Characterization and expression analysis of somatic embryogenesis receptor kinase (SERK) genes in sexual and apomictic paspalum notatum

The SOMATIC EMBRYOGENESIS RECEPTOR- LIKE KINASE (SERK) gene plays a fundamental role in somatic embryogenesis of angiosperms, and is associated with apomixis in Poa pratensis. The objective of this work was to isolate, characterize and analyze the expression pat- terns of SERK genes in apom...

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Detalles Bibliográficos
Autores principales: Podio, Maricel, Felitti, Silvina Andrea, Siena, Lorena Adelina, Delgado Benarroch, Luciana, Mancini, Micaela, Seijo, José Guillermo, González, Ana María, Pessino, Silvina Claudia, Ortíz, Juan Pablo Amelio
Formato: Artículo
Lenguaje:Inglés
Publicado: Plant Molecular Biology 2022
Materias:
Paspalum notatum
Apomixis
Apospory
SERK
qRT-PCR
In situ hybridization
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/47988
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-47988
record_format dspace
spelling I48-R184-123456789-479882024-09-27T13:43:35Z Characterization and expression analysis of somatic embryogenesis receptor kinase (SERK) genes in sexual and apomictic paspalum notatum Podio, Maricel Felitti, Silvina Andrea Siena, Lorena Adelina Delgado Benarroch, Luciana Mancini, Micaela Seijo, José Guillermo González, Ana María Pessino, Silvina Claudia Ortíz, Juan Pablo Amelio Paspalum notatum Apomixis Apospory SERK qRT-PCR In situ hybridization The SOMATIC EMBRYOGENESIS RECEPTOR- LIKE KINASE (SERK) gene plays a fundamental role in somatic embryogenesis of angiosperms, and is associated with apomixis in Poa pratensis. The objective of this work was to isolate, characterize and analyze the expression pat- terns of SERK genes in apomictic and sexual genotypes of Paspalum notatum. A conserved 200-bp gene fragment was amplified from genomic DNA with heterologous primers, and used to initiate a chromosomal walking strategy for cloning the complete sequence. This procedure allowed the isolation of two members of the P. notatum SERK family; PnSERK1, which is similar to PpSERK1, and PnSERK2, which is similar to ZmSERK2 and AtSERK1. Phylogenetic analyses indicated that PnSERK1 and PnSERK2 represent paralogous sequences. Southern-blot hybridization indi- cated the presence of at least three copies of SERK genes in the species. qRT-PCR analyses revealed that PnSERK2 was expressed at significantly higher levels than PnSERK1 in roots, leaves, reproductive tissues and embryogenic calli.Moreover, in situ hybridization experiments revealed that PnSERK2 displayed a spatially and chronologically altered expression pattern in reproductive organs of the apom- ictic genotype with respect to the sexual one. PnSERK2 is expressed in nucellar cells of the apomictic genotype at meiosis, but only in the megaspore mother cell in the sexual genotype. Therefore, apomixis onset in P. notatum seems to be correlated with the expression of PnSERK2 in nucellar tissue. 2022-06-02T11:43:13Z 2022-06-02T11:43:13Z 2014 Artículo Podio, Maricel, et al., 2014. Characterization and expression analysis of somatic embryogenesis receptor kinase (SERK) genes in sexual and apomictic paspalum notatum. Springer. Ámsterdam: Plant Molecular Biology, vol. 84, no. 4, p. 479-495. ISSN-e 1573-5028. 0167-4412 http://repositorio.unne.edu.ar/handle/123456789/47988 eng https://link.springer.com/article/10.1007/s11103-013-0146-9 https://pubmed.ncbi.nlm.nih.gov/24146222/ openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf application/pdf Plant Molecular Biology Plant Molecular Biology, 2014, vol. 84, no. 4, p. 479-495.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Paspalum notatum
Apomixis
Apospory
SERK
qRT-PCR
In situ hybridization
spellingShingle Paspalum notatum
Apomixis
Apospory
SERK
qRT-PCR
In situ hybridization
Podio, Maricel
Felitti, Silvina Andrea
Siena, Lorena Adelina
Delgado Benarroch, Luciana
Mancini, Micaela
Seijo, José Guillermo
González, Ana María
Pessino, Silvina Claudia
Ortíz, Juan Pablo Amelio
Characterization and expression analysis of somatic embryogenesis receptor kinase (SERK) genes in sexual and apomictic paspalum notatum
topic_facet Paspalum notatum
Apomixis
Apospory
SERK
qRT-PCR
In situ hybridization
description The SOMATIC EMBRYOGENESIS RECEPTOR- LIKE KINASE (SERK) gene plays a fundamental role in somatic embryogenesis of angiosperms, and is associated with apomixis in Poa pratensis. The objective of this work was to isolate, characterize and analyze the expression pat- terns of SERK genes in apomictic and sexual genotypes of Paspalum notatum. A conserved 200-bp gene fragment was amplified from genomic DNA with heterologous primers, and used to initiate a chromosomal walking strategy for cloning the complete sequence. This procedure allowed the isolation of two members of the P. notatum SERK family; PnSERK1, which is similar to PpSERK1, and PnSERK2, which is similar to ZmSERK2 and AtSERK1. Phylogenetic analyses indicated that PnSERK1 and PnSERK2 represent paralogous sequences. Southern-blot hybridization indi- cated the presence of at least three copies of SERK genes in the species. qRT-PCR analyses revealed that PnSERK2 was expressed at significantly higher levels than PnSERK1 in roots, leaves, reproductive tissues and embryogenic calli.Moreover, in situ hybridization experiments revealed that PnSERK2 displayed a spatially and chronologically altered expression pattern in reproductive organs of the apom- ictic genotype with respect to the sexual one. PnSERK2 is expressed in nucellar cells of the apomictic genotype at meiosis, but only in the megaspore mother cell in the sexual genotype. Therefore, apomixis onset in P. notatum seems to be correlated with the expression of PnSERK2 in nucellar tissue.
format Artículo
author Podio, Maricel
Felitti, Silvina Andrea
Siena, Lorena Adelina
Delgado Benarroch, Luciana
Mancini, Micaela
Seijo, José Guillermo
González, Ana María
Pessino, Silvina Claudia
Ortíz, Juan Pablo Amelio
author_facet Podio, Maricel
Felitti, Silvina Andrea
Siena, Lorena Adelina
Delgado Benarroch, Luciana
Mancini, Micaela
Seijo, José Guillermo
González, Ana María
Pessino, Silvina Claudia
Ortíz, Juan Pablo Amelio
author_sort Podio, Maricel
title Characterization and expression analysis of somatic embryogenesis receptor kinase (SERK) genes in sexual and apomictic paspalum notatum
title_short Characterization and expression analysis of somatic embryogenesis receptor kinase (SERK) genes in sexual and apomictic paspalum notatum
title_full Characterization and expression analysis of somatic embryogenesis receptor kinase (SERK) genes in sexual and apomictic paspalum notatum
title_fullStr Characterization and expression analysis of somatic embryogenesis receptor kinase (SERK) genes in sexual and apomictic paspalum notatum
title_full_unstemmed Characterization and expression analysis of somatic embryogenesis receptor kinase (SERK) genes in sexual and apomictic paspalum notatum
title_sort characterization and expression analysis of somatic embryogenesis receptor kinase (serk) genes in sexual and apomictic paspalum notatum
publisher Plant Molecular Biology
publishDate 2022
url http://repositorio.unne.edu.ar/handle/123456789/47988
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