Gametogenesis and reproductive dynamics of Scinax acuminatus (Anura: Hylidae) : morphological, histological and immunohistochemical analysis

To characterize the reproductive biology of Scinax acuminatus and contribute to the natural history of this species, the morphology of the reproductive system of males and females was analyzed at anatomical, histological and immunohistochemical levels. The individuals were collected fortnightly b...

Descripción completa

Detalles Bibliográficos
Autores principales: Olea, Gabriela Beatriz, Cheij, Esteban Omar, Cuzziol Boccioni, Ana Paula, Rodríguez, Florencia Evelyn, Céspedez, Jorge Abel, Lombardo, Daniel Marcelo
Formato: Artículo
Lenguaje:Inglés
Publicado: Academia Brasileira de Ciencias 2024
Materias:
Amphibians
Ovaries
Reproduction
Testicles
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/53084
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-53084
record_format dspace
spelling I48-R184-123456789-530842025-03-06T10:57:45Z Gametogenesis and reproductive dynamics of Scinax acuminatus (Anura: Hylidae) : morphological, histological and immunohistochemical analysis Olea, Gabriela Beatriz Cheij, Esteban Omar Cuzziol Boccioni, Ana Paula Rodríguez, Florencia Evelyn Céspedez, Jorge Abel Lombardo, Daniel Marcelo Amphibians Ovaries Reproduction Testicles To characterize the reproductive biology of Scinax acuminatus and contribute to the natural history of this species, the morphology of the reproductive system of males and females was analyzed at anatomical, histological and immunohistochemical levels. The individuals were collected fortnightly between August and December (2016) and January to December (2018). The anatomy of the reproductive system was analyzed in a stereoscopic microscope, and histological preparations staining with hematoxylin-eosin and Masson’s trichromic, PAS and Coomassie Blue was performed as well. To characterize the meiotic-active cells in the testes, immunostaining with the PCNA proliferation protein was performed. There were found females with ovaries with oocytes in different stages of maturity and post-ovulatory females. Males presented continuous spermatogenesis, which could be confirmed by the immunostaining of PCNA in spermatogonia during the cycle. The results of this work serve as a basis for the characterization of the reproductive cycle in S. acuminatus and provide background information on the analysis of spermatogenic activity by IHQ from the study of the immunodetection of the PCNA cell proliferation protein. Future studies will focus on the evaluation of cell death processes during the reproductive cycle in the studied species to compare with those obtained in terms of cell proliferation. 2024-03-01T15:14:26Z 2024-03-01T15:14:26Z 2021 Artículo Olea, Gabriela Beatriz, et al., 2021. Gametogenesis and reproductive dynamics of Scinax acuminatus (Anura: Hylidae) : morphological, histological and immunohistochemical analysis. Anais da Academia Brasileira de Ciencias. Rio de Janeiro: Academia Brasileira de Ciencias, vol. 93, no. 2, p. 1-11. E-ISSN 1678-2690. 0001-3765 http://repositorio.unne.edu.ar/handle/123456789/53084 eng openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 1-11 application/pdf Academia Brasileira de Ciencias Anais da Academia Brasileira de Ciencias, 2021, vol. 93, no. 2, p. 1-11.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Amphibians
Ovaries
Reproduction
Testicles
spellingShingle Amphibians
Ovaries
Reproduction
Testicles
Olea, Gabriela Beatriz
Cheij, Esteban Omar
Cuzziol Boccioni, Ana Paula
Rodríguez, Florencia Evelyn
Céspedez, Jorge Abel
Lombardo, Daniel Marcelo
Gametogenesis and reproductive dynamics of Scinax acuminatus (Anura: Hylidae) : morphological, histological and immunohistochemical analysis
topic_facet Amphibians
Ovaries
Reproduction
Testicles
description To characterize the reproductive biology of Scinax acuminatus and contribute to the natural history of this species, the morphology of the reproductive system of males and females was analyzed at anatomical, histological and immunohistochemical levels. The individuals were collected fortnightly between August and December (2016) and January to December (2018). The anatomy of the reproductive system was analyzed in a stereoscopic microscope, and histological preparations staining with hematoxylin-eosin and Masson’s trichromic, PAS and Coomassie Blue was performed as well. To characterize the meiotic-active cells in the testes, immunostaining with the PCNA proliferation protein was performed. There were found females with ovaries with oocytes in different stages of maturity and post-ovulatory females. Males presented continuous spermatogenesis, which could be confirmed by the immunostaining of PCNA in spermatogonia during the cycle. The results of this work serve as a basis for the characterization of the reproductive cycle in S. acuminatus and provide background information on the analysis of spermatogenic activity by IHQ from the study of the immunodetection of the PCNA cell proliferation protein. Future studies will focus on the evaluation of cell death processes during the reproductive cycle in the studied species to compare with those obtained in terms of cell proliferation.
format Artículo
author Olea, Gabriela Beatriz
Cheij, Esteban Omar
Cuzziol Boccioni, Ana Paula
Rodríguez, Florencia Evelyn
Céspedez, Jorge Abel
Lombardo, Daniel Marcelo
author_facet Olea, Gabriela Beatriz
Cheij, Esteban Omar
Cuzziol Boccioni, Ana Paula
Rodríguez, Florencia Evelyn
Céspedez, Jorge Abel
Lombardo, Daniel Marcelo
author_sort Olea, Gabriela Beatriz
title Gametogenesis and reproductive dynamics of Scinax acuminatus (Anura: Hylidae) : morphological, histological and immunohistochemical analysis
title_short Gametogenesis and reproductive dynamics of Scinax acuminatus (Anura: Hylidae) : morphological, histological and immunohistochemical analysis
title_full Gametogenesis and reproductive dynamics of Scinax acuminatus (Anura: Hylidae) : morphological, histological and immunohistochemical analysis
title_fullStr Gametogenesis and reproductive dynamics of Scinax acuminatus (Anura: Hylidae) : morphological, histological and immunohistochemical analysis
title_full_unstemmed Gametogenesis and reproductive dynamics of Scinax acuminatus (Anura: Hylidae) : morphological, histological and immunohistochemical analysis
title_sort gametogenesis and reproductive dynamics of scinax acuminatus (anura: hylidae) : morphological, histological and immunohistochemical analysis
publisher Academia Brasileira de Ciencias
publishDate 2024
url http://repositorio.unne.edu.ar/handle/123456789/53084
work_keys_str_mv AT oleagabrielabeatriz gametogenesisandreproductivedynamicsofscinaxacuminatusanurahylidaemorphologicalhistologicalandimmunohistochemicalanalysis
AT cheijestebanomar gametogenesisandreproductivedynamicsofscinaxacuminatusanurahylidaemorphologicalhistologicalandimmunohistochemicalanalysis
AT cuzziolboccionianapaula gametogenesisandreproductivedynamicsofscinaxacuminatusanurahylidaemorphologicalhistologicalandimmunohistochemicalanalysis
AT rodriguezflorenciaevelyn gametogenesisandreproductivedynamicsofscinaxacuminatusanurahylidaemorphologicalhistologicalandimmunohistochemicalanalysis
AT cespedezjorgeabel gametogenesisandreproductivedynamicsofscinaxacuminatusanurahylidaemorphologicalhistologicalandimmunohistochemicalanalysis
AT lombardodanielmarcelo gametogenesisandreproductivedynamicsofscinaxacuminatusanurahylidaemorphologicalhistologicalandimmunohistochemicalanalysis
_version_ 1832343715380723712

Ejemplares similares