sucrose (24 h), 0.5 M sucrose (24 h), 0.25 M sucrose (48 h) and 0.15 M sucrose (24 h)] and cultivated in half strength Murashige & Skoog medium (1962) with the addition of 2 g/L activated charcoal. Sixty four percent of seeds survived and developed into acclimatized plants after being cryopreserved. In this work, the encapsulation-dehydration technique was employed for first time in Cyrtopodium hatschbachii.
format Artículo
author Surenciski, Mauro Rodrigo
Flachsland, Eduardo Alberto
Terada, Graciela Raquel
Mroginski, Luis Amado
Rey, Hebe Yolanda
author_facet Surenciski, Mauro Rodrigo
Flachsland, Eduardo Alberto
Terada, Graciela Raquel
Mroginski, Luis Amado
Rey, Hebe Yolanda
author_sort Surenciski, Mauro Rodrigo
title Cryopreservation of Cyrtopodium hatschbachii Pabst (Orchidaceae) immature seeds by encapsulation-dehydration
title_short Cryopreservation of Cyrtopodium hatschbachii Pabst (Orchidaceae) immature seeds by encapsulation-dehydration
title_full Cryopreservation of Cyrtopodium hatschbachii Pabst (Orchidaceae) immature seeds by encapsulation-dehydration
title_fullStr Cryopreservation of Cyrtopodium hatschbachii Pabst (Orchidaceae) immature seeds by encapsulation-dehydration
title_full_unstemmed Cryopreservation of Cyrtopodium hatschbachii Pabst (Orchidaceae) immature seeds by encapsulation-dehydration
title_sort cryopreservation of cyrtopodium hatschbachii pabst (orchidaceae) immature seeds by encapsulation-dehydration
publisher Tech Science Press
publishDate 2024
url http://repositorio.unne.edu.ar/handle/123456789/55354
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