Effects of filgrastim on granulopoietic cells of mice pretreated with methotrexate

We have evaluated the effect of filgrastim on proliferation and differentiation activity of granulopoietic cells in mice pretreated with methotrexate. Filgrastim was injected daily, from day 8 to 28 after cytotoxic agent administration. The granulopoiesis changes were measured by assessment of GM-CF...

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Detalles Bibliográficos
Autores principales: Barrios, Lilián, Poletti, Oscar Héctor
Formato: Artículo
Lenguaje:Inglés
Publicado: Sociedad Latinoamericana de Microscopia Electrónica 2023
Materias:
Methotrexate
Filgrastim
Granulopoiesis
Bone marrow
Spleen
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/51386
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-51386
record_format dspace
spelling I48-R184-123456789-513862024-12-16T11:34:38Z Effects of filgrastim on granulopoietic cells of mice pretreated with methotrexate Barrios, Lilián Poletti, Oscar Héctor Methotrexate Filgrastim Granulopoiesis Bone marrow Spleen We have evaluated the effect of filgrastim on proliferation and differentiation activity of granulopoietic cells in mice pretreated with methotrexate. Filgrastim was injected daily, from day 8 to 28 after cytotoxic agent administration. The granulopoiesis changes were measured by assessment of GM-CFU cells content, marrow and spleen granuloid cells pool as well as circulating neutrophils. In MTX pretreated mice, bone marrow GM-CFU oscillating values were higher than normal values, but these changes were not followed by high proliferative activity in granuloid precursor cell compartment. After MTX treatment, filgrastim administration was unable to stimulate marrow granulopoiesis as observed in normal mice. In the spleen, MTX led to dramatic changes in the proliferative activity of GM-CFU cells, but did not result in spleen granuloid cell changes. However, filgrastim treatment induced a spleen granuloid amplification, similar to the changes observed in circulating neutrophils values. We suggest that these findings can be explained by inhibition of differentiation of marrow GM-CFU cells into the more mature granulopoietic cells and/or by an inhibited proliferative activity of marrow granuloid cells. They can be also explained in terms of an unfavorable marrow microenvironment for granulopoiesis, contrary to a supportive spleen microenvironment. 2023-05-03T11:08:14Z 2023-05-03T11:08:14Z 2005 Artículo Barrios, Lilián y Poletti, Oscar Héctor, 2010. Effects of filgrastim on granulopoietic cells of mice pretreated with methotrexate. Biocell. Mendoza: Sociedad Latinoamericana de Microscopia Electrónica, vol. 29, no. 1, p. 7-14. E-ISSN 1667-5746. 0327-9545 http://repositorio.unne.edu.ar/handle/123456789/51386 eng openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 7-14 application/pdf Sociedad Latinoamericana de Microscopia Electrónica Biocell, 2005, vol. 29, no. 1, p. 7-14.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Methotrexate
Filgrastim
Granulopoiesis
Bone marrow
Spleen
spellingShingle Methotrexate
Filgrastim
Granulopoiesis
Bone marrow
Spleen
Barrios, Lilián
Poletti, Oscar Héctor
Effects of filgrastim on granulopoietic cells of mice pretreated with methotrexate
topic_facet Methotrexate
Filgrastim
Granulopoiesis
Bone marrow
Spleen
description We have evaluated the effect of filgrastim on proliferation and differentiation activity of granulopoietic cells in mice pretreated with methotrexate. Filgrastim was injected daily, from day 8 to 28 after cytotoxic agent administration. The granulopoiesis changes were measured by assessment of GM-CFU cells content, marrow and spleen granuloid cells pool as well as circulating neutrophils. In MTX pretreated mice, bone marrow GM-CFU oscillating values were higher than normal values, but these changes were not followed by high proliferative activity in granuloid precursor cell compartment. After MTX treatment, filgrastim administration was unable to stimulate marrow granulopoiesis as observed in normal mice. In the spleen, MTX led to dramatic changes in the proliferative activity of GM-CFU cells, but did not result in spleen granuloid cell changes. However, filgrastim treatment induced a spleen granuloid amplification, similar to the changes observed in circulating neutrophils values. We suggest that these findings can be explained by inhibition of differentiation of marrow GM-CFU cells into the more mature granulopoietic cells and/or by an inhibited proliferative activity of marrow granuloid cells. They can be also explained in terms of an unfavorable marrow microenvironment for granulopoiesis, contrary to a supportive spleen microenvironment.
format Artículo
author Barrios, Lilián
Poletti, Oscar Héctor
author_facet Barrios, Lilián
Poletti, Oscar Héctor
author_sort Barrios, Lilián
title Effects of filgrastim on granulopoietic cells of mice pretreated with methotrexate
title_short Effects of filgrastim on granulopoietic cells of mice pretreated with methotrexate
title_full Effects of filgrastim on granulopoietic cells of mice pretreated with methotrexate
title_fullStr Effects of filgrastim on granulopoietic cells of mice pretreated with methotrexate
title_full_unstemmed Effects of filgrastim on granulopoietic cells of mice pretreated with methotrexate
title_sort effects of filgrastim on granulopoietic cells of mice pretreated with methotrexate
publisher Sociedad Latinoamericana de Microscopia Electrónica
publishDate 2023
url http://repositorio.unne.edu.ar/handle/123456789/51386
work_keys_str_mv AT barrioslilian effectsoffilgrastimongranulopoieticcellsofmicepretreatedwithmethotrexate
AT polettioscarhector effectsoffilgrastimongranulopoieticcellsofmicepretreatedwithmethotrexate
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