Induction of gibberellin 20-oxidases and repression of gibberellin 2b-oxidases in unfertilized ovaries of entire tomato mutant, leads to accumulation of active gibberellins and parthenocarpic fruit formation

In tomato (Solanum lycopersicum L.), auxin and gibberellins (GAs) cross-talk plays an important role during fruit-set. The entire tomato mutant has been previ- ously reported to carry a deletion in the coding region of the SlIAA9 gene, a member of the auxin signal repressor family Aux/IAA....

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Mignolli, Francesco, Vidoz, María Laura, Mariotti, Lorenzo, Lombardi, Lara, Picciarelli, Piero
Formato: Artículo
Lenguaje:Inglés
Publicado: Springer 2021
Materias:
Entire mutant
Fruit development
Parthenocarpy
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/28499
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-28499
record_format dspace
spelling I48-R184-123456789-284992024-10-09T14:12:09Z Induction of gibberellin 20-oxidases and repression of gibberellin 2b-oxidases in unfertilized ovaries of entire tomato mutant, leads to accumulation of active gibberellins and parthenocarpic fruit formation Mignolli, Francesco Vidoz, María Laura Mariotti, Lorenzo Lombardi, Lara Picciarelli, Piero Entire mutant Fruit development Parthenocarpy In tomato (Solanum lycopersicum L.), auxin and gibberellins (GAs) cross-talk plays an important role during fruit-set. The entire tomato mutant has been previ- ously reported to carry a deletion in the coding region of the SlIAA9 gene, a member of the auxin signal repressor family Aux/IAA. In this paper, we examined the role of ENTIRE gene in controlling GAs metabolism and directing spontaneous fruit initiation and early ovary growth. It was shown that, similarly to pollinated fruits, facultative par- thenocarpy in entire depends on active GA metabolism, since fruit growth is suppressed when GA biosynthesis is blocked. Analysis of endogenous GAs during the first 10 days after flower emasculation revealed that entire fruits accumulated higher amounts of active GAs (GA1 and GA3) in comparison to wild type pollinated fruits, sug- gesting that a different GA homeostasis regulation occurs. Transcript analysis of the main GA biosynthesis genes showed that differently from unpollinated and non par- thenocarpic wild type ovaries, in entire active GA flux modulation is regulated by the activation of SlGA20ox1 and SlGA20ox2 and also by a marked reduction of GA catabolism (reduced transcription of GA 2b-oxidase genes) during the early fruit expansion phase. 2021-08-30T13:40:33Z 2021-08-30T13:40:33Z 2015-03 Artículo Mignolli, Francesco, et al., 2015. Induction of gibberellin 20-oxidases and repression of gibberellin 2b-oxidases in unfertilized ovaries of entire tomato mutant, leads to accumulation of active gibberellins and parthenocarpic fruit formation. Plant Growth Regulation. Basel: Springer, vol. 75, no. 2, p. 415-425. ISSN 1573-5087. 0167-6903 http://repositorio.unne.edu.ar/handle/123456789/28499 eng https://link.springer.com/article/10.1007/s10725-014-0002-1 openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf application/pdf Springer Plant Growth Regulation, 2015, vol. 75, no. 2, p. 415-425.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Entire mutant
Fruit development
Parthenocarpy
spellingShingle Entire mutant
Fruit development
Parthenocarpy
Mignolli, Francesco
Vidoz, María Laura
Mariotti, Lorenzo
Lombardi, Lara
Picciarelli, Piero
Induction of gibberellin 20-oxidases and repression of gibberellin 2b-oxidases in unfertilized ovaries of entire tomato mutant, leads to accumulation of active gibberellins and parthenocarpic fruit formation
topic_facet Entire mutant
Fruit development
Parthenocarpy
