Apoptosis induced by a snake venom metalloproteinase from bothrops alternatus venom in C2C12 muscle cells

In this study, the apoptosis inducing effects of baltergin as well as its influence on cell adhesion and migration on muscles cells in vitro were studied. Morphological analysis made by scanning electron and pase contrast microscopy demonstrated typical futures of programmed cell death, apoptosis. T...

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Detalles Bibliográficos
Autores principales: Bustillo, Soledad, Van de Velde, Andrea Carolina, Matzner Perfumo, Verónica, Gay, Claudia Carolina, Leiva, Laura Cristina Ana
Formato: Artículo
Lenguaje:Inglés
Publicado: Springer 2021
Materias:
Baltergin
Metalloproteinase
Bothrops alternatus
Anoikis
Apoptosis
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/30330
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-30330
record_format dspace
spelling I48-R184-123456789-303302025-03-06T11:01:57Z Apoptosis induced by a snake venom metalloproteinase from bothrops alternatus venom in C2C12 muscle cells Bustillo, Soledad Van de Velde, Andrea Carolina Matzner Perfumo, Verónica Gay, Claudia Carolina Leiva, Laura Cristina Ana Baltergin Metalloproteinase Bothrops alternatus Anoikis Apoptosis In this study, the apoptosis inducing effects of baltergin as well as its influence on cell adhesion and migration on muscles cells in vitro were studied. Morphological analysis made by scanning electron and pase contrast microscopy demonstrated typical futures of programmed cell death, apoptosis. This mechanism was confirmed by fluorescence staining, molecular analysis of endonuclease activity and increased mRNA expresión level of two representative genes (p53 and bax). On the other hand, baltergin exert an inhibition effect on myoblast cell adhesion and migration in vitro probably through a mechanism that involves the interaction of this enzyme with cell integrins. In conclusion, our results suggest that the absence of appropriate extracellular matrix contacts triggers anoikis. Therefore, this is the first report that demonstrated the mechanism of programmed cell death triggered by baltergin, a PIII metalloprotease isolated from Bothrops alternatus venom, in a myoblast cell line. 2021-12-09T16:27:23Z 2021-12-09T16:27:23Z 2017 Artículo Bustillo, Soledad, et al., 2017. Apoptosis induced by a snake venom metalloproteinase from bothrops alternatus venom in C2C12 muscle cells. Apoptosis. Suiza: Springer, vol. 22, no. 4, p. 491-501.ISSN 1360-8185. 1360-8185 http://repositorio.unne.edu.ar/handle/123456789/30330 eng openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf application/pdf Springer Apoptosis, 2017, vol. 22, no. 4, p. 491-501.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Baltergin
Metalloproteinase
Bothrops alternatus
Anoikis
Apoptosis
spellingShingle Baltergin
Metalloproteinase
Bothrops alternatus
Anoikis
Apoptosis
Bustillo, Soledad
Van de Velde, Andrea Carolina
Matzner Perfumo, Verónica
Gay, Claudia Carolina
Leiva, Laura Cristina Ana
Apoptosis induced by a snake venom metalloproteinase from bothrops alternatus venom in C2C12 muscle cells
topic_facet Baltergin
Metalloproteinase
Bothrops alternatus
Anoikis
Apoptosis
description In this study, the apoptosis inducing effects of baltergin as well as its influence on cell adhesion and migration on muscles cells in vitro were studied. Morphological analysis made by scanning electron and pase contrast microscopy demonstrated typical futures of programmed cell death, apoptosis. This mechanism was confirmed by fluorescence staining, molecular analysis of endonuclease activity and increased mRNA expresión level of two representative genes (p53 and bax). On the other hand, baltergin exert an inhibition effect on myoblast cell adhesion and migration in vitro probably through a mechanism that involves the interaction of this enzyme with cell integrins. In conclusion, our results suggest that the absence of appropriate extracellular matrix contacts triggers anoikis. Therefore, this is the first report that demonstrated the mechanism of programmed cell death triggered by baltergin, a PIII metalloprotease isolated from Bothrops alternatus venom, in a myoblast cell line.
format Artículo
author Bustillo, Soledad
Van de Velde, Andrea Carolina
Matzner Perfumo, Verónica
Gay, Claudia Carolina
Leiva, Laura Cristina Ana
author_facet Bustillo, Soledad
Van de Velde, Andrea Carolina
Matzner Perfumo, Verónica
Gay, Claudia Carolina
Leiva, Laura Cristina Ana
author_sort Bustillo, Soledad
title Apoptosis induced by a snake venom metalloproteinase from bothrops alternatus venom in C2C12 muscle cells
title_short Apoptosis induced by a snake venom metalloproteinase from bothrops alternatus venom in C2C12 muscle cells
title_full Apoptosis induced by a snake venom metalloproteinase from bothrops alternatus venom in C2C12 muscle cells
title_fullStr Apoptosis induced by a snake venom metalloproteinase from bothrops alternatus venom in C2C12 muscle cells
title_full_unstemmed Apoptosis induced by a snake venom metalloproteinase from bothrops alternatus venom in C2C12 muscle cells
title_sort apoptosis induced by a snake venom metalloproteinase from bothrops alternatus venom in c2c12 muscle cells
publisher Springer
publishDate 2021
url http://repositorio.unne.edu.ar/handle/123456789/30330
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