In vitro conservation of apical meristem-tip of Melia azedarach L. (Meliaceae) under slow-growth conditions (with 1 table & 2 figures)

Studies on in vitro storage of Melia azedarach –“paradise tree”- under slow-growth conditions were carried out to develop an efficient protocol for the con- servation of the genetic diversity of the crop. The response to different growth re- tardation treatments was examined using clone E. In v...

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Autores principales: Scocchi, Adriana M., Mroginski, Luis Amado
Formato: Artículo
Lenguaje:Inglés
Publicado: Fundación Romulo Raggio 2024
Materias:
Conservation
Germplasm
In vitro culture
Melia azedarach
Meristem
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/53131
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-53131
record_format dspace
spelling I48-R184-123456789-531312024-10-03T12:57:28Z In vitro conservation of apical meristem-tip of Melia azedarach L. (Meliaceae) under slow-growth conditions (with 1 table & 2 figures) Scocchi, Adriana M. Mroginski, Luis Amado Conservation Germplasm In vitro culture Melia azedarach Meristem Studies on in vitro storage of Melia azedarach –“paradise tree”- under slow-growth conditions were carried out to develop an efficient protocol for the con- servation of the genetic diversity of the crop. The response to different growth re- tardation treatments was examined using clone E. In vitro cultures of apical mer- istem-tips (0.2-0.3 mm in length) could be effectively maintained for 12 months at 4oC on 1⁄4MS basal medium supplemented with 0.5μM BA. Shoot proliferation was observed on 67% of the recovered apical meristem-tips, after 12 months at 4oC. Rooting of the regenerated shoots was achieved by reculturing them on MS + 17.5 μM IBA, during 4 days and subsequently transferred to MS medium lacking growth- regulators for 30 days. Complete plants were successfully transferred to the green- house. 2024-03-13T12:48:09Z 2024-03-13T12:48:09Z 2004 Artículo Scocchi, Adriana M. y Mroginski, Luis Amado, 2004. In vitro conservation of apical meristem-tip of Melia azedarach L. (Meliaceae) under slow-growth conditions (with 1 table & 2 figures). Phyton International Journal of Experimental Botany. Buenos Aires: Fundación Romulo Raggio, vol. 73, p. 137-143. E-ISSN 1851-5657. 0031-9457 http://repositorio.unne.edu.ar/handle/123456789/53131 eng https://www.scielo.org.ar/pdf/phyton/v73/v73a17.pdf openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 137-143 application/pdf Fundación Romulo Raggio Phyton International Journal of Experimental Botany, 2004, vol. 73, p. 137-143.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Conservation
Germplasm
In vitro culture
Melia azedarach
Meristem
spellingShingle Conservation
Germplasm
In vitro culture
Melia azedarach
Meristem
Scocchi, Adriana M.
Mroginski, Luis Amado
In vitro conservation of apical meristem-tip of Melia azedarach L. (Meliaceae) under slow-growth conditions (with 1 table & 2 figures)
topic_facet Conservation
Germplasm
In vitro culture
Melia azedarach
Meristem
description Studies on in vitro storage of Melia azedarach –“paradise tree”- under slow-growth conditions were carried out to develop an efficient protocol for the con- servation of the genetic diversity of the crop. The response to different growth re- tardation treatments was examined using clone E. In vitro cultures of apical mer- istem-tips (0.2-0.3 mm in length) could be effectively maintained for 12 months at 4oC on 1⁄4MS basal medium supplemented with 0.5μM BA. Shoot proliferation was observed on 67% of the recovered apical meristem-tips, after 12 months at 4oC. Rooting of the regenerated shoots was achieved by reculturing them on MS + 17.5 μM IBA, during 4 days and subsequently transferred to MS medium lacking growth- regulators for 30 days. Complete plants were successfully transferred to the green- house.
format Artículo
author Scocchi, Adriana M.
Mroginski, Luis Amado
author_facet Scocchi, Adriana M.
Mroginski, Luis Amado
author_sort Scocchi, Adriana M.
title In vitro conservation of apical meristem-tip of Melia azedarach L. (Meliaceae) under slow-growth conditions (with 1 table & 2 figures)
title_short In vitro conservation of apical meristem-tip of Melia azedarach L. (Meliaceae) under slow-growth conditions (with 1 table & 2 figures)
title_full In vitro conservation of apical meristem-tip of Melia azedarach L. (Meliaceae) under slow-growth conditions (with 1 table & 2 figures)
title_fullStr In vitro conservation of apical meristem-tip of Melia azedarach L. (Meliaceae) under slow-growth conditions (with 1 table & 2 figures)
title_full_unstemmed In vitro conservation of apical meristem-tip of Melia azedarach L. (Meliaceae) under slow-growth conditions (with 1 table & 2 figures)
title_sort in vitro conservation of apical meristem-tip of melia azedarach l. (meliaceae) under slow-growth conditions (with 1 table & 2 figures)
publisher Fundación Romulo Raggio
publishDate 2024
url http://repositorio.unne.edu.ar/handle/123456789/53131
work_keys_str_mv AT scocchiadrianam invitroconservationofapicalmeristemtipofmeliaazedarachlmeliaceaeunderslowgrowthconditionswith1table2figures
AT mroginskiluisamado invitroconservationofapicalmeristemtipofmeliaazedarachlmeliaceaeunderslowgrowthconditionswith1table2figures
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