Differential auxin transport and accumulation in the stem base lead to profuse adventitious root primordia formation in the aerial roots (aer) mutant of tomato (Solanum lycopersicum L.)

The aerial roots (aer) mutant of tomato is characterized by a profuse and precocious formation of adven- titious root primordia along the stem. We demonstrated that auxin is involved in the aer phenotype but ruled out higher auxin sensitivity of mutant plants. Interestingly, polar auxin transpor...

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Autores principales:	Mignolli, Francesco, Mariotti, Lorenzo, Picciarelli, Piero, Vidoz, María Laura
Formato:	Artículo
Lenguaje:	Inglés
Publicado:	Elsevier GmbH 2021
Materias:	Tomato Aerial roots mutant Adventitious roots
Acceso en línea:	http://repositorio.unne.edu.ar/handle/123456789/28508 https://www.sciencedirect.com/science/article/abs/pii/S0176161717300603
Aporte de:	RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste

id	I48-R184-123456789-28508
record_format	dspace
spelling	I48-R184-123456789-285082024-09-30T13:02:28Z Differential auxin transport and accumulation in the stem base lead to profuse adventitious root primordia formation in the aerial roots (aer) mutant of tomato (Solanum lycopersicum L.) Mignolli, Francesco Mariotti, Lorenzo Picciarelli, Piero Vidoz, María Laura Tomato Aerial roots mutant Adventitious roots The aerial roots (aer) mutant of tomato is characterized by a profuse and precocious formation of adven- titious root primordia along the stem. We demonstrated that auxin is involved in the aer phenotype but ruled out higher auxin sensitivity of mutant plants. Interestingly, polar auxin transport was altered in aer, as young seedlings showed a reduced response to an auxin transport inhibitor and higher expression of auxin export carriers SlPIN1 and SlPIN3. An abrupt reduction in transcripts of auxin efflux and influx genes in older aer hypocotyls caused a marked deceleration of auxin transport in more mature tissues. Indeed, in 20 days old aer plants, the transport of labeled IAA was faster in apices than in hypocotyls, displaying an opposite trend in comparison to a wild type. In addition, auxin transport facilitators (SlPIN1, SlPIN4, SlLAX5) were more expressed in aer apices than in hypocotyls, suggesting that auxin moves faster from the upper to the lower part of the stem. Consequently, a significantly higher level of free and conjugated IAA was found at the base of aer stems with respect to their apices. This auxin accumulation is likely the cause of the aer phenotype. 2021-08-30T14:54:08Z 2021-08-30T14:54:08Z 2017-06 Artículo Mignolli, Francesco, et al., 2017. Differential auxin transport and accumulation in the stem base lead to profuse adventitious root primordia formation in the aerial roots (aer) mutant of tomato (Solanum lycopersicum L.). Journal of Plant Physiology. Múnich: Elsevier GmbH, vol. 213, p. 55-65. E-ISSN 0176-1617. http://repositorio.unne.edu.ar/handle/123456789/28508 https://www.sciencedirect.com/science/article/abs/pii/S0176161717300603 eng openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf application/pdf Elsevier GmbH Journal of Plant Physiology, 2017, vol. 213, p. 55-65.
institution	Universidad Nacional del Nordeste
institution_str	I-48
repository_str	R-184
collection	RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language	Inglés
topic	Tomato Aerial roots mutant Adventitious roots
spellingShingle	Tomato Aerial roots mutant Adventitious roots Mignolli, Francesco Mariotti, Lorenzo Picciarelli, Piero Vidoz, María Laura Differential auxin transport and accumulation in the stem base lead to profuse adventitious root primordia formation in the aerial roots (aer) mutant of tomato (Solanum lycopersicum L.)
topic_facet	Tomato Aerial roots mutant Adventitious roots
description	The aerial roots (aer) mutant of tomato is characterized by a profuse and precocious formation of adven- titious root primordia along the stem. We demonstrated that auxin is involved in the aer phenotype but ruled out higher auxin sensitivity of mutant plants. Interestingly, polar auxin transport was altered in aer, as young seedlings showed a reduced response to an auxin transport inhibitor and higher expression of auxin export carriers SlPIN1 and SlPIN3. An abrupt reduction in transcripts of auxin efflux and influx genes in older aer hypocotyls caused a marked deceleration of auxin transport in more mature tissues. Indeed, in 20 days old aer plants, the transport of labeled IAA was faster in apices than in hypocotyls, displaying an opposite trend in comparison to a wild type. In addition, auxin transport facilitators (SlPIN1, SlPIN4, SlLAX5) were more expressed in aer apices than in hypocotyls, suggesting that auxin moves faster from the upper to the lower part of the stem. Consequently, a significantly higher level of free and conjugated IAA was found at the base of aer stems with respect to their apices. This auxin accumulation is likely the cause of the aer phenotype.
format	Artículo
author	Mignolli, Francesco Mariotti, Lorenzo Picciarelli, Piero Vidoz, María Laura
author_facet	Mignolli, Francesco Mariotti, Lorenzo Picciarelli, Piero Vidoz, María Laura
author_sort	Mignolli, Francesco
title	Differential auxin transport and accumulation in the stem base lead to profuse adventitious root primordia formation in the aerial roots (aer) mutant of tomato (Solanum lycopersicum L.)
title_short	Differential auxin transport and accumulation in the stem base lead to profuse adventitious root primordia formation in the aerial roots (aer) mutant of tomato (Solanum lycopersicum L.)
title_full	Differential auxin transport and accumulation in the stem base lead to profuse adventitious root primordia formation in the aerial roots (aer) mutant of tomato (Solanum lycopersicum L.)
title_fullStr	Differential auxin transport and accumulation in the stem base lead to profuse adventitious root primordia formation in the aerial roots (aer) mutant of tomato (Solanum lycopersicum L.)
title_full_unstemmed	Differential auxin transport and accumulation in the stem base lead to profuse adventitious root primordia formation in the aerial roots (aer) mutant of tomato (Solanum lycopersicum L.)
title_sort	differential auxin transport and accumulation in the stem base lead to profuse adventitious root primordia formation in the aerial roots (aer) mutant of tomato (solanum lycopersicum l.)
publisher	Elsevier GmbH
publishDate	2021
url	http://repositorio.unne.edu.ar/handle/123456789/28508 https://www.sciencedirect.com/science/article/abs/pii/S0176161717300603
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Differential auxin transport and accumulation in the stem base lead to profuse adventitious root primordia formation in the aerial roots (aer) mutant of tomato (Solanum lycopersicum L.)

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