Organogenesis and plant regeneration of arachis villosa benth. (leguminosae) through leaf culture
With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators: αaphthalenacetic acid,...
Guardado en:
| Autores principales: | , , |
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| Formato: | Artículo |
| Lenguaje: | Español |
| Publicado: |
Sociedad Latinoamericana de Microscopia Electrónica
2022
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| Materias: | |
| Acceso en línea: | http://repositorio.unne.edu.ar/handle/123456789/48613 |
| Aporte de: |
| Sumario: | With the aim of developing an efficient plant regeneration protocol, leaflet explants of three
accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog
medium supplemented with different combinations of plant growth regulators: αaphthalenacetic acid, in-
dole-3-butyric acid, 6-benzylaminopurine, kinetin and thidiazuron. The accession L97 was the only one able
to differentiate buds through indirect organogenesis. The most suitable combination for bud regeneration was
the basic medium added with 13.62 μM thidiazuron and 4.44 μM 6-benzylaminopurine. These results show
the important role of the genotype in morphogenetic responses and the organogenetic effect of thidiazuron in
Arachis villosa accession L97. A thidiazuron lacking media (only 0.54 μM α-naphthalenacetic acid, 13.95
μM kinetin and 13.32 μM 6-benzylaminopurine were added) promoted the elongation of the regenerated
buds. Adventitious rooting was achieved 90 days after the isolated shoots were transferred to a rooting medium containing 0.54 μM α-naphthalenacetic acid. |
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