Tumor biology of non-metastatic stages of clear cell renal cell carcinoma; overexpression of stearoyl desaturase-1, EPO/EPO-R system and hypoxia-related proteins

Clear cell renal cell carcinoma (ccRCC) is the most common subtype of renal carcinoma. There is great interest to know the molecular basis of the tumor biology of ccRCC that might contribute to a better understanding of the aggressive biological behavior of this cancer and to identify early biomarke...

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Autores principales: Stoyanoff, Tania Romina, Rodríguez, Juan Pablo, Todaro, Juan Santiago, Espada, Joaquín Diego, Melana Colavita, Juan Pablo, Brandan, Nora Cristina, Torres, Adriana Mónica, Aguirre, María Victoria
Formato: Artículo
Lenguaje:Inglés
Publicado: Springer 2021
Materias:
Clear cell renal cell carcinoma (ccrcc)
Erythropoietin (epo)
Epo receptor (epo-r)
Apoptosis
Stearoyl desaturase-1 (scd-1)
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/28104
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-28104
record_format dspace
spelling I48-R184-123456789-281042024-12-16T11:34:52Z Tumor biology of non-metastatic stages of clear cell renal cell carcinoma; overexpression of stearoyl desaturase-1, EPO/EPO-R system and hypoxia-related proteins Stoyanoff, Tania Romina Rodríguez, Juan Pablo Todaro, Juan Santiago Espada, Joaquín Diego Melana Colavita, Juan Pablo Brandan, Nora Cristina Torres, Adriana Mónica Aguirre, María Victoria Clear cell renal cell carcinoma (ccrcc) Erythropoietin (epo) Epo receptor (epo-r) Apoptosis Stearoyl desaturase-1 (scd-1) Clear cell renal cell carcinoma (ccRCC) is the most common subtype of renal carcinoma. There is great interest to know the molecular basis of the tumor biology of ccRCC that might contribute to a better understanding of the aggressive biological behavior of this cancer and to identify early biomarkers of disease. This study describes the relationship among proliferation, survival, and apoptosis with the expression of key molecules related to tumoral hypoxia (hypoxiainducible factor (HIF)-1α, erythropoietin (EPO), vascular endothelial growth factor (VEGF)), their receptors (EPO-R, VEGFR-2), and stearoyl desaturase-1(SCD-1) in early stages of ccRCC. Tissue samples were obtained at the Urology Unit of the J.R. Vidal Hospital (Corrientes, Argentina), from patients who underwent radical nephrectomy for renal cancer between 2011 and 2014. Four experimental groups according to pathological stage and nuclear grade were organized: T1G1 (n = 6), T2G1 (n = 4), T1G2 (n = 7), and T2G2 (n = 7). The expression of HIF-1α, EPO, EPO-R, VEGF, VEGFR-2, BclxL, and SCD-1 were evaluated by immunohistochemistry, Western blotting, and/or RT-PCR. Apoptosis was assessed by the TUNEL in situ assay, and tumor proliferation was determined by Ki-67 immunohistochemistry. Data revealed that HIF-1α, EPO, EPO-R, VEGF, and VEGF-R2 were overexpressed in most samples. The T1G1 group showed the highest EPO levels, approximately 200 % compared with distal renal tissue. Bcl-xL overexpression was concomitant withtheenhancementofproliferativeindexes.SCD-1expression increased with the tumor size and nuclear grade. Moreover, the direct correlations observed between SCD-1/ HIF-1α and SCD-1/Ki-67 increments suggest a link among these molecules, which would determine tumor progression in early stages of ccRCC. Our results demonstrate the relationship among proliferation, survival, and apoptosis with the expression of key molecules related to tumoral hypoxia (HIF1α, EPO, VEGF), their receptors (EPO-R, VEGFR-2), and SCD-1 in early stages of ccRCC. 2021-06-09T21:50:09Z 2021-06-09T21:50:09Z 2016-07-28 Artículo Stoyanoff, Tania Romina, et. al., 2016. Tumor biology of non-metastatic stages of clear cell renal cell carcinoma; overexpression of stearoyl desaturase-1, EPO/EPO-R system and hypoxia-related proteins.Tumor Biology. Berlín: Springer, vol. 37, no. 10, p. 1-13. ISSN 1423-0380. 1423-0380 http://repositorio.unne.edu.ar/handle/123456789/28104 eng openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf application/pdf Springer Tumor Biology, 2016, vol. 37, no. 10, p. 1-13.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Clear cell renal cell carcinoma (ccrcc)
Erythropoietin (epo)
Epo receptor (epo-r)
Apoptosis
