Antifungal susceptibility of Malassezia furfur, Malassezia sympodialis, and Malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method

We studied the in vitro activity of fluconazole (FCZ), ketoconazole (KTZ), miconazole (MCZ), voriconazole (VCZ), itraconazole (ITZ) and amphotericin B (AMB) against the three major pathogenic Malassezia species, M. globosa, M. sympodialis, and M. furfur. Antifungal susceptibilities were determined u...

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Autores principales: Rojas, Florencia Dinorah, Sosa, María de los Ángeles, Fernández, Mariana Soledad, Cattana, María Emilia, Córdoba, Susana Beatriz, Giusiano, Gustavo Emilio
Formato: Artículo
Lenguaje:Inglés
Publicado: International Society for Human and Animal Mycology 2023
Materias:
Malassezia
Susceptibility
Broth microdilution
Azole drug
Amphotericin B.
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/52318
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-52318
record_format dspace
spelling I48-R184-123456789-523182023-10-09T12:11:06Z Antifungal susceptibility of Malassezia furfur, Malassezia sympodialis, and Malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method Rojas, Florencia Dinorah Sosa, María de los Ángeles Fernández, Mariana Soledad Cattana, María Emilia Córdoba, Susana Beatriz Giusiano, Gustavo Emilio Malassezia Susceptibility Broth microdilution Azole drug Amphotericin B. We studied the in vitro activity of fluconazole (FCZ), ketoconazole (KTZ), miconazole (MCZ), voriconazole (VCZ), itraconazole (ITZ) and amphotericin B (AMB) against the three major pathogenic Malassezia species, M. globosa, M. sympodialis, and M. furfur. Antifungal susceptibilities were determined using the broth microdilution method in accordance with Clinical and Laboratory Standards Institute reference document M27-A3. To support lipid-dependent yeast development, glucose, peptone, ox bile, malt extract, glycerol, and Tween supplements were added to Roswell Park Memorial Institute RPMI 1640 medium. The supplemented medium allowed good growth of all three species studied. The minimal inhibitory concentrations (MICs) were recorded after 72 h of incubation at 32oC. The three species showed different susceptibility profiles for the drugs tested. Malassezia sympodialis was the most susceptible and M. furfur the least susceptible species. KTZ, ITZ, and VCZ were the most active drugs, showing low variability among isolates of the same species. FCZ, MCZ, and AMB showed high MICs and wide MIC ranges. Differences observed emphasize the need to accurately identify and evaluate antifungal susceptibility of Malassezia species. Further investigations and collaborative studies are essential for correlating in vitro results with clinical outcomes since the existing limited data do not allow definitive conclusions. 2023-09-01T12:46:58Z 2023-09-01T12:46:58Z 2014 Artículo Rojas, Florencia Dinorah, et al., 2014. Antifungal susceptibility of Malassezia furfur, Malassezia sympodialis and Malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method. Medical Mycology. Oxford: International Society for Human and Animal Mycology, vol. 52, no. 6, p. 641-646. E-ISSN 1460-2709. http://repositorio.unne.edu.ar/handle/123456789/52318 eng openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 641-646 application/pdf International Society for Human and Animal Mycology Medical Mycology, 2014, vol. 52, no. 6, p. 641-646.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Malassezia
Susceptibility
Broth microdilution
Azole drug
Amphotericin B.
spellingShingle Malassezia
Susceptibility
Broth microdilution
Azole drug
Amphotericin B.
Rojas, Florencia Dinorah
Sosa, María de los Ángeles
Fernández, Mariana Soledad
Cattana, María Emilia
Córdoba, Susana Beatriz
Giusiano, Gustavo Emilio
Antifungal susceptibility of Malassezia furfur, Malassezia sympodialis, and Malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method
topic_facet Malassezia
Susceptibility
Broth microdilution
Azole drug
Amphotericin B.
description We studied the in vitro activity of fluconazole (FCZ), ketoconazole (KTZ), miconazole (MCZ), voriconazole (VCZ), itraconazole (ITZ) and amphotericin B (AMB) against the three major pathogenic Malassezia species, M. globosa, M. sympodialis, and M. furfur. Antifungal susceptibilities were determined using the broth microdilution method in accordance with Clinical and Laboratory Standards Institute reference document M27-A3. To support lipid-dependent yeast development, glucose, peptone, ox bile, malt extract, glycerol, and Tween supplements were added to Roswell Park Memorial Institute RPMI 1640 medium. The supplemented medium allowed good growth of all three species studied. The minimal inhibitory concentrations (MICs) were recorded after 72 h of incubation at 32oC. The three species showed different susceptibility profiles for the drugs tested. Malassezia sympodialis was the most susceptible and M. furfur the least susceptible species. KTZ, ITZ, and VCZ were the most active drugs, showing low variability among isolates of the same species. FCZ, MCZ, and AMB showed high MICs and wide MIC ranges. Differences observed emphasize the need to accurately identify and evaluate antifungal susceptibility of Malassezia species. Further investigations and collaborative studies are essential for correlating in vitro results with clinical outcomes since the existing limited data do not allow definitive conclusions.
format Artículo
author Rojas, Florencia Dinorah
Sosa, María de los Ángeles
Fernández, Mariana Soledad
Cattana, María Emilia
Córdoba, Susana Beatriz
Giusiano, Gustavo Emilio
author_facet Rojas, Florencia Dinorah
Sosa, María de los Ángeles
Fernández, Mariana Soledad
Cattana, María Emilia
Córdoba, Susana Beatriz
Giusiano, Gustavo Emilio
author_sort Rojas, Florencia Dinorah
title Antifungal susceptibility of Malassezia furfur, Malassezia sympodialis, and Malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method
title_short Antifungal susceptibility of Malassezia furfur, Malassezia sympodialis, and Malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method
title_full Antifungal susceptibility of Malassezia furfur, Malassezia sympodialis, and Malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method
title_fullStr Antifungal susceptibility of Malassezia furfur, Malassezia sympodialis, and Malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method
title_full_unstemmed Antifungal susceptibility of Malassezia furfur, Malassezia sympodialis, and Malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method
title_sort antifungal susceptibility of malassezia furfur, malassezia sympodialis, and malassezia globosa to azole drugs and amphotericin b evaluated using a broth microdilution method
publisher International Society for Human and Animal Mycology
publishDate 2023
url http://repositorio.unne.edu.ar/handle/123456789/52318
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