Identification of Genes Related to Endosperm Balance Number Insensitivity in Paspalum notatum

The endosperm balance number (EBN) theory states that genomic contribution ratio should be kept at maternal/paternal (m:p) ratio for normal endosperm development. However, the endosperm formation in apomictic Paspalum notatum Flüggé does not depend on the EBN. The aim of this work was to characteriz...

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Autores principales: Depetris, Mara Belén, Acuña, Carlos Alberto, Pozzi, Florencia Ileana, Quarin, Camilo Luis, Felitti, Silvina Andrea
Formato: Artículo
Lenguaje:Inglés
Publicado: Crop Science Society of America 2021
Materias:
Apominix
Emdosperm
Paspalum
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/28610
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-28610
record_format dspace
spelling I48-R184-123456789-286102025-08-06T17:58:05Z Identification of Genes Related to Endosperm Balance Number Insensitivity in Paspalum notatum Depetris, Mara Belén Acuña, Carlos Alberto Pozzi, Florencia Ileana Quarin, Camilo Luis Felitti, Silvina Andrea Apominix Emdosperm Paspalum The endosperm balance number (EBN) theory states that genomic contribution ratio should be kept at maternal/paternal (m:p) ratio for normal endosperm development. However, the endosperm formation in apomictic Paspalum notatum Flüggé does not depend on the EBN. The aim of this work was to characterize gene expression during seed formation from apomictic and sexual P. notatum using complementary DNA-amplified fragment length polymorphism methodology. To induce the formation of seeds with different m:p genomic ratios, crosses were made between genotypes with different ploidy levels and reproductive modes. RNA was isolated from ovaries 24 h after pollination, when maximum endosperm growth rate was expected. Some of the 49 differentially expressed transcript-derived fragments (DETDFs) provided relevant information. Three of those were found in ovaries of apomictic plants with an EBN different from. A DETDF was predicted to be involved in sucrose metabolism during the accumulation of hexose and starch in the endosperm and might be related to EBN insensitivity. Twenty-two DETDFs were found in crosses where sexual plants were used as the female parent and the predicted m:p ratio was not. One of those was related to the failure of fusion of the polar nuclei in Arabidopsis thaliana (L.) Heynh. Finally, three transcripts presented similarity with a casein kinase II that regulates the accumulation of storage products in seeds of A. thaliana. Both processes might be involved in endosperm development in P. notatum. 2021-09-09T14:43:09Z 2021-09-09T14:43:09Z 2018-02 Artículo Depetris, Mara Belén, et al., 2018. Identification of Genes Related to Endosperm Balance Number Insensitivity in Paspalum notatum. Crop Science. Madison: Crop Science Society of America, vol. 58, no. 2, p. 813-822. ISSN 1435-0653. 0011-183X http://repositorio.unne.edu.ar/handle/123456789/28610 eng https://doi.org/10.2135/cropsci2017.04.0260 openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 813- 822 application/pdf Crop Science Society of America Crop Science, vol. 58, no. 2, p. 813- 822.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Apominix
Emdosperm
Paspalum
spellingShingle Apominix
Emdosperm
Paspalum
Depetris, Mara Belén
Acuña, Carlos Alberto
Pozzi, Florencia Ileana
Quarin, Camilo Luis
Felitti, Silvina Andrea
Identification of Genes Related to Endosperm Balance Number Insensitivity in Paspalum notatum
topic_facet Apominix
Emdosperm
Paspalum
description The endosperm balance number (EBN) theory states that genomic contribution ratio should be kept at maternal/paternal (m:p) ratio for normal endosperm development. However, the endosperm formation in apomictic Paspalum notatum Flüggé does not depend on the EBN. The aim of this work was to characterize gene expression during seed formation from apomictic and sexual P. notatum using complementary DNA-amplified fragment length polymorphism methodology. To induce the formation of seeds with different m:p genomic ratios, crosses were made between genotypes with different ploidy levels and reproductive modes. RNA was isolated from ovaries 24 h after pollination, when maximum endosperm growth rate was expected. Some of the 49 differentially expressed transcript-derived fragments (DETDFs) provided relevant information. Three of those were found in ovaries of apomictic plants with an EBN different from. A DETDF was predicted to be involved in sucrose metabolism during the accumulation of hexose and starch in the endosperm and might be related to EBN insensitivity. Twenty-two DETDFs were found in crosses where sexual plants were used as the female parent and the predicted m:p ratio was not. One of those was related to the failure of fusion of the polar nuclei in Arabidopsis thaliana (L.) Heynh. Finally, three transcripts presented similarity with a casein kinase II that regulates the accumulation of storage products in seeds of A. thaliana. Both processes might be involved in endosperm development in P. notatum.
format Artículo
author Depetris, Mara Belén
Acuña, Carlos Alberto
Pozzi, Florencia Ileana
Quarin, Camilo Luis
Felitti, Silvina Andrea
author_facet Depetris, Mara Belén
Acuña, Carlos Alberto
Pozzi, Florencia Ileana
Quarin, Camilo Luis
Felitti, Silvina Andrea
author_sort Depetris, Mara Belén
title Identification of Genes Related to Endosperm Balance Number Insensitivity in Paspalum notatum
title_short Identification of Genes Related to Endosperm Balance Number Insensitivity in Paspalum notatum
title_full Identification of Genes Related to Endosperm Balance Number Insensitivity in Paspalum notatum
title_fullStr Identification of Genes Related to Endosperm Balance Number Insensitivity in Paspalum notatum
title_full_unstemmed Identification of Genes Related to Endosperm Balance Number Insensitivity in Paspalum notatum
title_sort identification of genes related to endosperm balance number insensitivity in paspalum notatum
publisher Crop Science Society of America
publishDate 2021
url http://repositorio.unne.edu.ar/handle/123456789/28610
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AT quarincamiloluis identificationofgenesrelatedtoendospermbalancenumberinsensitivityinpaspalumnotatum
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