description In tomato (Solanum lycopersicum L.), auxin and gibberellins (GAs) cross-talk plays an important role during fruit-set. The entire tomato mutant has been previ- ously reported to carry a deletion in the coding region of the SlIAA9 gene, a member of the auxin signal repressor family Aux/IAA. In this paper, we examined the role of ENTIRE gene in controlling GAs metabolism and directing spontaneous fruit initiation and early ovary growth. It was shown that, similarly to pollinated fruits, facultative par- thenocarpy in entire depends on active GA metabolism, since fruit growth is suppressed when GA biosynthesis is blocked. Analysis of endogenous GAs during the first 10 days after flower emasculation revealed that entire fruits accumulated higher amounts of active GAs (GA1 and GA3) in comparison to wild type pollinated fruits, sug- gesting that a different GA homeostasis regulation occurs. Transcript analysis of the main GA biosynthesis genes showed that differently from unpollinated and non par- thenocarpic wild type ovaries, in entire active GA flux modulation is regulated by the activation of SlGA20ox1 and SlGA20ox2 and also by a marked reduction of GA catabolism (reduced transcription of GA 2b-oxidase genes) during the early fruit expansion phase.
format Artículo
author Mignolli, Francesco
Vidoz, María Laura
Mariotti, Lorenzo
Lombardi, Lara
Picciarelli, Piero
author_facet Mignolli, Francesco
Vidoz, María Laura
Mariotti, Lorenzo
Lombardi, Lara
Picciarelli, Piero
author_sort Mignolli, Francesco
title Induction of gibberellin 20-oxidases and repression of gibberellin 2b-oxidases in unfertilized ovaries of entire tomato mutant, leads to accumulation of active gibberellins and parthenocarpic fruit formation
title_short Induction of gibberellin 20-oxidases and repression of gibberellin 2b-oxidases in unfertilized ovaries of entire tomato mutant, leads to accumulation of active gibberellins and parthenocarpic fruit formation
title_full Induction of gibberellin 20-oxidases and repression of gibberellin 2b-oxidases in unfertilized ovaries of entire tomato mutant, leads to accumulation of active gibberellins and parthenocarpic fruit formation
title_fullStr Induction of gibberellin 20-oxidases and repression of gibberellin 2b-oxidases in unfertilized ovaries of entire tomato mutant, leads to accumulation of active gibberellins and parthenocarpic fruit formation
title_full_unstemmed Induction of gibberellin 20-oxidases and repression of gibberellin 2b-oxidases in unfertilized ovaries of entire tomato mutant, leads to accumulation of active gibberellins and parthenocarpic fruit formation
title_sort induction of gibberellin 20-oxidases and repression of gibberellin 2b-oxidases in unfertilized ovaries of entire tomato mutant, leads to accumulation of active gibberellins and parthenocarpic fruit formation
publisher Springer
publishDate 2021
url http://repositorio.unne.edu.ar/handle/123456789/28499
work_keys_str_mv AT mignollifrancesco inductionofgibberellin20oxidasesandrepressionofgibberellin2boxidasesinunfertilizedovariesofentiretomatomutantleadstoaccumulationofactivegibberellinsandparthenocarpicfruitformation
AT vidozmarialaura inductionofgibberellin20oxidasesandrepressionofgibberellin2boxidasesinunfertilizedovariesofentiretomatomutantleadstoaccumulationofactivegibberellinsandparthenocarpicfruitformation
AT mariottilorenzo inductionofgibberellin20oxidasesandrepressionofgibberellin2boxidasesinunfertilizedovariesofentiretomatomutantleadstoaccumulationofactivegibberellinsandparthenocarpicfruitformation
AT lombardilara inductionofgibberellin20oxidasesandrepressionofgibberellin2boxidasesinunfertilizedovariesofentiretomatomutantleadstoaccumulationofactivegibberellinsandparthenocarpicfruitformation
AT picciarellipiero inductionofgibberellin20oxidasesandrepressionofgibberellin2boxidasesinunfertilizedovariesofentiretomatomutantleadstoaccumulationofactivegibberellinsandparthenocarpicfruitformation
_version_ 1832344815297101824

Ejemplares similares