Biotechnology applied to cassava propagation in Argentina

Cassava is a staple food to millions of people in tropical and subtropical countries. Although it is traditionally cultivated from stem cuttings, which is a simple and inexpensive technique, this method presents serious problems such as low multiplication rates, difficulties to conserve stems, and d...

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Autores principales: Cavallero, María Inés, Medina, Ricardo Daniel, Hoyos, Rosa Elena, Cenóz, Pedro Jorge, Mroginski, Luis Amado
Otros Autores: Pace, Colleen M.
Formato: parte de libro
Lenguaje:Inglés
Publicado: Nova Science Publishers 2021
Materias:
Acclimatization
Cassava
In vitro plant regeneration
Manihot escalenta
Tuberous roots
Yields
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/28004
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-28004
record_format dspace
spelling I48-R184-123456789-280042025-11-04T09:57:22Z Biotechnology applied to cassava propagation in Argentina Cavallero, María Inés Medina, Ricardo Daniel Hoyos, Rosa Elena Cenóz, Pedro Jorge Mroginski, Luis Amado Pace, Colleen M. Acclimatization Cassava In vitro plant regeneration Manihot escalenta Tuberous roots Yields Cassava is a staple food to millions of people in tropical and subtropical countries. Although it is traditionally cultivated from stem cuttings, which is a simple and inexpensive technique, this method presents serious problems such as low multiplication rates, difficulties to conserve stems, and dissemination of pests and diseases. Many of these problems would be solved through in vitro tissue culture. In this work, we evaluated the in vitro establishment and multiplication of 28 cassava clones of agronomic interest for the Northeastern Argentina, a boundary area for this crop. Since the transfer of in vitro plants to ex vitro conditions is a critical phase of micropropagation, we evaluated the effect of different acclimatization treatments on survival and growth parameters of plants (cv EC118) grown in a culture chamber. We also scored their field survival and performance by comparing them with plants obtained by the conventional planting technique. After disinfection, uninodal segment culture in Murashige and Skoog medium supplemented with 0.01 mg/L BAP + 0.01 mg/L NAA + 0.1 mg/L GA3 allowed the in vitro establishment of 100% of the clones and their subsequent multiplication. Cultures were maintained at 27°±2°C with a 14 h photoperiod. During establishment, sprouting occurred in 100% of the clones and rooting in 93% of them; the remaining clones formed roots during the multiplication phase. Thirty days after multiplication, the plants presented significant differences in plant height, average number of nodes per plant and number of roots per plant. During acclimatization, five treatments were evaluated: three substrates (perlite, T1; sand + vermicompost, T2; commercial substrate composed of peat and perlite, T3), and two hydroponic treatments (tapwater, T4; Arnon and Hoagland nutrient solution, T5). Although in chamber growth conditions the acclimatized plants showed statistical differences in several growth parameters depending on the treatments, no differences were observed in the survival percentage. Shoot and root fresh and dry weight and leaf area were highest in T5 and lowest in T2 and T4. Field survival differed significantly between treatments, discriminating a group with high survival rates (T5: 73.3%, T3: 86.7%, and control treatment: 100%) and another with low survival rates (T2: 33.3%; T1: 35% and T4: 36.7%). At harvest, there were no significant differences in the total fresh weight. However, the percentage of biomass partitioned to roots was significantly higher in T3 and T5, which resulted in a higher tuberous roots yield than that of the control treatment. 2021-05-31T17:29:54Z 2021-05-31T17:29:54Z 2012 parte de libro Cavallero, María Inés, et al., 2012. Biotechnology applied to cassava propagation in Argentina. En: Pace, Colleen M., ed. Cassava: farming, uses, and economic impact. New York: Nova Science Publishers, p. 55-77. ISBN 978-1-61209-655-1. 978-1-61209-655-1 http://repositorio.unne.edu.ar/handle/123456789/28004 eng openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 55-77 application/pdf Nova Science Publishers
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Acclimatization
