Organogenesis and plant regeneration of arachis villosa benth. (leguminosae) through leaf culture
With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators: αaphthalenacetic acid,...
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| Formato: | Artículo |
| Lenguaje: | Español |
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Sociedad Latinoamericana de Microscopia Electrónica
2022
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| Acceso en línea: | http://repositorio.unne.edu.ar/handle/123456789/48613 |
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I48-R184-123456789-48613 |
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I48-R184-123456789-486132024-10-10T13:03:54Z Organogenesis and plant regeneration of arachis villosa benth. (leguminosae) through leaf culture Fontana, María Laura Mroginski, Luis Amado Rey, Hebe Yolanda In vitro cultivation Leaflet explants Micropropagation Thidiazuron Wild groundnut With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators: αaphthalenacetic acid, in- dole-3-butyric acid, 6-benzylaminopurine, kinetin and thidiazuron. The accession L97 was the only one able to differentiate buds through indirect organogenesis. The most suitable combination for bud regeneration was the basic medium added with 13.62 μM thidiazuron and 4.44 μM 6-benzylaminopurine. These results show the important role of the genotype in morphogenetic responses and the organogenetic effect of thidiazuron in Arachis villosa accession L97. A thidiazuron lacking media (only 0.54 μM α-naphthalenacetic acid, 13.95 μM kinetin and 13.32 μM 6-benzylaminopurine were added) promoted the elongation of the regenerated buds. Adventitious rooting was achieved 90 days after the isolated shoots were transferred to a rooting medium containing 0.54 μM α-naphthalenacetic acid. 2022-06-30T20:05:18Z 2022-06-30T20:05:18Z 2009-11-02 Artículo Fontana, María Laura, Mroginski Luis Amado y Rey, Hebe Yolanda, 2009. Organogenesis and plant regeneration of arachis villosa benth. (leguminosae) through leaf culture. Biocell. Mendoza: Sociedad Latinoamericana de Microscopia Electrónica, vol. 33, no. 3, p. 179-186. e-ISSN 1667-5746. 0327-9545 http://repositorio.unne.edu.ar/handle/123456789/48613 spa https://www.techscience.com/biocell/v33n3/37769/pdf openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf application/pdf Sociedad Latinoamericana de Microscopia Electrónica Biocell, 2009, vol. 33, no. 3, p. 179-186. |
| institution |
Universidad Nacional del Nordeste |
| institution_str |
I-48 |
| repository_str |
R-184 |
| collection |
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) |
| language |
Español |
| topic |
In vitro cultivation Leaflet explants Micropropagation Thidiazuron Wild groundnut |
| spellingShingle |
In vitro cultivation Leaflet explants Micropropagation Thidiazuron Wild groundnut Fontana, María Laura Mroginski, Luis Amado Rey, Hebe Yolanda Organogenesis and plant regeneration of arachis villosa benth. (leguminosae) through leaf culture |
| topic_facet |
In vitro cultivation Leaflet explants Micropropagation Thidiazuron Wild groundnut |
| description |
With the aim of developing an efficient plant regeneration protocol, leaflet explants of three
accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog
medium supplemented with different combinations of plant growth regulators: αaphthalenacetic acid, in-
dole-3-butyric acid, 6-benzylaminopurine, kinetin and thidiazuron. The accession L97 was the only one able
to differentiate buds through indirect organogenesis. The most suitable combination for bud regeneration was
the basic medium added with 13.62 μM thidiazuron and 4.44 μM 6-benzylaminopurine. These results show
the important role of the genotype in morphogenetic responses and the organogenetic effect of thidiazuron in
Arachis villosa accession L97. A thidiazuron lacking media (only 0.54 μM α-naphthalenacetic acid, 13.95
μM kinetin and 13.32 μM 6-benzylaminopurine were added) promoted the elongation of the regenerated
buds. Adventitious rooting was achieved 90 days after the isolated shoots were transferred to a rooting medium containing 0.54 μM α-naphthalenacetic acid. |
| format |
Artículo |
| author |
Fontana, María Laura Mroginski, Luis Amado Rey, Hebe Yolanda |
| author_facet |
Fontana, María Laura Mroginski, Luis Amado Rey, Hebe Yolanda |
| author_sort |
Fontana, María Laura |
| title |
Organogenesis and plant regeneration of arachis villosa benth. (leguminosae) through leaf culture |
| title_short |
Organogenesis and plant regeneration of arachis villosa benth. (leguminosae) through leaf culture |
| title_full |
Organogenesis and plant regeneration of arachis villosa benth. (leguminosae) through leaf culture |
| title_fullStr |
Organogenesis and plant regeneration of arachis villosa benth. (leguminosae) through leaf culture |
| title_full_unstemmed |
Organogenesis and plant regeneration of arachis villosa benth. (leguminosae) through leaf culture |
| title_sort |
organogenesis and plant regeneration of arachis villosa benth. (leguminosae) through leaf culture |
| publisher |
Sociedad Latinoamericana de Microscopia Electrónica |
| publishDate |
2022 |
| url |
http://repositorio.unne.edu.ar/handle/123456789/48613 |
| work_keys_str_mv |
AT fontanamarialaura organogenesisandplantregenerationofarachisvillosabenthleguminosaethroughleafculture AT mroginskiluisamado organogenesisandplantregenerationofarachisvillosabenthleguminosaethroughleafculture AT reyhebeyolanda organogenesisandplantregenerationofarachisvillosabenthleguminosaethroughleafculture |
| _version_ |
1832343688854896640 |