In vitro effect of chrysosporium indicum and chrysosporium keratinophylum on toxocara canis eggs
The degree of antagonism exercised by fungi on geohelminth development varies according to the morphological alterations caused by different fungal species. Saprophytic fungi may exert ovicidal or ovistatic effects. The aim of this study was to apply scanning electron microscopy (SEM) to observe...
Guardado en:
| Autores principales: | , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Asociación Argentina de Microbiología
2022
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| Materias: | |
| Acceso en línea: | http://repositorio.unne.edu.ar/handle/123456789/48511 |
| Aporte de: |
| id |
I48-R184-123456789-48511 |
|---|---|
| record_format |
dspace |
| institution |
Universidad Nacional del Nordeste |
| institution_str |
I-48 |
| repository_str |
R-184 |
| collection |
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) |
| language |
Inglés |
| topic |
Biological control Nematophagous fungi Ovicidal activity Geohelminths Control biológico Hongos nematófagos Actividad ovicida Geohelmintos |
| spellingShingle |
Biological control Nematophagous fungi Ovicidal activity Geohelminths Control biológico Hongos nematófagos Actividad ovicida Geohelmintos Bojanich, María Viviana Basualdo, Juan Ángel Giusiano, Gustavo Emilio In vitro effect of chrysosporium indicum and chrysosporium keratinophylum on toxocara canis eggs |
| topic_facet |
Biological control Nematophagous fungi Ovicidal activity Geohelminths Control biológico Hongos nematófagos Actividad ovicida Geohelmintos |
| description |
The degree of antagonism exercised by fungi on geohelminth development varies
according to the morphological alterations caused by different fungal species. Saprophytic fungi
may exert ovicidal or ovistatic effects. The aim of this study was to apply scanning electron
microscopy (SEM) to observe the action of two soil saprophytic species of Chrysosporium (C.
indicum and C. keratinophylum) on Toxocara canis eggs. The fungal strains to be tested were
incubated for 28 days at 28 ◦C in 2% water agar with a suspension of unembryonated T. canis
eggs. A suspension of T. canis eggs in 2% water agar was used as control group. The assay was
done in triplicate for each fungus and the control group. SEM observations were performed
on the 4th, 7th, 14th, 21st, and 28th day after inoculation. The effect of the fungi on eggs
was evaluated in accordance with the alterations observed on the surface and the changes in
the normal characteristics of the eggs. Hyphae around the eggs, appresoria penetrating the
shell and changes in the typical egg membrane were observed in this assay. Type 3 effect
(alterations that occur both in the embryo and the shell, and hyphal penetration of the eggs)
was the prevalent effect. SEM allowed us to observe clearly the morphological alterations in T.
canis eggs due to the effect of C. indicum and C. keratinophylum. Both saprophytic species of
Chrysosporium alter the egg structure and alterations increase as exposure increases. |
| format |
Artículo |
| author |
Bojanich, María Viviana Basualdo, Juan Ángel Giusiano, Gustavo Emilio |
| author_facet |
Bojanich, María Viviana Basualdo, Juan Ángel Giusiano, Gustavo Emilio |
| author_sort |
Bojanich, María Viviana |
| title |
In vitro effect of chrysosporium indicum and chrysosporium keratinophylum on toxocara canis eggs |
| title_short |
In vitro effect of chrysosporium indicum and chrysosporium keratinophylum on toxocara canis eggs |
| title_full |
In vitro effect of chrysosporium indicum and chrysosporium keratinophylum on toxocara canis eggs |
| title_fullStr |
In vitro effect of chrysosporium indicum and chrysosporium keratinophylum on toxocara canis eggs |
| title_full_unstemmed |
In vitro effect of chrysosporium indicum and chrysosporium keratinophylum on toxocara canis eggs |
| title_sort |
in vitro effect of chrysosporium indicum and chrysosporium keratinophylum on toxocara canis eggs |
| publisher |
Asociación Argentina de Microbiología |
| publishDate |
2022 |
| url |
http://repositorio.unne.edu.ar/handle/123456789/48511 |
| work_keys_str_mv |
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| bdutipo_str |
Repositorios |
| _version_ |
1764820539866611713 |