Analysis of the clonal relationship among clinical isolates of salmonella enterica serovar infantis by different typing methods

Salmonella Infantis has been the second most common serovar in Argentina in the last two years, being isolated mostly from paediatric hospitalised patients. In order to determine the clonal relationship among Salmonella Infantis strains, we examined 15 isolates from paediatric patient faeces in Arge...

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Autores principales: Merino, Luis Antonio, Ronconi, María Cristina, Navia, Margarita M., Ruiz, Joaquim, Sierra, Josep M., Cech, Norma B., Lodeiro, Norma S., Vila, Jordi
Formato: Artículo
Lenguaje:Inglés
Publicado: Instituto de Medicina Tropical de São Paulo 2022
Materias:
Salmonella Infantis
Typing
Pulse Field Gel Electrophoresis
Polymerase Chain Reaction
Argentina
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/48858
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-48858
record_format dspace
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Salmonella Infantis
Typing
Pulse Field Gel Electrophoresis
Polymerase Chain Reaction
Argentina
spellingShingle Salmonella Infantis
Typing
Pulse Field Gel Electrophoresis
Polymerase Chain Reaction
Argentina
Merino, Luis Antonio
Ronconi, María Cristina
Navia, Margarita M.
Ruiz, Joaquim
Sierra, Josep M.
Cech, Norma B.
Lodeiro, Norma S.
Vila, Jordi
Analysis of the clonal relationship among clinical isolates of salmonella enterica serovar infantis by different typing methods
topic_facet Salmonella Infantis
Typing
Pulse Field Gel Electrophoresis
Polymerase Chain Reaction
Argentina
description Salmonella Infantis has been the second most common serovar in Argentina in the last two years, being isolated mostly from paediatric hospitalised patients. In order to determine the clonal relationship among Salmonella Infantis strains, we examined 15 isolates from paediatric patient faeces in Argentina (12 geographically related and 3 geographically non-related) by using antimicrobial susceptibility, plasmid profiling, repetitive extragenic palindromic (REP) PCR, enterobacterial repetitive intergenic consensus (ERIC) PCR, and low-frequency restriction analysis of chromosomal DNA by pulsed field gel electrophoresis (PFGE). Four Spanish strains were included as controls of clonal diversity in molecular techniques. Antibiotype and plasmid profile was not useful as epidemiological tools. PFGE and REP-PCR were able to discriminate between Argentinean and Spanish isolates of Salmonella Infantis allowing to detect genetically related strains in three different cities. This finding indicates that a possible spread of a clone of this serovar in the North-eastern Region of Argentina has taken place in 1998.
format Artículo
author Merino, Luis Antonio
Ronconi, María Cristina
Navia, Margarita M.
Ruiz, Joaquim
Sierra, Josep M.
Cech, Norma B.
Lodeiro, Norma S.
Vila, Jordi
author_facet Merino, Luis Antonio
Ronconi, María Cristina
Navia, Margarita M.
Ruiz, Joaquim
Sierra, Josep M.
Cech, Norma B.
Lodeiro, Norma S.
Vila, Jordi
author_sort Merino, Luis Antonio
title Analysis of the clonal relationship among clinical isolates of salmonella enterica serovar infantis by different typing methods
title_short Analysis of the clonal relationship among clinical isolates of salmonella enterica serovar infantis by different typing methods
title_full Analysis of the clonal relationship among clinical isolates of salmonella enterica serovar infantis by different typing methods
title_fullStr Analysis of the clonal relationship among clinical isolates of salmonella enterica serovar infantis by different typing methods
title_full_unstemmed Analysis of the clonal relationship among clinical isolates of salmonella enterica serovar infantis by different typing methods
title_sort analysis of the clonal relationship among clinical isolates of salmonella enterica serovar infantis by different typing methods
publisher Instituto de Medicina Tropical de São Paulo
publishDate 2022
url http://repositorio.unne.edu.ar/handle/123456789/48858
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