Stearoyl desaturase-1 (scd-1)
spellingShingle Clear cell renal cell carcinoma (ccrcc)
Erythropoietin (epo)
Epo receptor (epo-r)
Apoptosis
Stearoyl desaturase-1 (scd-1)
Stoyanoff, Tania Romina
Rodríguez, Juan Pablo
Todaro, Juan Santiago
Espada, Joaquín Diego
Melana Colavita, Juan Pablo
Brandan, Nora Cristina
Torres, Adriana Mónica
Aguirre, María Victoria
Tumor biology of non-metastatic stages of clear cell renal cell carcinoma; overexpression of stearoyl desaturase-1, EPO/EPO-R system and hypoxia-related proteins
topic_facet Clear cell renal cell carcinoma (ccrcc)
Erythropoietin (epo)
Epo receptor (epo-r)
Apoptosis
Stearoyl desaturase-1 (scd-1)
description Clear cell renal cell carcinoma (ccRCC) is the most common subtype of renal carcinoma. There is great interest to know the molecular basis of the tumor biology of ccRCC that might contribute to a better understanding of the aggressive biological behavior of this cancer and to identify early biomarkers of disease. This study describes the relationship among proliferation, survival, and apoptosis with the expression of key molecules related to tumoral hypoxia (hypoxiainducible factor (HIF)-1α, erythropoietin (EPO), vascular endothelial growth factor (VEGF)), their receptors (EPO-R, VEGFR-2), and stearoyl desaturase-1(SCD-1) in early stages of ccRCC. Tissue samples were obtained at the Urology Unit of the J.R. Vidal Hospital (Corrientes, Argentina), from patients who underwent radical nephrectomy for renal cancer between 2011 and 2014. Four experimental groups according to pathological stage and nuclear grade were organized: T1G1 (n = 6), T2G1 (n = 4), T1G2 (n = 7), and T2G2 (n = 7). The expression of HIF-1α, EPO, EPO-R, VEGF, VEGFR-2, BclxL, and SCD-1 were evaluated by immunohistochemistry, Western blotting, and/or RT-PCR. Apoptosis was assessed by the TUNEL in situ assay, and tumor proliferation was determined by Ki-67 immunohistochemistry. Data revealed that HIF-1α, EPO, EPO-R, VEGF, and VEGF-R2 were overexpressed in most samples. The T1G1 group showed the highest EPO levels, approximately 200 % compared with distal renal tissue. Bcl-xL overexpression was concomitant withtheenhancementofproliferativeindexes.SCD-1expression increased with the tumor size and nuclear grade. Moreover, the direct correlations observed between SCD-1/ HIF-1α and SCD-1/Ki-67 increments suggest a link among these molecules, which would determine tumor progression in early stages of ccRCC. Our results demonstrate the relationship among proliferation, survival, and apoptosis with the expression of key molecules related to tumoral hypoxia (HIF1α, EPO, VEGF), their receptors (EPO-R, VEGFR-2), and SCD-1 in early stages of ccRCC.
format Artículo
author Stoyanoff, Tania Romina
Rodríguez, Juan Pablo
Todaro, Juan Santiago
Espada, Joaquín Diego
Melana Colavita, Juan Pablo
Brandan, Nora Cristina
Torres, Adriana Mónica
Aguirre, María Victoria
author_facet Stoyanoff, Tania Romina
Rodríguez, Juan Pablo
Todaro, Juan Santiago
Espada, Joaquín Diego
Melana Colavita, Juan Pablo
Brandan, Nora Cristina
Torres, Adriana Mónica
Aguirre, María Victoria
author_sort Stoyanoff, Tania Romina
title Tumor biology of non-metastatic stages of clear cell renal cell carcinoma; overexpression of stearoyl desaturase-1, EPO/EPO-R system and hypoxia-related proteins
title_short Tumor biology of non-metastatic stages of clear cell renal cell carcinoma; overexpression of stearoyl desaturase-1, EPO/EPO-R system and hypoxia-related proteins
title_full Tumor biology of non-metastatic stages of clear cell renal cell carcinoma; overexpression of stearoyl desaturase-1, EPO/EPO-R system and hypoxia-related proteins
title_fullStr Tumor biology of non-metastatic stages of clear cell renal cell carcinoma; overexpression of stearoyl desaturase-1, EPO/EPO-R system and hypoxia-related proteins
title_full_unstemmed Tumor biology of non-metastatic stages of clear cell renal cell carcinoma; overexpression of stearoyl desaturase-1, EPO/EPO-R system and hypoxia-related proteins
title_sort tumor biology of non-metastatic stages of clear cell renal cell carcinoma; overexpression of stearoyl desaturase-1, epo/epo-r system and hypoxia-related proteins
publisher Springer
publishDate 2021
url http://repositorio.unne.edu.ar/handle/123456789/28104
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