Anatomical and histological features of Ilex paraguariensis leaves under different in vitro shoot culture systems

Ilex paraguariensis (Aquifoliaceae) is an evergreen tree traditionally used to prepare a caffeine-rich infusion that has several medicinal properties. The in vitro propagation of this species has been studied as an alternative to conventional methods such as cuttings and seedlings. The in vitro cult...

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Autores principales: Luna, Claudia Verónica, González, Ana María, Mroginski, Luis Amado, Sansberro, Pedro Alfonso
Formato: Artículo
Lenguaje:Inglés
Publicado: Springer 2021
Materias:
Micropropagation
Leaf histology
Temporary immersion
Ilex paraguariensis
Leaf anatomy
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/27876
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-27876
record_format dspace
spelling I48-R184-123456789-278762024-09-27T12:49:48Z Anatomical and histological features of Ilex paraguariensis leaves under different in vitro shoot culture systems Luna, Claudia Verónica González, Ana María Mroginski, Luis Amado Sansberro, Pedro Alfonso Micropropagation Leaf histology Temporary immersion Ilex paraguariensis Leaf anatomy Ilex paraguariensis (Aquifoliaceae) is an evergreen tree traditionally used to prepare a caffeine-rich infusion that has several medicinal properties. The in vitro propagation of this species has been studied as an alternative to conventional methods such as cuttings and seedlings. The in vitro culture environment consists of closed flasks with high relative humidity, reduced gas exchange and low luminosity conditions, which may induce physiological, anatomical and histological disruption in the cultivated plants. The occurrence of anatomical and histological alterations to the leaves of I. paraguariensis was examined in plants cultivated under greenhouse and different in vitro conditions. The variations in the microclimate inside the culture vessels generated by the different treatments tested affected the anatomy and histology of I. paraguariensis leaves by causing alterations in their architecture, thickness, vascularization and stomatal differentiation. A temporary immersion system was the best treatment for conserving the anatomical and histological characteristics of the leaves. This treatment led to the lowest stomatal index and an extensive system of intercellular spaces that were similar to the characteristics developed under external environment conditions. 2021-05-04T15:52:56Z 2021-05-04T15:52:56Z 2017 Artículo Luna, C.V., et al., 2017. Anatomical and histological features of Ilex paraguariensis leaves under different in vitro shoot culture systems. Plant Cell Tiss Organ Cult, Berlín: Springer, no. 129, p. 457-467. ISSN 1573-5044. 0167-6857 http://repositorio.unne.edu.ar/handle/123456789/27876 eng https://link.springer.com/article/10.1007/s11240-017-1191-x openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf application/pdf Springer Plant Cell Tiss Organ Cult, 2017, no. 129, p. 457–467.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Micropropagation
Leaf histology
Temporary immersion
Ilex paraguariensis
Leaf anatomy
spellingShingle Micropropagation
Leaf histology
Temporary immersion
Ilex paraguariensis
Leaf anatomy
Luna, Claudia Verónica
González, Ana María
Mroginski, Luis Amado
Sansberro, Pedro Alfonso
Anatomical and histological features of Ilex paraguariensis leaves under different in vitro shoot culture systems
topic_facet Micropropagation
Leaf histology
Temporary immersion
Ilex paraguariensis
Leaf anatomy
description Ilex paraguariensis (Aquifoliaceae) is an evergreen tree traditionally used to prepare a caffeine-rich infusion that has several medicinal properties. The in vitro propagation of this species has been studied as an alternative to conventional methods such as cuttings and seedlings. The in vitro culture environment consists of closed flasks with high relative humidity, reduced gas exchange and low luminosity conditions, which may induce physiological, anatomical and histological disruption in the cultivated plants. The occurrence of anatomical and histological alterations to the leaves of I. paraguariensis was examined in plants cultivated under greenhouse and different in vitro conditions. The variations in the microclimate inside the culture vessels generated by the different treatments tested affected the anatomy and histology of I. paraguariensis leaves by causing alterations in their architecture, thickness, vascularization and stomatal differentiation. A temporary immersion system was the best treatment for conserving the anatomical and histological characteristics of the leaves. This treatment led to the lowest stomatal index and an extensive system of intercellular spaces that were similar to the characteristics developed under external environment conditions.
format Artículo
author Luna, Claudia Verónica
González, Ana María
Mroginski, Luis Amado
Sansberro, Pedro Alfonso
author_facet Luna, Claudia Verónica
González, Ana María
Mroginski, Luis Amado
Sansberro, Pedro Alfonso
author_sort Luna, Claudia Verónica
title Anatomical and histological features of Ilex paraguariensis leaves under different in vitro shoot culture systems
title_short Anatomical and histological features of Ilex paraguariensis leaves under different in vitro shoot culture systems
title_full Anatomical and histological features of Ilex paraguariensis leaves under different in vitro shoot culture systems
title_fullStr Anatomical and histological features of Ilex paraguariensis leaves under different in vitro shoot culture systems
title_full_unstemmed Anatomical and histological features of Ilex paraguariensis leaves under different in vitro shoot culture systems
title_sort anatomical and histological features of ilex paraguariensis leaves under different in vitro shoot culture systems
publisher Springer
publishDate 2021
url http://repositorio.unne.edu.ar/handle/123456789/27876
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AT gonzalezanamaria anatomicalandhistologicalfeaturesofilexparaguariensisleavesunderdifferentinvitroshootculturesystems
AT mroginskiluisamado anatomicalandhistologicalfeaturesofilexparaguariensisleavesunderdifferentinvitroshootculturesystems
AT sansberropedroalfonso anatomicalandhistologicalfeaturesofilexparaguariensisleavesunderdifferentinvitroshootculturesystems
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