Effects of chlorpyrifos on the growth and ultrastructure of green algae, ankistrodesmus gracilis

The effect of the organophosphorus insecticide chlorpyrifos on the growth, biovolume, and ultrastructure of the green microalga Ankistrodesmus gracilis was evaluated. Concentrations of 9.37, 18.75, 37.5, 75 and 150 mg L 1 of chlorpyrifos were assayed along with a control culture. At the end of the b...

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Autores principales:	Asselborn, Viviana Magdalena, Fernández, Carolina, Zalocar de Domitrovic, Yolanda, Parodi, Elisa Rosalía
Formato:	Artículo
Lenguaje:	Inglés
Publicado:	Elsevier 2021
Materias:	Pesticide Microalgae Toxicity Ultrastructure
Acceso en línea:	http://repositorio.unne.edu.ar/handle/123456789/28489
Aporte de:	RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste

id	I48-R184-123456789-28489
record_format	dspace
spelling	I48-R184-123456789-284892025-03-06T11:01:07Z Effects of chlorpyrifos on the growth and ultrastructure of green algae, ankistrodesmus gracilis Asselborn, Viviana Magdalena Fernández, Carolina Zalocar de Domitrovic, Yolanda Parodi, Elisa Rosalía Pesticide Microalgae Toxicity Ultrastructure The effect of the organophosphorus insecticide chlorpyrifos on the growth, biovolume, and ultrastructure of the green microalga Ankistrodesmus gracilis was evaluated. Concentrations of 9.37, 18.75, 37.5, 75 and 150 mg L 1 of chlorpyrifos were assayed along with a control culture. At the end of the bioassay the ultrastructure of algal cells from control culture and from cultures exposed to 37.5 and 150 mg L 1 was observed under transmission (TEM) and scanning electron microscopy (SEM). After 24 and 48 h, treatments with 75 and 150 mg L 1 inhibited the growth of A. gracilis; whereas after 72 and 96 h, all the treatments except at 9.37 mg L 1 significantly affected the algae growth. The effective concentration 50 (EC50) after 96 h was 22.44 mg L 1 of chlorpyrifos. After the exposure to the insecticide, an increase in the biovolume was observed, with a larger increase in cells exposed to 75 and 150 mg L 1 . Radical changes were observed in the ultrastructure of cells exposed to chlorpyrifos. The insecticide affected the cell shape and the distribution of the crests in the wall. At 37.5 mg L 1 electodense bodies were observed along with an increase in the size and number of starch granules. At 150 mg L 1 such bodies occupied almost the whole cytoplasm together with lipids and remains of thylakoids. Autospores formation occurred normally at 37.5 mg L 1 while at 150 mg L 1 karyokinesis occurred, but cell-separation-phase was inhibited. The present study demonstrates that the exposure of phytoplankton to the insecticide chlorpyrifos leads to effects observed at both cellular and population level 2021-08-26T14:06:44Z 2021-08-26T14:06:44Z 2015-06-09 Artículo Asselborn, Viviana Magdalena, et al., 2015. Effects of chlorpyrifos on the growth and ultrastructure of green algae, ankistrodesmus gracilis. Ecotoxicology and Environmental Safety. Manchester: Elsevier, vol. 120, p. 334-341. ISSN 1090-2414. 0147-6513 http://repositorio.unne.edu.ar/handle/123456789/28489 eng openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf application/pdf Elsevier Ecotoxicology and Environmental Safety, 2015, vol. 120, p. 334-341.
institution	Universidad Nacional del Nordeste
institution_str	I-48
repository_str	R-184
collection	RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language	Inglés
topic	Pesticide Microalgae Toxicity Ultrastructure
spellingShingle	Pesticide Microalgae Toxicity Ultrastructure Asselborn, Viviana Magdalena Fernández, Carolina Zalocar de Domitrovic, Yolanda Parodi, Elisa Rosalía Effects of chlorpyrifos on the growth and ultrastructure of green algae, ankistrodesmus gracilis
topic_facet	Pesticide Microalgae Toxicity Ultrastructure
description	The effect of the organophosphorus insecticide chlorpyrifos on the growth, biovolume, and ultrastructure of the green microalga Ankistrodesmus gracilis was evaluated. Concentrations of 9.37, 18.75, 37.5, 75 and 150 mg L 1 of chlorpyrifos were assayed along with a control culture. At the end of the bioassay the ultrastructure of algal cells from control culture and from cultures exposed to 37.5 and 150 mg L 1 was observed under transmission (TEM) and scanning electron microscopy (SEM). After 24 and 48 h, treatments with 75 and 150 mg L 1 inhibited the growth of A. gracilis; whereas after 72 and 96 h, all the treatments except at 9.37 mg L 1 significantly affected the algae growth. The effective concentration 50 (EC50) after 96 h was 22.44 mg L 1 of chlorpyrifos. After the exposure to the insecticide, an increase in the biovolume was observed, with a larger increase in cells exposed to 75 and 150 mg L 1 . Radical changes were observed in the ultrastructure of cells exposed to chlorpyrifos. The insecticide affected the cell shape and the distribution of the crests in the wall. At 37.5 mg L 1 electodense bodies were observed along with an increase in the size and number of starch granules. At 150 mg L 1 such bodies occupied almost the whole cytoplasm together with lipids and remains of thylakoids. Autospores formation occurred normally at 37.5 mg L 1 while at 150 mg L 1 karyokinesis occurred, but cell-separation-phase was inhibited. The present study demonstrates that the exposure of phytoplankton to the insecticide chlorpyrifos leads to effects observed at both cellular and population level
format	Artículo
author	Asselborn, Viviana Magdalena Fernández, Carolina Zalocar de Domitrovic, Yolanda Parodi, Elisa Rosalía
author_facet	Asselborn, Viviana Magdalena Fernández, Carolina Zalocar de Domitrovic, Yolanda Parodi, Elisa Rosalía
author_sort	Asselborn, Viviana Magdalena
title	Effects of chlorpyrifos on the growth and ultrastructure of green algae, ankistrodesmus gracilis
title_short	Effects of chlorpyrifos on the growth and ultrastructure of green algae, ankistrodesmus gracilis
title_full	Effects of chlorpyrifos on the growth and ultrastructure of green algae, ankistrodesmus gracilis
title_fullStr	Effects of chlorpyrifos on the growth and ultrastructure of green algae, ankistrodesmus gracilis
title_full_unstemmed	Effects of chlorpyrifos on the growth and ultrastructure of green algae, ankistrodesmus gracilis
title_sort	effects of chlorpyrifos on the growth and ultrastructure of green algae, ankistrodesmus gracilis
publisher	Elsevier
publishDate	2021
url	http://repositorio.unne.edu.ar/handle/123456789/28489
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Effects of chlorpyrifos on the growth and ultrastructure of green algae, ankistrodesmus gracilis

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