Plant regeneration of ilex paraguariensis (aquifoliaceaé) by in vitro culture of nodal segments

In vitro regeneration of complete plants ffom nodal single bud segments of “yerba mate” {Ilex paraguariensis St. Hil.) was studied under defined nutritional and environmental conditions. Nodal segments harvested ffom actively growing shoots o f conventionally raised plants were cultured on nutrient...

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Autores principales: Sansberro, Pedro Alfonso, Rey, Hebe Yolanda, Bernardis, Aldo Ceferino, Luna, Claudia Verónica, Collavino, Mónica Mariana, Mroginski, Luis Amado
Formato: Artículo
Lenguaje:Inglés
Publicado: Sociedades Latinoamericanas de Microscopia Electrónica. Centro Regional de Investigaciones Científicas y Tecnológicas 2022
Materias:
Regeneration
Ilex paraguariensis
Micropropagation
Nodal segment
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/48875
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-48875
record_format dspace
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Regeneration
Ilex paraguariensis
Micropropagation
Nodal segment
spellingShingle Regeneration
Ilex paraguariensis
Micropropagation
Nodal segment
Sansberro, Pedro Alfonso
Rey, Hebe Yolanda
Bernardis, Aldo Ceferino
Luna, Claudia Verónica
Collavino, Mónica Mariana
Mroginski, Luis Amado
Plant regeneration of ilex paraguariensis (aquifoliaceaé) by in vitro culture of nodal segments
topic_facet Regeneration
Ilex paraguariensis
Micropropagation
Nodal segment
description In vitro regeneration of complete plants ffom nodal single bud segments of “yerba mate” {Ilex paraguariensis St. Hil.) was studied under defined nutritional and environmental conditions. Nodal segments harvested ffom actively growing shoots o f conventionally raised plants were cultured on nutrient médium with the mineral salts and vitamins o f Murashige and Skoog médium at 1/4 strength, supplemented with various concentrations o f sucrose and 6-benzyladenine (BAP). Shoot regeneration ffom explants o f both young (2 years oíd) and adult (20 years oíd) mother plants were readily achieved in the médium supplemented with 0.04 - 0.09 M sucrose with or without BAP. As many as 60 - 65% o f the nodal segments cultured formed shoots. Rooting o f regenerated shoots was observed in 50% o f the explants harvested ffom young plants, whereas 25% o f the explants rooted when the nodal explants were harvested ffom adult plants. The best rooting induction was achieved on 1/4 strength MS médium with vermiculite as the substrate and supplemented with 1 - 1.5% IBA (indolebutyric acid) and 1 - 2% PPZ (3- methyl - 1 - phenyl - 2 pyrazolin - 5 - one). Plantlets were successfully transferred to soil.
format Artículo
author Sansberro, Pedro Alfonso
Rey, Hebe Yolanda
Bernardis, Aldo Ceferino
Luna, Claudia Verónica
Collavino, Mónica Mariana
Mroginski, Luis Amado
author_facet Sansberro, Pedro Alfonso
Rey, Hebe Yolanda
Bernardis, Aldo Ceferino
Luna, Claudia Verónica
Collavino, Mónica Mariana
Mroginski, Luis Amado
author_sort Sansberro, Pedro Alfonso
title Plant regeneration of ilex paraguariensis (aquifoliaceaé) by in vitro culture of nodal segments
title_short Plant regeneration of ilex paraguariensis (aquifoliaceaé) by in vitro culture of nodal segments
title_full Plant regeneration of ilex paraguariensis (aquifoliaceaé) by in vitro culture of nodal segments
title_fullStr Plant regeneration of ilex paraguariensis (aquifoliaceaé) by in vitro culture of nodal segments
title_full_unstemmed Plant regeneration of ilex paraguariensis (aquifoliaceaé) by in vitro culture of nodal segments
title_sort plant regeneration of ilex paraguariensis (aquifoliaceaé) by in vitro culture of nodal segments
publisher Sociedades Latinoamericanas de Microscopia Electrónica. Centro Regional de Investigaciones Científicas y Tecnológicas
publishDate 2022
url http://repositorio.unne.edu.ar/handle/123456789/48875
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AT bernardisaldoceferino plantregenerationofilexparaguariensisaquifoliaceaebyinvitrocultureofnodalsegments
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