In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species

The strict nutritional requirements of Malassezia species make it difficult to test the antifungal susceptibility. Treatments of the chronic and recurrent infections associ- ated with Malassezia spp. are usually ineffective. The objective of this study was to obtain in vitro susceptibility pr...

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Autores principales: Carrillo Muñoz, Alfonso Javier, Rojas, Florencia Dinorah, Tur Tur, Cristina, Sosa, María de los Ángeles, Ortiz Diez, Gustavo, Martin Espada, Carmen, Payá, María Jesús, Giusiano, Gustavo Emilio
Formato: Artículo
Lenguaje:Inglés
Publicado: Wiley 2023
Materias:
Malassezia
Susceptibility
Topical antifungals
Systemic antifungals
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/52710
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-52710
record_format dspace
spelling I48-R184-123456789-527102023-11-17T14:22:33Z In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species Carrillo Muñoz, Alfonso Javier Rojas, Florencia Dinorah Tur Tur, Cristina Sosa, María de los Ángeles Ortiz Diez, Gustavo Martin Espada, Carmen Payá, María Jesús Giusiano, Gustavo Emilio Malassezia Susceptibility Topical antifungals Systemic antifungals The strict nutritional requirements of Malassezia species make it difficult to test the antifungal susceptibility. Treatments of the chronic and recurrent infections associ- ated with Malassezia spp. are usually ineffective. The objective of this study was to obtain in vitro susceptibility profile of 76 clinical isolates of Malassezia species against 16 antifungal drugs used for topical or systemic treatment. Isolates were identified by restriction fragment length polymorphism. Minimal inhibitory concentrations (MIC) were obtained by a modified microdilution method based on the Clinical Labo- ratory Standards Institute reference document M27-A3. The modifications allowed a good growth of all tested species. High in vitro antifungal activity of most tested drugs was observed, especially triazole derivatives, except for fluconazole which pre- sented the highest MICs and widest range of concentrations. Ketoconazole and itrac- onazole demonstrated a great activity. Higher MICs values were obtained with Malassezia furfur indicating a low susceptibility to most of the antifungal agents tested. Malassezia sympodialis and Malassezia pachydermatis were found to be more- susceptible species than M. furfur, Malassezia globosa, Malassezia slooffiae and Malas- sezia restricta. Topical substances were also active but provide higher MICs than the compounds for systemic use. The differences observed in the antifungals activity and interspecies variability demonstrated the importance to studying the susceptibility profile of each species to obtain reliable information for defining an effective treat- ment regimen. 2023-10-09T12:24:48Z 2023-10-09T12:24:48Z 2013 Artículo Carrillo Muñoz, Alfonso Javier, et al., 2013. In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species. Mycoses. Hoboken: Wiley, vol. 56, no. 1, p. 571-575. ISSN 0933-7407. 0933-7407 http://repositorio.unne.edu.ar/handle/123456789/52710 eng openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 571-575 application/pdf Wiley Mycoses, 2013, vol. 56, no. 1, p. 571-575.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Malassezia
Susceptibility
Topical antifungals
Systemic antifungals
spellingShingle Malassezia
Susceptibility
Topical antifungals
Systemic antifungals
Carrillo Muñoz, Alfonso Javier
Rojas, Florencia Dinorah
Tur Tur, Cristina
Sosa, María de los Ángeles
Ortiz Diez, Gustavo
Martin Espada, Carmen
Payá, María Jesús
Giusiano, Gustavo Emilio
In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species
topic_facet Malassezia
Susceptibility
Topical antifungals
Systemic antifungals
description The strict nutritional requirements of Malassezia species make it difficult to test the antifungal susceptibility. Treatments of the chronic and recurrent infections associ- ated with Malassezia spp. are usually ineffective. The objective of this study was to obtain in vitro susceptibility profile of 76 clinical isolates of Malassezia species against 16 antifungal drugs used for topical or systemic treatment. Isolates were identified by restriction fragment length polymorphism. Minimal inhibitory concentrations (MIC) were obtained by a modified microdilution method based on the Clinical Labo- ratory Standards Institute reference document M27-A3. The modifications allowed a good growth of all tested species. High in vitro antifungal activity of most tested drugs was observed, especially triazole derivatives, except for fluconazole which pre- sented the highest MICs and widest range of concentrations. Ketoconazole and itrac- onazole demonstrated a great activity. Higher MICs values were obtained with Malassezia furfur indicating a low susceptibility to most of the antifungal agents tested. Malassezia sympodialis and Malassezia pachydermatis were found to be more- susceptible species than M. furfur, Malassezia globosa, Malassezia slooffiae and Malas- sezia restricta. Topical substances were also active but provide higher MICs than the compounds for systemic use. The differences observed in the antifungals activity and interspecies variability demonstrated the importance to studying the susceptibility profile of each species to obtain reliable information for defining an effective treat- ment regimen.
format Artículo
author Carrillo Muñoz, Alfonso Javier
Rojas, Florencia Dinorah
Tur Tur, Cristina
Sosa, María de los Ángeles
Ortiz Diez, Gustavo
Martin Espada, Carmen
Payá, María Jesús
Giusiano, Gustavo Emilio
author_facet Carrillo Muñoz, Alfonso Javier
Rojas, Florencia Dinorah
Tur Tur, Cristina
Sosa, María de los Ángeles
Ortiz Diez, Gustavo
Martin Espada, Carmen
Payá, María Jesús
Giusiano, Gustavo Emilio
author_sort Carrillo Muñoz, Alfonso Javier
title In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species
title_short In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species
title_full In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species
title_fullStr In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species
title_full_unstemmed In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species
title_sort in vitro antifungal activity of topical and systemic antifungal drugs against malassezia species
publisher Wiley
publishDate 2023
url http://repositorio.unne.edu.ar/handle/123456789/52710
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