Heterochromatin evolution in Arachis investigated through genome‑wide analysis of repetitive DNA
Main conclusion The most conspicuous diference among chromosomes and genomes in Arachis species, the patterns of heterochromatin, was mainly modeled by diferential amplifcation of diferent members of one superfamily of satellite DNAs. Divergence in repetitive DNA is a primary driving force for...
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| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer Nature
2024
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| Materias: | |
| Acceso en línea: | http://repositorio.unne.edu.ar/handle/123456789/54375 |
| Aporte de: |
| Sumario: | Main conclusion The most conspicuous diference among chromosomes and genomes in Arachis species, the patterns
of heterochromatin, was mainly modeled by diferential amplifcation of diferent members of one superfamily of
satellite DNAs.
Divergence in repetitive DNA is a primary driving force for genome and chromosome evolution. Section Arachis is karyo typically diverse and has six diferent genomes. Arachis glandulifera (D genome) has the most asymmetric karyotype and
the highest reproductive isolation compared to the well-known A and B genome species. These features make A. glandulifera
an interesting model species for studying the main repetitive components that accompanied the genome and chromosome
diversifcation in the section. Here, we performed a genome-wide analysis of repetitive sequences in A. glandulifera and
investigated the chromosome distribution of the identifed satellite DNA sequences (satDNAs). LTR retroelements, mainly
the Ty3-gypsy families “Fidel/Feral” and “Pipoka/Pipa”, were the most represented. Comparative analyses with the A and
B genomes showed that many of the previously described transposable elements (TEs) were diferently represented in the
D genome, and that this variation accompanied changes in DNA content. In addition, four major satDNAs were character ized. Agla_CL8sat was the major component of pericentromeric heterochromatin, while Agla_CL39sat, Agla_CL69sat, and
Agla_CL122sat were found in heterochromatic and/or euchromatic regions. Even though Agla_CL8sat belong to a diferent
family than that of the major satDNA (ATR-2) found in the heterochromatin of the A, K, and F genomes, both satDNAs are
members of the same superfamily. This fnding suggests that closely related satDNAs of an ancestral library were diferen tially amplifed leading to the major changes in the heterochromatin patterns that accompanied the karyotype and genome
diferentiation in Arachis |
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