Localization and distribution of CCK-8-, NPY-, Leu-ENK-, and Ghrelin- in the digestive tract of Prochilodus lineatus (Valenciennes, 1836)

This study describes the histological characteristics and distribution of gastrointestinal tract endocrine cells (ECs) of Prochilodus lineatus (detritivorous fish) using immunohistochemical procedures. The digestive tract of P. lineatus was divided into seven portions: stomach (cardial and pylori...

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Autores principales: Barrios, Carlos Eduardo, Santinón, Juan José, Domitrovic, Hugo Alberto, Sánchez, Sebastián, Henández, David Roque
Formato: Artículo
Lenguaje:Inglés
Publicado: Academia Brasileira de Ciencias 2024
Materias:
Detritivorous fish
Sábalo
Endocrine cells
Immunohistochemistry
Neuropeptide
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/53054
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-53054
record_format dspace
spelling I48-R184-123456789-530542024-10-23T10:49:38Z Localization and distribution of CCK-8-, NPY-, Leu-ENK-, and Ghrelin- in the digestive tract of Prochilodus lineatus (Valenciennes, 1836) Barrios, Carlos Eduardo Santinón, Juan José Domitrovic, Hugo Alberto Sánchez, Sebastián Henández, David Roque Detritivorous fish Sábalo Endocrine cells Immunohistochemistry Neuropeptide This study describes the histological characteristics and distribution of gastrointestinal tract endocrine cells (ECs) of Prochilodus lineatus (detritivorous fish) using immunohistochemical procedures. The digestive tract of P. lineatus was divided into seven portions: stomach (cardial and pyloric), pyloric caeca, and intestine (anterior, glandular, middle and posterior). A pool of specific antisera against cholecystokinin (CCK-8), -neuropeptide Y (NPY), -ghrelin (Ghre) and -leu-enkephalin (Leu-ENK) to identify ECs were used. According to the morphological characteristics of ECs, two different types were identified and classified as open or closed-type. The number of ECs varied throughout the gastrointestinal tract, though a high abundance was found in the anterior intestine and pyloric caeca. A large number of ECs immunoreactive to CCK-8 and NPY were recorded in the anterior, glandular and middle intestine. ECs immunopositive to Leu-ENK were distributed in the stomach and pyloric caeca. For Ghre, immunopositive ECs were restricted to the glandular intestine. The results of the present study indicate that P. lineatus presents an ECs distribution pattern with species-specific particularities. However, CCK showed a distribution similar to that of omnivores, which is possibly related to local signaling functions in order to achieve the correct digestion of the various organisms found in the detritus. 2024-02-27T10:54:46Z 2024-02-27T10:54:46Z 2020-12 Artículo Barrios, Carlos Eduardo, et al., 2020. Localization and distribution of CCK-8-, NPY-, Leu-ENK-, and Ghrelin- in the digestive tract of Prochilodus lineatus (Valenciennes, 1836). Anais da Academia Brasileira de Ciências. Río de Janeiro: Academia Brasileira de Ciencias, vol. 92, no. 2, p. 1-12. E-ISSN 1678-2690. 0001-3765 http://repositorio.unne.edu.ar/handle/123456789/53054 eng https://www.scielo.br/j/aabc/a/5W59vTKpwvvyjgXwCGyfYjP/?format=pdf&lang=en openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 1-12 application/pdf Academia Brasileira de Ciencias Anais da Academia Brasileira de Ciências, vol. 92, no. 2, p. 1-12.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Detritivorous fish
Sábalo
Endocrine cells
Immunohistochemistry
Neuropeptide
spellingShingle Detritivorous fish
Sábalo
Endocrine cells
Immunohistochemistry
Neuropeptide
Barrios, Carlos Eduardo
Santinón, Juan José
Domitrovic, Hugo Alberto
Sánchez, Sebastián
Henández, David Roque
Localization and distribution of CCK-8-, NPY-, Leu-ENK-, and Ghrelin- in the digestive tract of Prochilodus lineatus (Valenciennes, 1836)
topic_facet Detritivorous fish
Sábalo
Endocrine cells
Immunohistochemistry
Neuropeptide
description This study describes the histological characteristics and distribution of gastrointestinal tract endocrine cells (ECs) of Prochilodus lineatus (detritivorous fish) using immunohistochemical procedures. The digestive tract of P. lineatus was divided into seven portions: stomach (cardial and pyloric), pyloric caeca, and intestine (anterior, glandular, middle and posterior). A pool of specific antisera against cholecystokinin (CCK-8), -neuropeptide Y (NPY), -ghrelin (Ghre) and -leu-enkephalin (Leu-ENK) to identify ECs were used. According to the morphological characteristics of ECs, two different types were identified and classified as open or closed-type. The number of ECs varied throughout the gastrointestinal tract, though a high abundance was found in the anterior intestine and pyloric caeca. A large number of ECs immunoreactive to CCK-8 and NPY were recorded in the anterior, glandular and middle intestine. ECs immunopositive to Leu-ENK were distributed in the stomach and pyloric caeca. For Ghre, immunopositive ECs were restricted to the glandular intestine. The results of the present study indicate that P. lineatus presents an ECs distribution pattern with species-specific particularities. However, CCK showed a distribution similar to that of omnivores, which is possibly related to local signaling functions in order to achieve the correct digestion of the various organisms found in the detritus.
format Artículo
author Barrios, Carlos Eduardo
Santinón, Juan José
Domitrovic, Hugo Alberto
Sánchez, Sebastián
Henández, David Roque
author_facet Barrios, Carlos Eduardo
Santinón, Juan José
Domitrovic, Hugo Alberto
Sánchez, Sebastián
Henández, David Roque
author_sort Barrios, Carlos Eduardo
title Localization and distribution of CCK-8-, NPY-, Leu-ENK-, and Ghrelin- in the digestive tract of Prochilodus lineatus (Valenciennes, 1836)
title_short Localization and distribution of CCK-8-, NPY-, Leu-ENK-, and Ghrelin- in the digestive tract of Prochilodus lineatus (Valenciennes, 1836)
title_full Localization and distribution of CCK-8-, NPY-, Leu-ENK-, and Ghrelin- in the digestive tract of Prochilodus lineatus (Valenciennes, 1836)
title_fullStr Localization and distribution of CCK-8-, NPY-, Leu-ENK-, and Ghrelin- in the digestive tract of Prochilodus lineatus (Valenciennes, 1836)
title_full_unstemmed Localization and distribution of CCK-8-, NPY-, Leu-ENK-, and Ghrelin- in the digestive tract of Prochilodus lineatus (Valenciennes, 1836)
title_sort localization and distribution of cck-8-, npy-, leu-enk-, and ghrelin- in the digestive tract of prochilodus lineatus (valenciennes, 1836)
publisher Academia Brasileira de Ciencias
publishDate 2024
url http://repositorio.unne.edu.ar/handle/123456789/53054
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