Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in paspalum notatum

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In previous studies we reported the identification of several AFLP, RAPD and RFLP molecular markers linked to apospory in Paspalum notatum. The objective of this work was to sequence these markers, obtain their flanking regions by chromosome walking and perform an in silico mapping analysis in rice...

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Autores principales: Podio, Maricel, Rodríguez, María Pía, Felitti, Silvina Andrea, Stein, Juliana, Martínez, Eric Javier, Siena, Lorena Adelina, Quarin, Camilo Luis, Pessino, Silvina Claudia, Ortíz, Juan Pablo Amelio
Formato: Artículo
Lenguaje:Inglés
Publicado: Sociedade Brasileira de Genética 2022
Materias:
Apomixis
Chromosome walking
Gene mapping
Molecular markers
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/33815
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-33815
record_format dspace
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Apomixis
Chromosome walking
Gene mapping
Molecular markers
spellingShingle Apomixis
Chromosome walking
Gene mapping
Molecular markers
Podio, Maricel
Rodríguez, María Pía
Felitti, Silvina Andrea
Stein, Juliana
Martínez, Eric Javier
Siena, Lorena Adelina
Quarin, Camilo Luis
Pessino, Silvina Claudia
Ortíz, Juan Pablo Amelio
Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in paspalum notatum
topic_facet Apomixis
Chromosome walking
Gene mapping
Molecular markers
description In previous studies we reported the identification of several AFLP, RAPD and RFLP molecular markers linked to apospory in Paspalum notatum. The objective of this work was to sequence these markers, obtain their flanking regions by chromosome walking and perform an in silico mapping analysis in rice and maize. The methylation status of two apospory-related sequences was also assessed using methylation-sensitive RFLP experiments. Fourteen molecular markers were analyzed and several protein-coding sequences were identified. Copy number estimates and RFLP linkage analysis showed that the sequence PnMAI3 displayed 2-4 copies per genome and linkage to apospory. Extension of this marker by chromosome walking revealed an additional protein-coding sequence mapping in silico in the apospory-syntenic regions of rice and maize. Approximately 5 kb corresponding to different markers were characterized through the global sequencing procedure. A more refined analysis based on sequence information indicated synteny with segments of chromosomes 2 and 12 of rice and chromosomes 3 and 5 of maize. Two loci associated with apomixis locus were tested in methylation-sensitive RFLP experiments using genomic DNA extracted from leaves. Although both target sequences were methylated no methylation polymorphisms associated with the mode of reproduction were detected.
format Artículo
author Podio, Maricel
Rodríguez, María Pía
Felitti, Silvina Andrea
Stein, Juliana
Martínez, Eric Javier
Siena, Lorena Adelina
Quarin, Camilo Luis
Pessino, Silvina Claudia
Ortíz, Juan Pablo Amelio
author_facet Podio, Maricel
Rodríguez, María Pía
Felitti, Silvina Andrea
Stein, Juliana
Martínez, Eric Javier
Siena, Lorena Adelina
Quarin, Camilo Luis
Pessino, Silvina Claudia
Ortíz, Juan Pablo Amelio
author_sort Podio, Maricel
title Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in paspalum notatum
title_short Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in paspalum notatum
title_full Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in paspalum notatum
title_fullStr Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in paspalum notatum
title_full_unstemmed Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in paspalum notatum
title_sort sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in paspalum notatum
publisher Sociedade Brasileira de Genética
publishDate 2022
url http://repositorio.unne.edu.ar/handle/123456789/33815
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