Plant regeneration from ilex spp. (aquifoliaceae) in vitro

In vitro plant regeneration from nodal segments (containing one axillary bud) of seven species of the genus Ilex (l argentina, I. brevicuspis, I. dumosa, I. microdonta, I. pseudoboxus, I. taubertianb and I theezans ) were readily achieved through three steps: 1) shoot regeneration by in vitro cultur...

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Autores principales: Sansberro, Pedro Alfonso, Rey, Hebe Yolanda, Mroginski, Luis Amado, Krivenki, Mario Angel
Formato: Artículo
Lenguaje:Español
Publicado: Sociedad Latinoamericana de Microscopía Electrónica. Centro Regional de Investigaciones Científicas y Tecnológicas Mendoza 2022
Materias:
Plant regeneration
Germination
Embryo culture
Holly
Propagation
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/48649
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-48649
record_format dspace
spelling I48-R184-123456789-486492025-10-15T11:55:31Z Plant regeneration from ilex spp. (aquifoliaceae) in vitro Sansberro, Pedro Alfonso Rey, Hebe Yolanda Mroginski, Luis Amado Krivenki, Mario Angel Plant regeneration Germination Embryo culture Holly Propagation In vitro plant regeneration from nodal segments (containing one axillary bud) of seven species of the genus Ilex (l argentina, I. brevicuspis, I. dumosa, I. microdonta, I. pseudoboxus, I. taubertianb and I theezans ) were readily achieved through three steps: 1) shoot regeneration by in vitro culture of nodal segments in MS médium at 1/4 strength, plus 3% sucrose and 0.65% agar (1/4MS) and 0.5 |jM BA (45 days of culture); 2) Induction of rooting from regenerated shoots with 1/4MS (solidified with 2.5 g.L'1 “Phytagel”) with 7.3 pM IBA (7 days) and, 3) subculture of shoot on a fresh médium (1/4MS lacking plant growth regulators) during 21 days. Shoot regeneration of other three species ( I aquifolium, /. bmsiliensis and /. inlegerrima) were also obtained by in vitro culture of nodal segments. Shoot regeneration of/. aquifolium, I. brasiliensis, I. integerrima, I. microdonta, I. pseudoboxus, and I. taubertiana were also obtained by culture shoot tips on 1/4MS and 0.5 ]iM BA. Shoot regeneration from meristems o f l argentina, I. brevicuspis, I. dumosa, and I. theezans were readily achieved by in vitro culture on the same médium. 2022-07-04T14:21:50Z 2022-07-04T14:21:50Z 2001 Artículo Sansberro, Pedro Alfonso, et al., 2001. Plant Regeneration from ilex spp. (aquifoliaceae) in vitro. Biocell. Mendoza: Sociedad Latinoamericana de Microscopía Electrónica. Centro Regional de Investigaciones Científicas y Tecnológicas Mendoza, vol. 25, no. 2, p. 139-146. ISSN-e 1667-5746. 0327-9545 http://repositorio.unne.edu.ar/handle/123456789/48649 spa openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf application/pdf Sociedad Latinoamericana de Microscopía Electrónica. Centro Regional de Investigaciones Científicas y Tecnológicas Mendoza Biocell, 2001, vol. 25 no. 2, p. 139-146.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Español
topic Plant regeneration
Germination
Embryo culture
Holly
Propagation
spellingShingle Plant regeneration
Germination
Embryo culture
Holly
Propagation
Sansberro, Pedro Alfonso
Rey, Hebe Yolanda
Mroginski, Luis Amado
Krivenki, Mario Angel
Plant regeneration from ilex spp. (aquifoliaceae) in vitro
topic_facet Plant regeneration
Germination
Embryo culture
Holly
Propagation
description In vitro plant regeneration from nodal segments (containing one axillary bud) of seven species of the genus Ilex (l argentina, I. brevicuspis, I. dumosa, I. microdonta, I. pseudoboxus, I. taubertianb and I theezans ) were readily achieved through three steps: 1) shoot regeneration by in vitro culture of nodal segments in MS médium at 1/4 strength, plus 3% sucrose and 0.65% agar (1/4MS) and 0.5 |jM BA (45 days of culture); 2) Induction of rooting from regenerated shoots with 1/4MS (solidified with 2.5 g.L'1 “Phytagel”) with 7.3 pM IBA (7 days) and, 3) subculture of shoot on a fresh médium (1/4MS lacking plant growth regulators) during 21 days. Shoot regeneration of other three species ( I aquifolium, /. bmsiliensis and /. inlegerrima) were also obtained by in vitro culture of nodal segments. Shoot regeneration of/. aquifolium, I. brasiliensis, I. integerrima, I. microdonta, I. pseudoboxus, and I. taubertiana were also obtained by culture shoot tips on 1/4MS and 0.5 ]iM BA. Shoot regeneration from meristems o f l argentina, I. brevicuspis, I. dumosa, and I. theezans were readily achieved by in vitro culture on the same médium.
format Artículo
author Sansberro, Pedro Alfonso
Rey, Hebe Yolanda
Mroginski, Luis Amado
Krivenki, Mario Angel
author_facet Sansberro, Pedro Alfonso
Rey, Hebe Yolanda
Mroginski, Luis Amado
Krivenki, Mario Angel
author_sort Sansberro, Pedro Alfonso
title Plant regeneration from ilex spp. (aquifoliaceae) in vitro
title_short Plant regeneration from ilex spp. (aquifoliaceae) in vitro
title_full Plant regeneration from ilex spp. (aquifoliaceae) in vitro
title_fullStr Plant regeneration from ilex spp. (aquifoliaceae) in vitro
title_full_unstemmed Plant regeneration from ilex spp. (aquifoliaceae) in vitro
title_sort plant regeneration from ilex spp. (aquifoliaceae) in vitro
publisher Sociedad Latinoamericana de Microscopía Electrónica. Centro Regional de Investigaciones Científicas y Tecnológicas Mendoza
publishDate 2022
url http://repositorio.unne.edu.ar/handle/123456789/48649
work_keys_str_mv AT sansberropedroalfonso plantregenerationfromilexsppaquifoliaceaeinvitro
AT reyhebeyolanda plantregenerationfromilexsppaquifoliaceaeinvitro
AT mroginskiluisamado plantregenerationfromilexsppaquifoliaceaeinvitro
AT krivenkimarioangel plantregenerationfromilexsppaquifoliaceaeinvitro
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