Evidence of the occurrence of structural chromosome changes at the initial diploid diversification of the autopolyploid turnera sidoides l. (passifloraceae) complex

Turnera sidoides is an autopolyploid complex of obligate outcrossing perennial herbs. It includes five subspecies and five morphotypes in which diploid to octoploid cytotypes were found. Based on phenetic analyses of the complex and karyotype data of polyploid cytotypes, it has been hypothesized tha...

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Autores principales: Roggero Luque, Juan Manuel, Moreno, Ercilia María Sara, Kovalsky, Ivana Evelin, Seijo, José Guillermo, Solís Neffa, Viviana Griselda
Formato: Artículo
Lenguaje:Inglés
Publicado: Natl research council Canada-n R C research press 2021
Materias:
Diploid
Chromosome differentiation
Turnera sidoides
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/30299
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-30299
record_format dspace
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Diploid
Chromosome differentiation
Turnera sidoides
spellingShingle Diploid
Chromosome differentiation
Turnera sidoides
Roggero Luque, Juan Manuel
Moreno, Ercilia María Sara
Kovalsky, Ivana Evelin
Seijo, José Guillermo
Solís Neffa, Viviana Griselda
Evidence of the occurrence of structural chromosome changes at the initial diploid diversification of the autopolyploid turnera sidoides l. (passifloraceae) complex
topic_facet Diploid
Chromosome differentiation
Turnera sidoides
description Turnera sidoides is an autopolyploid complex of obligate outcrossing perennial herbs. It includes five subspecies and five morphotypes in which diploid to octoploid cytotypes were found. Based on phenetic analyses of the complex and karyotype data of polyploid cytotypes, it has been hypothesized that morphological and chromosome differentiation of T. sidoides occurred at the diploid level. To test this hypothesis, we present the first detailed chromosome analysis of diploid populations of three subspecies and four morphotypes. CMA+/DAPI− bands were restricted to secondary constrictions (except in the andino morphotype) and varied in number and position among taxa. By contrast, DAPI staining was uniform in all the materials investigated. The number and position of 45S rDNA loci were coincident with the CMA+/DAPI− bands associated with secondary constrictions. Only one pair of 5S rDNA loci was detected in all the taxa (except in subsp. holosericea), but its position was variable. The identified chromosome markers varied among the three subspecies analyzed, but they were more conserved among the morphotypes of subsp. pinnatifida. Cluster analysis of these chromosome markers supports the current taxonomic arrangement of diploids and demonstrates that structural chromosome changes would have led or accompanied the initial differentiation of T. sidoides at the diploid level.
format Artículo
author Roggero Luque, Juan Manuel
Moreno, Ercilia María Sara
Kovalsky, Ivana Evelin
Seijo, José Guillermo
Solís Neffa, Viviana Griselda
author_facet Roggero Luque, Juan Manuel
Moreno, Ercilia María Sara
Kovalsky, Ivana Evelin
Seijo, José Guillermo
Solís Neffa, Viviana Griselda
author_sort Roggero Luque, Juan Manuel
title Evidence of the occurrence of structural chromosome changes at the initial diploid diversification of the autopolyploid turnera sidoides l. (passifloraceae) complex
title_short Evidence of the occurrence of structural chromosome changes at the initial diploid diversification of the autopolyploid turnera sidoides l. (passifloraceae) complex
title_full Evidence of the occurrence of structural chromosome changes at the initial diploid diversification of the autopolyploid turnera sidoides l. (passifloraceae) complex
title_fullStr Evidence of the occurrence of structural chromosome changes at the initial diploid diversification of the autopolyploid turnera sidoides l. (passifloraceae) complex
title_full_unstemmed Evidence of the occurrence of structural chromosome changes at the initial diploid diversification of the autopolyploid turnera sidoides l. (passifloraceae) complex
title_sort evidence of the occurrence of structural chromosome changes at the initial diploid diversification of the autopolyploid turnera sidoides l. (passifloraceae) complex
publisher Natl research council Canada-n R C research press
publishDate 2021
url http://repositorio.unne.edu.ar/handle/123456789/30299
work_keys_str_mv AT roggeroluquejuanmanuel evidenceoftheoccurrenceofstructuralchromosomechangesattheinitialdiploiddiversificationoftheautopolyploidturnerasidoideslpassifloraceaecomplex
AT morenoerciliamariasara evidenceoftheoccurrenceofstructuralchromosomechangesattheinitialdiploiddiversificationoftheautopolyploidturnerasidoideslpassifloraceaecomplex