Cassava
In vitro plant regeneration
Manihot escalenta
Tuberous roots
Yields
spellingShingle Acclimatization
Cassava
In vitro plant regeneration
Manihot escalenta
Tuberous roots
Yields
Cavallero, María Inés
Medina, Ricardo Daniel
Hoyos, Rosa Elena
Cenóz, Pedro Jorge
Mroginski, Luis Amado
Biotechnology applied to cassava propagation in Argentina
topic_facet Acclimatization
Cassava
In vitro plant regeneration
Manihot escalenta
Tuberous roots
Yields
description Cassava is a staple food to millions of people in tropical and subtropical countries. Although it is traditionally cultivated from stem cuttings, which is a simple and inexpensive technique, this method presents serious problems such as low multiplication rates, difficulties to conserve stems, and dissemination of pests and diseases. Many of these problems would be solved through in vitro tissue culture. In this work, we evaluated the in vitro establishment and multiplication of 28 cassava clones of agronomic interest for the Northeastern Argentina, a boundary area for this crop. Since the transfer of in vitro plants to ex vitro conditions is a critical phase of micropropagation, we evaluated the effect of different acclimatization treatments on survival and growth parameters of plants (cv EC118) grown in a culture chamber. We also scored their field survival and performance by comparing them with plants obtained by the conventional planting technique. After disinfection, uninodal segment culture in Murashige and Skoog medium supplemented with 0.01 mg/L BAP + 0.01 mg/L NAA + 0.1 mg/L GA3 allowed the in vitro establishment of 100% of the clones and their subsequent multiplication. Cultures were maintained at 27°±2°C with a 14 h photoperiod. During establishment, sprouting occurred in 100% of the clones and rooting in 93% of them; the remaining clones formed roots during the multiplication phase. Thirty days after multiplication, the plants presented significant differences in plant height, average number of nodes per plant and number of roots per plant. During acclimatization, five treatments were evaluated: three substrates (perlite, T1; sand + vermicompost, T2; commercial substrate composed of peat and perlite, T3), and two hydroponic treatments (tapwater, T4; Arnon and Hoagland nutrient solution, T5). Although in chamber growth conditions the acclimatized plants showed statistical differences in several growth parameters depending on the treatments, no differences were observed in the survival percentage. Shoot and root fresh and dry weight and leaf area were highest in T5 and lowest in T2 and T4. Field survival differed significantly between treatments, discriminating a group with high survival rates (T5: 73.3%, T3: 86.7%, and control treatment: 100%) and another with low survival rates (T2: 33.3%; T1: 35% and T4: 36.7%). At harvest, there were no significant differences in the total fresh weight. However, the percentage of biomass partitioned to roots was significantly higher in T3 and T5, which resulted in a higher tuberous roots yield than that of the control treatment.
author2 Pace, Colleen M.
author_facet Pace, Colleen M.
Cavallero, María Inés
Medina, Ricardo Daniel
Hoyos, Rosa Elena
Cenóz, Pedro Jorge
Mroginski, Luis Amado
format parte de libro
author Cavallero, María Inés
Medina, Ricardo Daniel
Hoyos, Rosa Elena
Cenóz, Pedro Jorge
Mroginski, Luis Amado
author_sort Cavallero, María Inés
title Biotechnology applied to cassava propagation in Argentina
title_short Biotechnology applied to cassava propagation in Argentina
title_full Biotechnology applied to cassava propagation in Argentina
title_fullStr Biotechnology applied to cassava propagation in Argentina
title_full_unstemmed Biotechnology applied to cassava propagation in Argentina
title_sort biotechnology applied to cassava propagation in argentina
publisher Nova Science Publishers
publishDate 2021
url http://repositorio.unne.edu.ar/handle/123456789/28004
work_keys_str_mv AT cavalleromariaines biotechnologyappliedtocassavapropagationinargentina
AT medinaricardodaniel biotechnologyappliedtocassavapropagationinargentina
AT hoyosrosaelena biotechnologyappliedtocassavapropagationinargentina
AT cenozpedrojorge biotechnologyappliedtocassavapropagationinargentina
AT mroginskiluisamado biotechnologyappliedtocassavapropagationinargentina
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