Apoptosis induced by a snake venom metalloproteinase from bothrops alternatus venom in C2C12 muscle cells
In this study, the apoptosis inducing effects of baltergin as well as its influence on cell adhesion and migration on muscles cells in vitro were studied. Morphological analysis made by scanning electron and pase contrast microscopy demonstrated typical futures of programmed cell death, apoptosis. T...
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Autores principales: | , , , , |
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Formato: | Artículo |
Lenguaje: | Inglés |
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Springer
2021
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Acceso en línea: | http://repositorio.unne.edu.ar/handle/123456789/30330 |
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I48-R184-123456789-30330 |
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institution |
Universidad Nacional del Nordeste |
institution_str |
I-48 |
repository_str |
R-184 |
collection |
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) |
language |
Inglés |
topic |
Baltergin Metalloproteinase Bothrops alternatus Anoikis Apoptosis |
spellingShingle |
Baltergin Metalloproteinase Bothrops alternatus Anoikis Apoptosis Bustillo, Soledad Van de Velde, Andrea Carolina Matzner Perfumo, Verónica Gay, Claudia Carolina Leiva, Laura Cristina Apoptosis induced by a snake venom metalloproteinase from bothrops alternatus venom in C2C12 muscle cells |
topic_facet |
Baltergin Metalloproteinase Bothrops alternatus Anoikis Apoptosis |
description |
In this study, the apoptosis inducing effects of baltergin as well as its influence on cell adhesion and migration on muscles cells in vitro were studied. Morphological analysis made by scanning electron and pase contrast microscopy demonstrated typical futures of programmed cell death, apoptosis. This mechanism was confirmed by fluorescence staining, molecular analysis of endonuclease activity and increased mRNA expresión level of two representative genes (p53 and bax). On the other hand, baltergin exert an inhibition effect on myoblast cell adhesion and migration in vitro probably through a mechanism that involves the interaction of this enzyme with cell integrins. In conclusion, our results suggest that the absence of appropriate extracellular matrix contacts triggers anoikis. Therefore, this is the first report that demonstrated the mechanism of programmed cell death triggered by baltergin, a PIII metalloprotease isolated from Bothrops alternatus venom, in a myoblast cell line. |
format |
Artículo |
author |
Bustillo, Soledad Van de Velde, Andrea Carolina Matzner Perfumo, Verónica Gay, Claudia Carolina Leiva, Laura Cristina |
author_facet |
Bustillo, Soledad Van de Velde, Andrea Carolina Matzner Perfumo, Verónica Gay, Claudia Carolina Leiva, Laura Cristina |
author_sort |
Bustillo, Soledad |
title |
Apoptosis induced by a snake venom metalloproteinase from bothrops alternatus venom in C2C12 muscle cells |
title_short |
Apoptosis induced by a snake venom metalloproteinase from bothrops alternatus venom in C2C12 muscle cells |
title_full |
Apoptosis induced by a snake venom metalloproteinase from bothrops alternatus venom in C2C12 muscle cells |
title_fullStr |
Apoptosis induced by a snake venom metalloproteinase from bothrops alternatus venom in C2C12 muscle cells |
title_full_unstemmed |
Apoptosis induced by a snake venom metalloproteinase from bothrops alternatus venom in C2C12 muscle cells |
title_sort |
apoptosis induced by a snake venom metalloproteinase from bothrops alternatus venom in c2c12 muscle cells |
publisher |
Springer |
publishDate |
2021 |
url |
http://repositorio.unne.edu.ar/handle/123456789/30330 |
work_keys_str_mv |
AT bustillosoledad apoptosisinducedbyasnakevenommetalloproteinasefrombothropsalternatusvenominc2c12musclecells AT vandeveldeandreacarolina apoptosisinducedbyasnakevenommetalloproteinasefrombothropsalternatusvenominc2c12musclecells AT matznerperfumoveronica apoptosisinducedbyasnakevenommetalloproteinasefrombothropsalternatusvenominc2c12musclecells AT gayclaudiacarolina apoptosisinducedbyasnakevenommetalloproteinasefrombothropsalternatusvenominc2c12musclecells AT leivalauracristina apoptosisinducedbyasnakevenommetalloproteinasefrombothropsalternatusvenominc2c12musclecells |
bdutipo_str |
Repositorios |
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