AT kovalskyivanaevelin evidenceoftheoccurrenceofstructuralchromosomechangesattheinitialdiploiddiversificationoftheautopolyploidturnerasidoideslpassifloraceaecomplex
AT seijojoseguillermo evidenceoftheoccurrenceofstructuralchromosomechangesattheinitialdiploiddiversificationoftheautopolyploidturnerasidoideslpassifloraceaecomplex
AT solisneffavivianagriselda evidenceoftheoccurrenceofstructuralchromosomechangesattheinitialdiploiddiversificationoftheautopolyploidturnerasidoideslpassifloraceaecomplex
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spelling I48-R184-123456789-302992025-10-08T13:29:26Z Evidence of the occurrence of structural chromosome changes at the initial diploid diversification of the autopolyploid turnera sidoides l. (passifloraceae) complex Roggero Luque, Juan Manuel Moreno, Ercilia María Sara Kovalsky, Ivana Evelin Seijo, José Guillermo Solís Neffa, Viviana Griselda Diploid Chromosome differentiation Turnera sidoides Turnera sidoides is an autopolyploid complex of obligate outcrossing perennial herbs. It includes five subspecies and five morphotypes in which diploid to octoploid cytotypes were found. Based on phenetic analyses of the complex and karyotype data of polyploid cytotypes, it has been hypothesized that morphological and chromosome differentiation of T. sidoides occurred at the diploid level. To test this hypothesis, we present the first detailed chromosome analysis of diploid populations of three subspecies and four morphotypes. CMA+/DAPI− bands were restricted to secondary constrictions (except in the andino morphotype) and varied in number and position among taxa. By contrast, DAPI staining was uniform in all the materials investigated. The number and position of 45S rDNA loci were coincident with the CMA+/DAPI− bands associated with secondary constrictions. Only one pair of 5S rDNA loci was detected in all the taxa (except in subsp. holosericea), but its position was variable. The identified chromosome markers varied among the three subspecies analyzed, but they were more conserved among the morphotypes of subsp. pinnatifida. Cluster analysis of these chromosome markers supports the current taxonomic arrangement of diploids and demonstrates that structural chromosome changes would have led or accompanied the initial differentiation of T. sidoides at the diploid level. Le Turnera sidoides représente un complexe d’espèces autopolyploïdes comprenant des plantes pérennes a` allofécondation stricte. Cette espèce compte cinq sous-espèces et cinq types morphologiques au sein desquels des cytotypes diploïdes et octoploïdes ont été rencontrés. Sur la base d’analyses phénétiques au sein de ce complexe et de données caryotypiques chez les cytotypes polyploïdes, il a été proposé que la différenciation morphologique et caryo typique chez le T. sidoides serait survenue a` l’état diploïde. Pour tester cette hypothèse, les auteurs présentent la première analyse chromosomique détaillée de populations diploïdes au sein de trois sous-espèces et de quatre types morphologiques. Les marquages CMA+/DAPI− étaient limités aux constrictions secondaires (sauf au sein du type mor phologique andino) et tant le nombre que la position des bandes étaient variables entre les taxons. Au contraire, le marquage au DAPI était uniforme chez tous les échantillons examinés. Le nombre et la position des locus d’ADNr 45S coïncidaient avec les bandes CMA+/DAPI− associées aux constrictions secondaires. Seule une paire de locus d’ADNr 5S a été détectée chez tous les taxons (sauf chez la sous-espèce holoserica), mais sa position était variable. Les marqueurs permettant l’identification des chromosomes variaient entre les trois sous-espèces étudiées, mais ils étaient davantage conservés au sein des types morphologiques de la sous-espèce pinnatifida. Une analyse de groupement de ces marqueurs chromosomiques amontré que des changements structuraux au sein des chromosomes auraientmené ou accompagné la différenciation initiale au sein du T. sidoides a` l’état diploïde. [Traduit par la Rédaction] 2021-12-07T19:13:37Z 2021-12-07T19:13:37Z 2015 Artículo Roggero Luque, Juan Manuel, et al., 2015. Evidence of the occurrence of structural chromosome changes at the initial diploid diversification of the autopolyploid turnera sidoides l. (passifloraceae) complex. Genome. Otawa: Natl research council Canada-n R C research press, vol. 59, p. 1-10. ISSN 0831-2796. 0831-2796 http://repositorio.unne.edu.ar/handle/123456789/30299 eng openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf application/pdf Natl research council Canada-n R C research press Genome, 2015, vol. 59, p. 1-10.

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