Characterization of brazilian accessions of wild arachis species of section arachis (fabaceae) using heterochromatin detection and fluorescence in situ hybridization (FISH)

The cytogenetic characterization of Arachis species is useful for assessing the genomes present in this genus, for establishing the relationship among their representatives and for understanding the variability in the available germplasm. In this study, we used fluorescence in situ hybridization (FI...

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Autores principales: Custodio, Adriana Regina, Seijo, José Guillermo, Montenegro Valls, José Francisco
Formato: Artículo
Lenguaje:Inglés
Publicado: Sociedade Brasileira de Genética 2021
Materias:
Arachis
Chromosome
Genomes
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/28897
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-28897
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spelling I48-R184-123456789-288972024-09-27T13:58:14Z Characterization of brazilian accessions of wild arachis species of section arachis (fabaceae) using heterochromatin detection and fluorescence in situ hybridization (FISH) Custodio, Adriana Regina Seijo, José Guillermo Montenegro Valls, José Francisco Arachis Chromosome Genomes The cytogenetic characterization of Arachis species is useful for assessing the genomes present in this genus, for establishing the relationship among their representatives and for understanding the variability in the available germplasm. In this study, we used fluorescence in situ hybridization (FISH) to examine the distribution patterns of heterochromatin and rDNA genes in 12 Brazilian accessions of five species of the taxonomic section Arachis. The heterochromatic pattern varied considerably among the species: complements with centromeric bands in all of the chromosomes (A. hoehnei) and complements completely devoid of heterochromatin (A. gregoryi, A. magna) were observed. The number of 45S rDNA loci ranged from two (A. gregoryi) to eight (A. glandulifera), while the number of 5S rDNA loci was more conserved and varied from two (in most species) to four (A. hoehnei). In some species one pair of 5S rDNA loci was observed adjacent to 45S rDNA loci. The chromosomal markers revealed polymorphism in the three species with more than one accession (A. gregoryi, A. magna and A. valida) that were tested. The previous genome assignment for each of the species studied was confirmed, except for A. hoehnei. The intraspecific variability observed here suggests that an exhaustive cytogenetic and taxonomic analysis is still needed for some Arachis species. 2021-10-04T15:56:19Z 2021-10-04T15:56:19Z 2013 Artículo Custodio, Adriana Regina, Seijo, José Guillermo y Montenegro Valls, José Francisco, 2013. Characterization of brazilian accessions of wild arachis species of section arachis (fabaceae) using heterochromatin detection and fluorescence in situ hybridization (FISH). Genetics And Molecular Biology. Ribeirão Preto: Sociedade Brasileira de Genética, vol. 36, no. 3, p. 364-370. ISSN 2027-3355. 1678-4685 http://repositorio.unne.edu.ar/handle/123456789/28897 eng https://www.scielo.br/j/gmb/a/Drtw59SWjBf5Fjs6XhSbttf/?format=pdf&lang=en https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3795162/ openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf application/pdf Sociedade Brasileira de Genética Genetics And Molecular Biology, 2013, vol. 36, no. 3, p. 364-370.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Arachis
Chromosome
Genomes
spellingShingle Arachis
Chromosome
Genomes
Custodio, Adriana Regina
Seijo, José Guillermo
Montenegro Valls, José Francisco
Characterization of brazilian accessions of wild arachis species of section arachis (fabaceae) using heterochromatin detection and fluorescence in situ hybridization (FISH)
topic_facet Arachis
Chromosome
Genomes
description The cytogenetic characterization of Arachis species is useful for assessing the genomes present in this genus, for establishing the relationship among their representatives and for understanding the variability in the available germplasm. In this study, we used fluorescence in situ hybridization (FISH) to examine the distribution patterns of heterochromatin and rDNA genes in 12 Brazilian accessions of five species of the taxonomic section Arachis. The heterochromatic pattern varied considerably among the species: complements with centromeric bands in all of the chromosomes (A. hoehnei) and complements completely devoid of heterochromatin (A. gregoryi, A. magna) were observed. The number of 45S rDNA loci ranged from two (A. gregoryi) to eight (A. glandulifera), while the number of 5S rDNA loci was more conserved and varied from two (in most species) to four (A. hoehnei). In some species one pair of 5S rDNA loci was observed adjacent to 45S rDNA loci. The chromosomal markers revealed polymorphism in the three species with more than one accession (A. gregoryi, A. magna and A. valida) that were tested. The previous genome assignment for each of the species studied was confirmed, except for A. hoehnei. The intraspecific variability observed here suggests that an exhaustive cytogenetic and taxonomic analysis is still needed for some Arachis species.
format Artículo
author Custodio, Adriana Regina
Seijo, José Guillermo
Montenegro Valls, José Francisco
author_facet Custodio, Adriana Regina
Seijo, José Guillermo
Montenegro Valls, José Francisco
author_sort Custodio, Adriana Regina
title Characterization of brazilian accessions of wild arachis species of section arachis (fabaceae) using heterochromatin detection and fluorescence in situ hybridization (FISH)
title_short Characterization of brazilian accessions of wild arachis species of section arachis (fabaceae) using heterochromatin detection and fluorescence in situ hybridization (FISH)
title_full Characterization of brazilian accessions of wild arachis species of section arachis (fabaceae) using heterochromatin detection and fluorescence in situ hybridization (FISH)
title_fullStr Characterization of brazilian accessions of wild arachis species of section arachis (fabaceae) using heterochromatin detection and fluorescence in situ hybridization (FISH)
title_full_unstemmed Characterization of brazilian accessions of wild arachis species of section arachis (fabaceae) using heterochromatin detection and fluorescence in situ hybridization (FISH)
title_sort characterization of brazilian accessions of wild arachis species of section arachis (fabaceae) using heterochromatin detection and fluorescence in situ hybridization (fish)
publisher Sociedade Brasileira de Genética
publishDate 2021
url http://repositorio.unne.edu.ar/handle/123456789/28897
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AT seijojoseguillermo characterizationofbrazilianaccessionsofwildarachisspeciesofsectionarachisfabaceaeusingheterochromatindetectionandfluorescenceinsituhybridizationfish
AT montenegrovallsjosefrancisco characterizationofbrazilianaccessionsofwildarachisspeciesofsectionarachisfabaceaeusingheterochromatindetectionandfluorescenceinsituhybridizationfish
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