Comparison of serological techniques for the diagnosis of equine infectious Anemia in an endemic area of Argentina
Equine Infectious Anemia is a transmissible viral disease present worldwide, caused by an RNA virus. Viral transmission is mainly mechanical through blood or its products most frequently by blood-sucking arthropods and iatrogenesis as well. OIE recommends Coggins Test as the diagnostic method of c...
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| Formato: | Artículo |
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Elsevier
2024
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| Acceso en línea: | http://repositorio.unne.edu.ar/handle/123456789/54616 |
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I48-R184-123456789-546162025-02-10T12:01:04Z Comparison of serological techniques for the diagnosis of equine infectious Anemia in an endemic area of Argentina Espasandin, Ana Gabriela Cipolini Galarza, María Fabiana Forletti, Agustina Díaz, Silvina Soto, Javier Martínez, Diana Elina Storani, Carlos Alberto Monzón, Nolly María Beltrame, Juan Ignacio Lucchesi, Esteban Soto, Pedro Coggins test Flurescence polarization assay cELISA Horses Equine Infectious Anemia is a transmissible viral disease present worldwide, caused by an RNA virus. Viral transmission is mainly mechanical through blood or its products most frequently by blood-sucking arthropods and iatrogenesis as well. OIE recommends Coggins Test as the diagnostic method of choice. Some ELISA tests detect antibodies earlier than the Coggins test, but may produce false-positive results. Currently, new techniques for EIA diagnosis are being developed, such as fluorescence polarization assay which is a simple method for measuring antigen-antibody interaction. The aim of this study was to assess cELISA and Fluorescence Polarization Assay performance for the serological diagnosis of EIA by comparing their results with those of the Coggins test. Tests were performed on 91 workhorses from an endemic zone in the northeast region of Argentina. From the total samples analyzed, 42 tested negative and 49 tested positive in the Coggins test. Same results were obtained using FPA. Using the cELISA, 41 negative results and 50 positive results were obtained. The Receiver Operating Characteristic analysis showed that FPA performance was excellent. Therefore FPA is proposed as an outstanding EIA diagnostic test to be validated in the near future by its simplicity, speed, and objective interpretation of results. 2024-08-01T17:21:09Z 2024-08-01T17:21:09Z 2021-05-10 Artículo Espasandin, Ana Gabriela, 2021. Comparison of serological techniques for the diagnosis of equine infectious Anemia in an endemic area of Argentina. En: Journal of Virological Methods, vol. 291, p.114101. ISSN 0166-0934. http://repositorio.unne.edu.ar/handle/123456789/54616 eng https://doi.org/10.1016/j.jviromet.2021.114101 restrictedAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 114101 application/pdf Elsevier Journal of Virological Methods, , may 2021, vol. 291, p. 114101 |
| institution |
Universidad Nacional del Nordeste |
| institution_str |
I-48 |
| repository_str |
R-184 |
| collection |
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) |
| language |
Inglés |
| topic |
Coggins test Flurescence polarization assay cELISA Horses |
| spellingShingle |
Coggins test Flurescence polarization assay cELISA Horses Espasandin, Ana Gabriela Cipolini Galarza, María Fabiana Forletti, Agustina Díaz, Silvina Soto, Javier Martínez, Diana Elina Storani, Carlos Alberto Monzón, Nolly María Beltrame, Juan Ignacio Lucchesi, Esteban Soto, Pedro Comparison of serological techniques for the diagnosis of equine infectious Anemia in an endemic area of Argentina |
| topic_facet |
Coggins test Flurescence polarization assay cELISA Horses |
| description |
Equine Infectious Anemia is a transmissible viral disease present worldwide, caused by an RNA virus. Viral
transmission is mainly mechanical through blood or its products most frequently by blood-sucking arthropods
and iatrogenesis as well. OIE recommends Coggins Test as the diagnostic method of choice. Some ELISA tests
detect antibodies earlier than the Coggins test, but may produce false-positive results. Currently, new techniques
for EIA diagnosis are being developed, such as fluorescence polarization assay which is a simple method for
measuring antigen-antibody interaction. The aim of this study was to assess cELISA and Fluorescence Polarization
Assay performance for the serological diagnosis of EIA by comparing their results with those of the
Coggins test. Tests were performed on 91 workhorses from an endemic zone in the northeast region of Argentina.
From the total samples analyzed, 42 tested negative and 49 tested positive in the Coggins test. Same results were
obtained using FPA. Using the cELISA, 41 negative results and 50 positive results were obtained. The Receiver
Operating Characteristic analysis showed that FPA performance was excellent. Therefore FPA is proposed as an
outstanding EIA diagnostic test to be validated in the near future by its simplicity, speed, and objective interpretation
of results. |
| format |
Artículo |
| author |
Espasandin, Ana Gabriela Cipolini Galarza, María Fabiana Forletti, Agustina Díaz, Silvina Soto, Javier Martínez, Diana Elina Storani, Carlos Alberto Monzón, Nolly María Beltrame, Juan Ignacio Lucchesi, Esteban Soto, Pedro |
| author_facet |
Espasandin, Ana Gabriela Cipolini Galarza, María Fabiana Forletti, Agustina Díaz, Silvina Soto, Javier Martínez, Diana Elina Storani, Carlos Alberto Monzón, Nolly María Beltrame, Juan Ignacio Lucchesi, Esteban Soto, Pedro |
| author_sort |
Espasandin, Ana Gabriela |
| title |
Comparison of serological techniques for the diagnosis of equine infectious Anemia in an endemic area of Argentina |
| title_short |
Comparison of serological techniques for the diagnosis of equine infectious Anemia in an endemic area of Argentina |
| title_full |
Comparison of serological techniques for the diagnosis of equine infectious Anemia in an endemic area of Argentina |
| title_fullStr |
Comparison of serological techniques for the diagnosis of equine infectious Anemia in an endemic area of Argentina |
| title_full_unstemmed |
Comparison of serological techniques for the diagnosis of equine infectious Anemia in an endemic area of Argentina |
| title_sort |
comparison of serological techniques for the diagnosis of equine infectious anemia in an endemic area of argentina |
| publisher |
Elsevier |
| publishDate |
2024 |
| url |
http://repositorio.unne.edu.ar/handle/123456789/54616 |
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