Characterization and cytotoxic activity on glial cells of alkaloid-enriched extracts from pods of the plants prosopis flexuosa and prosopis Nigra (Fabaceae)

Introduction: Prosopis spp. pods have shown to be a potential source of protein and energy in livestock. However, prolonged ingestion of some of these species produces neurological symptoms in ruminants. Objective: In the present study, the alkaloid content and the in vitro neurotoxic activity of...

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Autores principales:	Cholich, Luciana Andrea, Pistán, María Elena, Torres, Ana María, Ortega, Hugo Héctor, Gardner, Dale R., Bustillo, Soledad
Formato:	Artículo
Lenguaje:	Inglés
Publicado:	Universidad de Costa Rica 2024
Materias:	Algarrobos Cytotoxicity Piperidine alkaloids Prosopis species
Acceso en línea:	http://repositorio.unne.edu.ar/handle/123456789/53153
Aporte de:	RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste

id	I48-R184-123456789-53153
record_format	dspace
spelling	I48-R184-123456789-531532025-03-06T10:59:15Z Characterization and cytotoxic activity on glial cells of alkaloid-enriched extracts from pods of the plants prosopis flexuosa and prosopis Nigra (Fabaceae) Cholich, Luciana Andrea Pistán, María Elena Torres, Ana María Ortega, Hugo Héctor Gardner, Dale R. Bustillo, Soledad Algarrobos Cytotoxicity Piperidine alkaloids Prosopis species Introduction: Prosopis spp. pods have shown to be a potential source of protein and energy in livestock. However, prolonged ingestion of some of these species produces neurological symptoms in ruminants. Objective: In the present study, the alkaloid content and the in vitro neurotoxic activity of alkaloid enrichedextracts from P. flexuosa and P. nigra pods were determined in order to elucidate the mechanism of animal poisoning caused by these species. Methods: The main alkaloids present in both extracts were analysed by high performance liquid chromatography-high resolution mass spectrometry (HPLC-HRMS). The cytotoxic activity of Prosopis alkaloid enriched-extracts in primary mixed glial cell culture was assessed by phase contrast microscopy and using neutral red, and lactate dehydrogenase (LDH) activity assays. Results: Juliprosine and juliprosopine were identified in P. flexuosa pods, while the absence of these alkaloids in P. nigra was confirmed. Both extracts (5-30 μg/mL) induced in a dose dependent manner, morphological alterations, such as swelling, enlargement and detachment from the culture surface. Consistent with this, decrease in cell viability and release of LDH 48 hours after exposure, revealed that P. flexuosa pods was significantly more cytotoxic than P. nigra. Conclusions: In P. flexuosa pods, juliprosine and juliprosopine alkaloids were identified for the first time. Moreover, the present study suggests that the cytotoxic effect displayed by both extracts is due to its alkaloid content. However, the presence of piperidine alkaloids in P. flexuosa could explain the greater cytotoxicity on glial cells with respect to P. nigra that was not shown to contain these alkaloids 2024-03-20T10:45:17Z 2024-03-20T10:45:17Z 2021 Artículo Cholich, Luciana Andrea., et. al., 2021. Characterization and cytotoxic activity on glial cells of alkaloid-enriched extracts from pods of the plants prosopis flexuosa and prosopis Nigra (Fabaceae). Revista de Biología Tropical. San José: Universidad de Costa Rica, vol. 69, no. 1, p. 197-2060. E-ISSN 2215-2075. 0034-7744 http://repositorio.unne.edu.ar/handle/123456789/53153 eng openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 197-206 application/pdf Universidad de Costa Rica Revista de Biología Tropical, 2021, vol. 69, no. 1, p. 197-206.
institution	Universidad Nacional del Nordeste
institution_str	I-48
repository_str	R-184
collection	RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language	Inglés
topic	Algarrobos Cytotoxicity Piperidine alkaloids Prosopis species
spellingShingle	Algarrobos Cytotoxicity Piperidine alkaloids Prosopis species Cholich, Luciana Andrea Pistán, María Elena Torres, Ana María Ortega, Hugo Héctor Gardner, Dale R. Bustillo, Soledad Characterization and cytotoxic activity on glial cells of alkaloid-enriched extracts from pods of the plants prosopis flexuosa and prosopis Nigra (Fabaceae)
topic_facet	Algarrobos Cytotoxicity Piperidine alkaloids Prosopis species
description	Introduction: Prosopis spp. pods have shown to be a potential source of protein and energy in livestock. However, prolonged ingestion of some of these species produces neurological symptoms in ruminants. Objective: In the present study, the alkaloid content and the in vitro neurotoxic activity of alkaloid enrichedextracts from P. flexuosa and P. nigra pods were determined in order to elucidate the mechanism of animal poisoning caused by these species. Methods: The main alkaloids present in both extracts were analysed by high performance liquid chromatography-high resolution mass spectrometry (HPLC-HRMS). The cytotoxic activity of Prosopis alkaloid enriched-extracts in primary mixed glial cell culture was assessed by phase contrast microscopy and using neutral red, and lactate dehydrogenase (LDH) activity assays. Results: Juliprosine and juliprosopine were identified in P. flexuosa pods, while the absence of these alkaloids in P. nigra was confirmed. Both extracts (5-30 μg/mL) induced in a dose dependent manner, morphological alterations, such as swelling, enlargement and detachment from the culture surface. Consistent with this, decrease in cell viability and release of LDH 48 hours after exposure, revealed that P. flexuosa pods was significantly more cytotoxic than P. nigra. Conclusions: In P. flexuosa pods, juliprosine and juliprosopine alkaloids were identified for the first time. Moreover, the present study suggests that the cytotoxic effect displayed by both extracts is due to its alkaloid content. However, the presence of piperidine alkaloids in P. flexuosa could explain the greater cytotoxicity on glial cells with respect to P. nigra that was not shown to contain these alkaloids
format	Artículo
author	Cholich, Luciana Andrea Pistán, María Elena Torres, Ana María Ortega, Hugo Héctor Gardner, Dale R. Bustillo, Soledad
author_facet	Cholich, Luciana Andrea Pistán, María Elena Torres, Ana María Ortega, Hugo Héctor Gardner, Dale R. Bustillo, Soledad
author_sort	Cholich, Luciana Andrea
title	Characterization and cytotoxic activity on glial cells of alkaloid-enriched extracts from pods of the plants prosopis flexuosa and prosopis Nigra (Fabaceae)
title_short	Characterization and cytotoxic activity on glial cells of alkaloid-enriched extracts from pods of the plants prosopis flexuosa and prosopis Nigra (Fabaceae)
title_full	Characterization and cytotoxic activity on glial cells of alkaloid-enriched extracts from pods of the plants prosopis flexuosa and prosopis Nigra (Fabaceae)
title_fullStr	Characterization and cytotoxic activity on glial cells of alkaloid-enriched extracts from pods of the plants prosopis flexuosa and prosopis Nigra (Fabaceae)
title_full_unstemmed	Characterization and cytotoxic activity on glial cells of alkaloid-enriched extracts from pods of the plants prosopis flexuosa and prosopis Nigra (Fabaceae)
title_sort	characterization and cytotoxic activity on glial cells of alkaloid-enriched extracts from pods of the plants prosopis flexuosa and prosopis nigra (fabaceae)
publisher	Universidad de Costa Rica
publishDate	2024
url	http://repositorio.unne.edu.ar/handle/123456789/53153
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Characterization and cytotoxic activity on glial cells of alkaloid-enriched extracts from pods of the plants prosopis flexuosa and prosopis Nigra (Fabaceae)

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