Chemometric optimization and validation of a novel dispersive liquid liquid microextraction HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples

A dispersive liquid–liquid micro extraction (DLLME) liquid chromatographic method that allows extraction and separation of gliclazide, glibenclamide and glimepiride from serum was developed and optimized with the use of experimental design. The analyzed factors in optimization were type of extractin...

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Autores principales: Monzón, Celina María, Teglia, Carla M., Delfino, Mario Raúl, Goicoechea, Héctor C.
Formato: Artículo
Lenguaje:Inglés
Publicado: Elsevier 2021
Materias:
DLLME
Glibenclamide
Gliclazide
Glimepiride
Chemometrics
HPLC
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/27940
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-27940
record_format dspace
spelling I48-R184-123456789-279402025-03-06T11:03:38Z Chemometric optimization and validation of a novel dispersive liquid liquid microextraction HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples Monzón, Celina María Teglia, Carla M. Delfino, Mario Raúl Goicoechea, Héctor C. DLLME Glibenclamide Gliclazide Glimepiride Chemometrics HPLC A dispersive liquid–liquid micro extraction (DLLME) liquid chromatographic method that allows extraction and separation of gliclazide, glibenclamide and glimepiride from serum was developed and optimized with the use of experimental design. The analyzed factors in optimization were type of extracting solvent, extracting solvent volume, dispersing solvent volume, pH and protein precipitation. The selected conditions for DLLME were dichloromethane 100 μL (extracting solvent), acetonitrile 1000 μL (dispersing solvent) and no protein precipitation. This procedure is simple, requires no sophisticated procedures and produces excellent analyte recoveries. Quantitation of glibenclamide, gliclazide and glimepiride in serum samples was carried out by HPLC. This analytical method has been developed and validated, allowing quantitation of the target analytes in presence of atenolol, enalapril and amlodipine besides other serum sample interferents. The chromatographic method is linear, accurate, precise and specific and has the ability to separate the antihyperglycemic drugs from antihypertensive drugs, which are usually found in serum of diabetic patients (LOQs ca. 0.12 μg L−1 for the three analytes). 2021-05-17T15:20:28Z 2021-05-17T15:20:28Z 2016 Artículo Monzón, Celina M., et al., 2016. Chemometric optimization and validation of a novel dispersive liquid liquid microextraction HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples. Microchemical Journal. Ámsterdam: Elsevier, vol. 127, p. 113-119. ISSN 0026-265X. 0026-265X http://repositorio.unne.edu.ar/handle/123456789/27940 eng http://dx.doi.org/10.1016/j.microc.2016.02.011 openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf application/pdf Elsevier Microchemical Journal, 2016, vol. 127, p. 113-119.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic DLLME
Glibenclamide
Gliclazide
Glimepiride
Chemometrics
HPLC
spellingShingle DLLME
Glibenclamide
Gliclazide
Glimepiride
Chemometrics
HPLC
Monzón, Celina María
Teglia, Carla M.
Delfino, Mario Raúl
Goicoechea, Héctor C.
Chemometric optimization and validation of a novel dispersive liquid liquid microextraction HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples
topic_facet DLLME
Glibenclamide
Gliclazide
Glimepiride
Chemometrics
HPLC
description A dispersive liquid–liquid micro extraction (DLLME) liquid chromatographic method that allows extraction and separation of gliclazide, glibenclamide and glimepiride from serum was developed and optimized with the use of experimental design. The analyzed factors in optimization were type of extracting solvent, extracting solvent volume, dispersing solvent volume, pH and protein precipitation. The selected conditions for DLLME were dichloromethane 100 μL (extracting solvent), acetonitrile 1000 μL (dispersing solvent) and no protein precipitation. This procedure is simple, requires no sophisticated procedures and produces excellent analyte recoveries. Quantitation of glibenclamide, gliclazide and glimepiride in serum samples was carried out by HPLC. This analytical method has been developed and validated, allowing quantitation of the target analytes in presence of atenolol, enalapril and amlodipine besides other serum sample interferents. The chromatographic method is linear, accurate, precise and specific and has the ability to separate the antihyperglycemic drugs from antihypertensive drugs, which are usually found in serum of diabetic patients (LOQs ca. 0.12 μg L−1 for the three analytes).
format Artículo
author Monzón, Celina María
Teglia, Carla M.
Delfino, Mario Raúl
Goicoechea, Héctor C.
author_facet Monzón, Celina María
Teglia, Carla M.
Delfino, Mario Raúl
Goicoechea, Héctor C.
author_sort Monzón, Celina María
title Chemometric optimization and validation of a novel dispersive liquid liquid microextraction HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples
title_short Chemometric optimization and validation of a novel dispersive liquid liquid microextraction HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples
title_full Chemometric optimization and validation of a novel dispersive liquid liquid microextraction HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples
title_fullStr Chemometric optimization and validation of a novel dispersive liquid liquid microextraction HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples
title_full_unstemmed Chemometric optimization and validation of a novel dispersive liquid liquid microextraction HPLC method for gliclazide, glibenclamide and glimepiride quantitation in serum samples
title_sort chemometric optimization and validation of a novel dispersive liquid liquid microextraction hplc method for gliclazide, glibenclamide and glimepiride quantitation in serum samples
publisher Elsevier
publishDate 2021
url http://repositorio.unne.edu.ar/handle/123456789/27940
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