A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation

Water buffalo (Bubalus bubalis) and domestic cattle (Bos taurus) are closely related species. However, embryo transfer interspecies has been attempted without any success. The failure in hybrid embryo-implantation is associated with the glycocode in the maternal-fetal interface. Glycosylation patt...

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Autores principales: Caspe, Sergio Gastón, Konrad, José Luis, Moore, Dadin Prando, Sala, Juan Manuel, Lischinsky, Lilian, Campero, Carlos Manuel, Barbeito, Claudio Gustavo
Formato: Artículo
Lenguaje:Inglés
Publicado: Instituto de Histología y Embriología 2024
Materias:
Lectin-histochemistry
Water buffalo
Placenta
Glycosylation pattern
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/53157
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-53157
record_format dspace
spelling I48-R184-123456789-531572024-10-23T10:49:05Z A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation Caspe, Sergio Gastón Konrad, José Luis Moore, Dadin Prando Sala, Juan Manuel Lischinsky, Lilian Campero, Carlos Manuel Barbeito, Claudio Gustavo Lectin-histochemistry Water buffalo Placenta Glycosylation pattern Water buffalo (Bubalus bubalis) and domestic cattle (Bos taurus) are closely related species. However, embryo transfer interspecies has been attempted without any success. The failure in hybrid embryo-implantation is associated with the glycocode in the maternal-fetal interface. Glycosylation patterns have been studied in different species of ruminants; however, in B. bubalis, only the binucleated cells (BNC) have been analyzed. This glycocode is essential for a successful embryo-implantation and can be defined by Lectin-Histochemistry (LHC). The aim of this study is to compare the glycosylation pattern of placenta and uterus in water buffaloes and cattle by LHC. Tissue samples of placenta and uterus from pregnant Mediterranean female water buffaloes (Buf1) and Angus cows (Bov1) were analyzed. All animals were euthanized at 98 days of gestation. LHC was carried out using twelve lectins (Con A, LCA, PSA, sWGA, PHA-e, SBA, UEA-1, WGA, RCA-1, PNA, DBA, BSA-1). The intensity of lectin binding was semi- quantitatively scored using a scale of 0 (negative) to 3 (strongly positive). Difference between species was found in trophoblast layer by PSA, SWGA, PNA and BSA-1, in BNC, and in the mononuclear cells by LCA, PSA, PHA-e, DBA, BSA-1, PNA. In utero, differences in the apical cellular membrane and the secretion of glands were identified by DBA and RCA-1, and in the cytoplasm of those glandular epithelial cells by PHA-e, BSA-1, WGA, and SBA. In both species, BNC presented a strong positive reaction with DBA and SBA, a moderate response by LCA, PHA-e, BSA-1 and PNA lectin, and a low reaction by PSA, UEA-1, SWGA, WGA, Con A and RCA-1. The results found in this study suggest that although both species are closely related, glycosylation patterns in the placenta and uterus are different, thus providing a possible reason for embryo transfer not being possible between these species. 2024-03-20T12:06:36Z 2024-03-20T12:06:36Z 2021 Artículo Caspe, Sergio Gastón, et al., 2021. A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation. Biocell. Mendoza: Instituto de Histología y Embriología, vol. 45, no. 2, p. 331-337. E-ISSN 1667-5746. 0327-9545 http://repositorio.unne.edu.ar/handle/123456789/53157 eng openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 331-337 application/pdf Instituto de Histología y Embriología Biocell, 2021, vol. 45, no. 2, p. 331-337.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Lectin-histochemistry
Water buffalo
Placenta
Glycosylation pattern
spellingShingle Lectin-histochemistry
Water buffalo
Placenta
Glycosylation pattern
Caspe, Sergio Gastón
Konrad, José Luis
Moore, Dadin Prando
Sala, Juan Manuel
Lischinsky, Lilian
Campero, Carlos Manuel
Barbeito, Claudio Gustavo
A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation
topic_facet Lectin-histochemistry
Water buffalo
Placenta
Glycosylation pattern
description Water buffalo (Bubalus bubalis) and domestic cattle (Bos taurus) are closely related species. However, embryo transfer interspecies has been attempted without any success. The failure in hybrid embryo-implantation is associated with the glycocode in the maternal-fetal interface. Glycosylation patterns have been studied in different species of ruminants; however, in B. bubalis, only the binucleated cells (BNC) have been analyzed. This glycocode is essential for a successful embryo-implantation and can be defined by Lectin-Histochemistry (LHC). The aim of this study is to compare the glycosylation pattern of placenta and uterus in water buffaloes and cattle by LHC. Tissue samples of placenta and uterus from pregnant Mediterranean female water buffaloes (Buf1) and Angus cows (Bov1) were analyzed. All animals were euthanized at 98 days of gestation. LHC was carried out using twelve lectins (Con A, LCA, PSA, sWGA, PHA-e, SBA, UEA-1, WGA, RCA-1, PNA, DBA, BSA-1). The intensity of lectin binding was semi- quantitatively scored using a scale of 0 (negative) to 3 (strongly positive). Difference between species was found in trophoblast layer by PSA, SWGA, PNA and BSA-1, in BNC, and in the mononuclear cells by LCA, PSA, PHA-e, DBA, BSA-1, PNA. In utero, differences in the apical cellular membrane and the secretion of glands were identified by DBA and RCA-1, and in the cytoplasm of those glandular epithelial cells by PHA-e, BSA-1, WGA, and SBA. In both species, BNC presented a strong positive reaction with DBA and SBA, a moderate response by LCA, PHA-e, BSA-1 and PNA lectin, and a low reaction by PSA, UEA-1, SWGA, WGA, Con A and RCA-1. The results found in this study suggest that although both species are closely related, glycosylation patterns in the placenta and uterus are different, thus providing a possible reason for embryo transfer not being possible between these species.
format Artículo
author Caspe, Sergio Gastón
Konrad, José Luis
Moore, Dadin Prando
Sala, Juan Manuel
Lischinsky, Lilian
Campero, Carlos Manuel
Barbeito, Claudio Gustavo
author_facet Caspe, Sergio Gastón
Konrad, José Luis
Moore, Dadin Prando
Sala, Juan Manuel
Lischinsky, Lilian
Campero, Carlos Manuel
Barbeito, Claudio Gustavo
author_sort Caspe, Sergio Gastón
title A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation
title_short A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation
title_full A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation
title_fullStr A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation
title_full_unstemmed A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation
title_sort comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation
publisher Instituto de Histología y Embriología
publishDate 2024
url http://repositorio.unne.edu.ar/handle/123456789/53157
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