Transference of eheat expressed sequence tag-simple sequence repeats to paspalum species and cross-species amplification of paspalum notatum simple sequence repeats : potential use in phylogenetic analysis and mapping

The genus Paspalum L. includes numerous species of agronomic importance. The objectives of this work were to evaluate the transferability and polymorphism of publicly available wheat (Triticum aestivum L.) expressed sequence tag (EST)–simple sequence repeat (SSR) (wEST-SSR) markers to Paspalum spp.,...

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Detalles Bibliográficos
Autores principales: Siena, Lorena Adelina, Sartor, María Esperanza, Quarin, Camilo Luis, Espinoza, Francisco, Ortíz, Juan Pablo Amelio
Formato: Artículo
Lenguaje:Inglés
Publicado: Crop Science Society of America 2021
Materias:
Paspalum
Species
Analysis and mapping
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/28622
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-28622
record_format dspace
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Paspalum
Species
Analysis and mapping
spellingShingle Paspalum
Species
Analysis and mapping
Siena, Lorena Adelina
Sartor, María Esperanza
Quarin, Camilo Luis
Espinoza, Francisco
Ortíz, Juan Pablo Amelio
Transference of eheat expressed sequence tag-simple sequence repeats to paspalum species and cross-species amplification of paspalum notatum simple sequence repeats : potential use in phylogenetic analysis and mapping
topic_facet Paspalum
Species
Analysis and mapping
description The genus Paspalum L. includes numerous species of agronomic importance. The objectives of this work were to evaluate the transferability and polymorphism of publicly available wheat (Triticum aestivum L.) expressed sequence tag (EST)–simple sequence repeat (SSR) (wEST-SSR) markers to Paspalum spp., assess the cross-species amplification of Paspalum notatum Flüggé genomic SSRs (PnSSRs) within the genus, and evaluate both types of markers for phylogenetic analyses and mapping. Thirty-two accessions including 10 species were used. Moreover, 65 F1 hybrids of P. notatum were used for mapping. Transferability ratio of wEST-SSRs was 72.72%. On average 19.25 bands per primer combination were obtained. Cross-species amplification of PnSSRs was 55.37%, with an average of 9.45 fragments per primer pair. Both types of markers differed in the amplification capacity between primers pairs and species. Clustering analysis with wEST-SSR data discriminated accessions by species and taxonomic groups. Genomic relationships were in agreement with previous works indicating that wEST-SSRs are adequate for phylogenetic surveys in the genus. Mapping experiments showed that both wEST-SSRs and PnSSRs mapped scattered in the genome. One hundred two new markers were added to the existent P. notatum linkage groups. Primer pairs ksum206, ksum219, and PN03 F2 generated markers linked to apospory. Sequences of EST-SSRs experimentally mapped in P. notatum showed 46.23 to 55.27% similarity with the original wheat EST. A preliminary comparative mapping analysis was carried combining experimental and in silico mapping results.
format Artículo
author Siena, Lorena Adelina
Sartor, María Esperanza
Quarin, Camilo Luis
Espinoza, Francisco
Ortíz, Juan Pablo Amelio
author_facet Siena, Lorena Adelina
Sartor, María Esperanza
Quarin, Camilo Luis
Espinoza, Francisco
Ortíz, Juan Pablo Amelio
author_sort Siena, Lorena Adelina
title Transference of eheat expressed sequence tag-simple sequence repeats to paspalum species and cross-species amplification of paspalum notatum simple sequence repeats : potential use in phylogenetic analysis and mapping
title_short Transference of eheat expressed sequence tag-simple sequence repeats to paspalum species and cross-species amplification of paspalum notatum simple sequence repeats : potential use in phylogenetic analysis and mapping
title_full Transference of eheat expressed sequence tag-simple sequence repeats to paspalum species and cross-species amplification of paspalum notatum simple sequence repeats : potential use in phylogenetic analysis and mapping
title_fullStr Transference of eheat expressed sequence tag-simple sequence repeats to paspalum species and cross-species amplification of paspalum notatum simple sequence repeats : potential use in phylogenetic analysis and mapping
title_full_unstemmed Transference of eheat expressed sequence tag-simple sequence repeats to paspalum species and cross-species amplification of paspalum notatum simple sequence repeats : potential use in phylogenetic analysis and mapping
title_sort transference of eheat expressed sequence tag-simple sequence repeats to paspalum species and cross-species amplification of paspalum notatum simple sequence repeats : potential use in phylogenetic analysis and mapping
publisher Crop Science Society of America
publishDate 2021
url http://repositorio.unne.edu.ar/handle/123456789/28622
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