Molecular mechanisms involved in murine bone marrow erythropoietic response to acute anaemia by bleeding

The underlying interactions among apoptosis, eiythroid proliferation and differentiation involved in bone marrow eiythropoietic response after an acute blood-loss have not yet been elucidated in detail. We hypothesized that Erythropoietin receptor, Bax, caspase-3, cytochrome c, Smac/DIABLO and Bcl-x...

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Autores principales: Todaro, Juan Santiago, Stoyanoff, Tania Romina, Aguirre, María Victoria, Brandan, Nora Cristina
Formato: Artículo
Lenguaje:Inglés
Publicado: Science Alert 2022
Materias:
Apoptosis
Bleeding
Bone marrow
Baspase-3-erythropoietin receptor
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/33893
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RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-33893
record_format dspace
spelling I48-R184-123456789-338932024-12-16T11:35:30Z Molecular mechanisms involved in murine bone marrow erythropoietic response to acute anaemia by bleeding Todaro, Juan Santiago Stoyanoff, Tania Romina Aguirre, María Victoria Brandan, Nora Cristina Apoptosis Bleeding Bone marrow Baspase-3-erythropoietin receptor The underlying interactions among apoptosis, eiythroid proliferation and differentiation involved in bone marrow eiythropoietic response after an acute blood-loss have not yet been elucidated in detail. We hypothesized that Erythropoietin receptor, Bax, caspase-3, cytochrome c, Smac/DIABLO and Bcl-xL molecules play important roles at time of acute eiythropoietic need to ameliorate the hypoxic stress. Experiments were performed using in vivo murine model of anaemia induced by blood-loss in a time course study of 15 days. Haematological parameters and bone marrow cellularities were determined. Bone marrow apoptotic assays included: double fluorescent staining (acridine orange/ethidium bromide) and TUNEL. Bone marrow clonogenic assays were performed for evaluating eiythroid colony forming unif s expansión. The Eiythropoietin receptor, Bax, Bcl-xu Smac/DIABLO, caspase-3 and cytochrome c expressions were assessed by immunoblottings. Caspase-3 activity was determined with a colorimetric assay kit. Bleeding induces bone marrow apoptosis from 1 to 3 days, concomitant with Bax over-expression and Bcl-xL decrease. The mitochondrial dysfunction caused cytochrome c and Smac/DIABLO release to cytosol and caspase-3 activation. Eiythropoietic recoveiy was associated with Eiythropoietin receptor over expression from the third day, concomitant with the eiythroid progenitors and Bcl-xL/Bax ratio enhancements. Eiythropoiesis after bleeding depends on a delicate balance among proapoptotic (Bax, caspase-3, cytochrome c, Smac/DIABLO) and prosurvival proteins (Eiythropoietin receptor, Bcl-xL), as the crucial regulators in bone marrow eiythroid recoveiy. These findings provide new insights into the homeostatic mechanisms which promote eiythropoietic response post-bleeding. 2022-04-19T14:42:54Z 2022-04-19T14:42:54Z 2013 Artículo Todaro, Juan Santiago, et al, 2013. Molecular mechanisms involved in murine bone marrow erythropoietic response to acute anaemia by bleeding. Journal of Biological Sciences. New York: Science Alert, vol. 13, no. 6, p. 452-462. ISSN 1812-5719. 1727-3048 http://repositorio.unne.edu.ar/handle/123456789/33893 eng openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf application/pdf Science Alert Journal of Biological Sciences, 2013, vol. 13, no. 6, p. 452-462.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Apoptosis
Bleeding
Bone marrow
Baspase-3-erythropoietin receptor
spellingShingle Apoptosis
Bleeding
Bone marrow
Baspase-3-erythropoietin receptor
Todaro, Juan Santiago
Stoyanoff, Tania Romina
Aguirre, María Victoria
Brandan, Nora Cristina
Molecular mechanisms involved in murine bone marrow erythropoietic response to acute anaemia by bleeding
topic_facet Apoptosis
Bleeding
Bone marrow
Baspase-3-erythropoietin receptor
description The underlying interactions among apoptosis, eiythroid proliferation and differentiation involved in bone marrow eiythropoietic response after an acute blood-loss have not yet been elucidated in detail. We hypothesized that Erythropoietin receptor, Bax, caspase-3, cytochrome c, Smac/DIABLO and Bcl-xL molecules play important roles at time of acute eiythropoietic need to ameliorate the hypoxic stress. Experiments were performed using in vivo murine model of anaemia induced by blood-loss in a time course study of 15 days. Haematological parameters and bone marrow cellularities were determined. Bone marrow apoptotic assays included: double fluorescent staining (acridine orange/ethidium bromide) and TUNEL. Bone marrow clonogenic assays were performed for evaluating eiythroid colony forming unif s expansión. The Eiythropoietin receptor, Bax, Bcl-xu Smac/DIABLO, caspase-3 and cytochrome c expressions were assessed by immunoblottings. Caspase-3 activity was determined with a colorimetric assay kit. Bleeding induces bone marrow apoptosis from 1 to 3 days, concomitant with Bax over-expression and Bcl-xL decrease. The mitochondrial dysfunction caused cytochrome c and Smac/DIABLO release to cytosol and caspase-3 activation. Eiythropoietic recoveiy was associated with Eiythropoietin receptor over expression from the third day, concomitant with the eiythroid progenitors and Bcl-xL/Bax ratio enhancements. Eiythropoiesis after bleeding depends on a delicate balance among proapoptotic (Bax, caspase-3, cytochrome c, Smac/DIABLO) and prosurvival proteins (Eiythropoietin receptor, Bcl-xL), as the crucial regulators in bone marrow eiythroid recoveiy. These findings provide new insights into the homeostatic mechanisms which promote eiythropoietic response post-bleeding.
format Artículo
author Todaro, Juan Santiago
Stoyanoff, Tania Romina
Aguirre, María Victoria
Brandan, Nora Cristina
author_facet Todaro, Juan Santiago
Stoyanoff, Tania Romina
Aguirre, María Victoria
Brandan, Nora Cristina
author_sort Todaro, Juan Santiago
title Molecular mechanisms involved in murine bone marrow erythropoietic response to acute anaemia by bleeding
title_short Molecular mechanisms involved in murine bone marrow erythropoietic response to acute anaemia by bleeding
title_full Molecular mechanisms involved in murine bone marrow erythropoietic response to acute anaemia by bleeding
title_fullStr Molecular mechanisms involved in murine bone marrow erythropoietic response to acute anaemia by bleeding
title_full_unstemmed Molecular mechanisms involved in murine bone marrow erythropoietic response to acute anaemia by bleeding
title_sort molecular mechanisms involved in murine bone marrow erythropoietic response to acute anaemia by bleeding
publisher Science Alert
publishDate 2022
url http://repositorio.unne.edu.ar/handle/123456789/33893
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AT aguirremariavictoria molecularmechanismsinvolvedinmurinebonemarrowerythropoieticresponsetoacuteanaemiabybleeding
AT brandannoracristina molecularmechanismsinvolvedinmurinebonemarrowerythropoieticresponsetoacuteanaemiabybleeding
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