Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death

Hydroxy fatty acids are known to cause cell cycle arrest and apoptosis. The best studied of them, 9-hydroxystearic acid (9-HSA), induces apoptosis in cell lines by acting through mechanisms involving di erent targets. Using mass spectrometry-based lipidomic approaches, we show in this study that...

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Autores principales: Rodríguez, Juan Pablo, Guijas, Carlos, Astudillo, Alma María, Rubio, Julio Miguel, Balboa García, María Ángeles, Balsinde, Jesús
Formato: Artículo
Lenguaje:Inglés
Publicado: MDPI 2024
Materias:
Colorectal cancer
Hydroxystearic acid
Fatty acid esters of hydroxy fatty acids
Apoptosis
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/54383
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-54383
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spelling I48-R184-123456789-543832024-12-16T11:36:22Z Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death Rodríguez, Juan Pablo Guijas, Carlos Astudillo, Alma María Rubio, Julio Miguel Balboa García, María Ángeles Balsinde, Jesús Colorectal cancer Hydroxystearic acid Fatty acid esters of hydroxy fatty acids Apoptosis Hydroxy fatty acids are known to cause cell cycle arrest and apoptosis. The best studied of them, 9-hydroxystearic acid (9-HSA), induces apoptosis in cell lines by acting through mechanisms involving di erent targets. Using mass spectrometry-based lipidomic approaches, we show in this study that 9-HSA levels in human colorectal tumors are diminished when compared with normal adjacent tissue. Since this decrease could be compatible with an escape mechanism of tumors from 9-HSA-induced apoptosis, we investigated di erent features of the utilization of this hydroxyfatty acid in colon. We show that in colorectal tumors and related cell lines such as HT-29 and HCT-116, 9-HSA is the only hydroxyfatty acid constituent of branched fatty acid esters of hydroxyfatty acids (FAHFA), a novel family of lipids with anti-inflammatory properties. Importantly, FAHFA levels in tumors are elevated compared with normal tissue and, unlike 9-HSA, they do not induce apoptosis of colorectal cell lines over a wide range of concentrations. Further, the addition of 9-HSA to colon cancer cell lines augments the synthesis of di erent FAHFA before the cells commit to apoptosis, suggesting that FAHFA formation may function as a bu er system that sequesters the hydroxyacid into an inactive form, thereby restricting apoptosis. 2024-06-25T16:50:09Z 2024-06-25T16:50:09Z 2019-04-12 Artículo Rodríguez Juan Pablo, et al., 2019. Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death. Cánceres. Basilea: Multidisciplinary Digital Publishing Institute vol.11, no. 4 p.1-15. E-ISSN 2072-6694. http://repositorio.unne.edu.ar/handle/123456789/54383 eng https://doi.org/10.3390/cancers11040524 https://www.mdpi.com/2072-6694/11/4/524 openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 1-15 application/pdf MDPI Cánceres, 2019, vol.11, no. 4 p.1-15.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Colorectal cancer
Hydroxystearic acid
Fatty acid esters of hydroxy fatty acids
Apoptosis
spellingShingle Colorectal cancer
Hydroxystearic acid
Fatty acid esters of hydroxy fatty acids
Apoptosis
Rodríguez, Juan Pablo
Guijas, Carlos
Astudillo, Alma María
Rubio, Julio Miguel
Balboa García, María Ángeles
Balsinde, Jesús
Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death
topic_facet Colorectal cancer
Hydroxystearic acid
Fatty acid esters of hydroxy fatty acids
Apoptosis
description Hydroxy fatty acids are known to cause cell cycle arrest and apoptosis. The best studied of them, 9-hydroxystearic acid (9-HSA), induces apoptosis in cell lines by acting through mechanisms involving di erent targets. Using mass spectrometry-based lipidomic approaches, we show in this study that 9-HSA levels in human colorectal tumors are diminished when compared with normal adjacent tissue. Since this decrease could be compatible with an escape mechanism of tumors from 9-HSA-induced apoptosis, we investigated di erent features of the utilization of this hydroxyfatty acid in colon. We show that in colorectal tumors and related cell lines such as HT-29 and HCT-116, 9-HSA is the only hydroxyfatty acid constituent of branched fatty acid esters of hydroxyfatty acids (FAHFA), a novel family of lipids with anti-inflammatory properties. Importantly, FAHFA levels in tumors are elevated compared with normal tissue and, unlike 9-HSA, they do not induce apoptosis of colorectal cell lines over a wide range of concentrations. Further, the addition of 9-HSA to colon cancer cell lines augments the synthesis of di erent FAHFA before the cells commit to apoptosis, suggesting that FAHFA formation may function as a bu er system that sequesters the hydroxyacid into an inactive form, thereby restricting apoptosis.
format Artículo
author Rodríguez, Juan Pablo
Guijas, Carlos
Astudillo, Alma María
Rubio, Julio Miguel
Balboa García, María Ángeles
Balsinde, Jesús
author_facet Rodríguez, Juan Pablo
Guijas, Carlos
Astudillo, Alma María
Rubio, Julio Miguel
Balboa García, María Ángeles
Balsinde, Jesús
author_sort Rodríguez, Juan Pablo
title Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death
title_short Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death
title_full Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death
title_fullStr Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death
title_full_unstemmed Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death
title_sort sequestration of 9-hydroxystearic acid in fahfa (fatty acid esters of hydroxy fatty acids) as a protective mechanism for colon carcinoma cells to avoid apoptotic cell death
publisher MDPI
publishDate 2024
url http://repositorio.unne.edu.ar/handle/123456789/54383
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