Effect of morphological heterogeneity of somatic embryos of melia azedarach on conversion into plants

Embryogenic cultures were initiated from immature Melia azedarach (Meliaceae) zigotic embryos. Explants were induced on Murashige and Skoog (1962) medium with 4.54 μM thidiazuron or 0.45 μM dichlorophenoxyacetic acid. After 6 weeks of culture on induction medium, somatic embryos were categorized...

Descripción completa

Detalles Bibliográficos
Autores principales: Vila, Silvia, González, Ana María, Rey, Hebe Yolanda, Mroginski, Luis Amado
Formato: Artículo
Lenguaje:Español
Publicado: Sociedad Latinoamericana de Microscopia Electrónica 2022
Materias:
Somatic embryo morphology
Conversion
Histology
Scanning electron microscopy
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/48614
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-48614
record_format dspace
spelling I48-R184-123456789-486142024-09-30T13:47:09Z Effect of morphological heterogeneity of somatic embryos of melia azedarach on conversion into plants Vila, Silvia González, Ana María Rey, Hebe Yolanda Mroginski, Luis Amado Somatic embryo morphology Conversion Histology Scanning electron microscopy Embryogenic cultures were initiated from immature Melia azedarach (Meliaceae) zigotic embryos. Explants were induced on Murashige and Skoog (1962) medium with 4.54 μM thidiazuron or 0.45 μM dichlorophenoxyacetic acid. After 6 weeks of culture on induction medium, somatic embryos were categorized in four morphological classes based on the presence of single or fused embryos and if they remained united or not to the original explant; that were evaluated histologically. The somatic embryos of every category were transferred, in groups or individually, on a 1/4 MS medium. Bipolar embryos, the more typically normal ones, had well defined shoot and root apical meristems and produced single plants; subcultured individually their conversion was 28%, and subcultured in groups the conversion declined to 6.8%. Fused embryos subcultured in groups had only a 2.1% conversion and produced plants with fused stems. None conver- sion rate in the others classes was associated to poorly developed shoot and root meristematic areas or with their absence. The converted plants were acclimatized and transferred, in a mist, to soil, with an independent of the class 95% survival rate. 2022-06-30T20:07:44Z 2022-06-30T20:07:44Z 2010-01-16 Artículo Vila, Silvia, et al., 2010. Effect of morphological heterogeneity of somatic embryos of melia azedarach on conversion into plants. Biocell. Mendoza: Sociedad Latinoamericana de Microscopia Electrónica, vol. 34, no. 1, p. 7-13. e-ISSN 1667-5746. 0327-9545 http://repositorio.unne.edu.ar/handle/123456789/48614 spa https://www.scielo.org.ar/pdf/biocell/v34n1/v34n1a02.pdf openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf application/pdf Sociedad Latinoamericana de Microscopia Electrónica Biocell, 2010, vol. 34, no. 1, p. 7-13.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Español
topic Somatic embryo morphology
Conversion
Histology
Scanning electron microscopy
spellingShingle Somatic embryo morphology
Conversion
Histology
Scanning electron microscopy
Vila, Silvia
González, Ana María
Rey, Hebe Yolanda
Mroginski, Luis Amado
Effect of morphological heterogeneity of somatic embryos of melia azedarach on conversion into plants
topic_facet Somatic embryo morphology
Conversion
Histology
Scanning electron microscopy
description Embryogenic cultures were initiated from immature Melia azedarach (Meliaceae) zigotic embryos. Explants were induced on Murashige and Skoog (1962) medium with 4.54 μM thidiazuron or 0.45 μM dichlorophenoxyacetic acid. After 6 weeks of culture on induction medium, somatic embryos were categorized in four morphological classes based on the presence of single or fused embryos and if they remained united or not to the original explant; that were evaluated histologically. The somatic embryos of every category were transferred, in groups or individually, on a 1/4 MS medium. Bipolar embryos, the more typically normal ones, had well defined shoot and root apical meristems and produced single plants; subcultured individually their conversion was 28%, and subcultured in groups the conversion declined to 6.8%. Fused embryos subcultured in groups had only a 2.1% conversion and produced plants with fused stems. None conver- sion rate in the others classes was associated to poorly developed shoot and root meristematic areas or with their absence. The converted plants were acclimatized and transferred, in a mist, to soil, with an independent of the class 95% survival rate.
format Artículo
author Vila, Silvia
González, Ana María
Rey, Hebe Yolanda
Mroginski, Luis Amado
author_facet Vila, Silvia
González, Ana María
Rey, Hebe Yolanda
Mroginski, Luis Amado
author_sort Vila, Silvia
title Effect of morphological heterogeneity of somatic embryos of melia azedarach on conversion into plants
title_short Effect of morphological heterogeneity of somatic embryos of melia azedarach on conversion into plants
title_full Effect of morphological heterogeneity of somatic embryos of melia azedarach on conversion into plants
title_fullStr Effect of morphological heterogeneity of somatic embryos of melia azedarach on conversion into plants
title_full_unstemmed Effect of morphological heterogeneity of somatic embryos of melia azedarach on conversion into plants
title_sort effect of morphological heterogeneity of somatic embryos of melia azedarach on conversion into plants
publisher Sociedad Latinoamericana de Microscopia Electrónica
publishDate 2022
url http://repositorio.unne.edu.ar/handle/123456789/48614
work_keys_str_mv AT vilasilvia effectofmorphologicalheterogeneityofsomaticembryosofmeliaazedarachonconversionintoplants
AT gonzalezanamaria effectofmorphologicalheterogeneityofsomaticembryosofmeliaazedarachonconversionintoplants
AT reyhebeyolanda effectofmorphologicalheterogeneityofsomaticembryosofmeliaazedarachonconversionintoplants
AT mroginskiluisamado effectofmorphologicalheterogeneityofsomaticembryosofmeliaazedarachonconversionintoplants
_version_ 1832344138194878464

Ejemplares similares