Micropropagation of handroanthus heptaphyllus (Vell.) mattos from seedling explants

Handroanthus heptaphyllus (Vell.) Mattos (Bignoniaceae), a tropical forest tree, is a source of wood suitable for the manufacture of fine furniture and chemical compounds with medicinal and insecticidalproperties. Poor seed viability of this species limits the conventional propagation practice. In v...

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Autores principales: Duarte, Evelyn Raquel, Sansberro, Pedro Alfonso, Luna, Claudia Verónica
Formato: Artículo
Lenguaje:Inglés
Publicado: Academic Journals 2021
Materias:
Axillary shoots
Adventitious shoots
Micropropagation
Seed
Uncoated seeds
Cotyledon
Stem nodes
Black lapacho
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/27788
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-27788
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spelling I48-R184-123456789-277882024-10-09T14:48:05Z Micropropagation of handroanthus heptaphyllus (Vell.) mattos from seedling explants Duarte, Evelyn Raquel Sansberro, Pedro Alfonso Luna, Claudia Verónica Axillary shoots Adventitious shoots Micropropagation Seed Uncoated seeds Cotyledon Stem nodes Black lapacho Handroanthus heptaphyllus (Vell.) Mattos (Bignoniaceae), a tropical forest tree, is a source of wood suitable for the manufacture of fine furniture and chemical compounds with medicinal and insecticidalproperties. Poor seed viability of this species limits the conventional propagation practice. In vitro germination of seeds was achieved in semi-solid Murashige and Skoog?s (MS) medium. A disinfectionprotocol was optimized for germination and growth of seedlings in vitro. Proliferation of shoots from cotyledonary and stem nodal segments was achieved in semi-solid MS medium supplemented with 22.2 μM 6-benzyladenine. Temporary immersion culture promoted elongation and rooting of shoots andreduced the shoot tip necrosis compared with culture on semisolid medium (6.7±2.7 and 41.7±7.0%,respectively; P = 0.001). Plantlets derived from temporary immersion showed better performance during the acclimatization phase. This is the first report on micropropagation of H. heptaphyllus 2021-04-14T19:16:54Z 2021-04-14T19:16:54Z 2016-06-22 Artículo Duarte, Evelyn Raquel, Sansberro, Pedro Alfonso y Luna, Claudia Verónica, 2016. Micropropagation of handroanthus heptaphyllus (Vell.) mattos from seedling explants. African Journal of Biotechnology. Nairobi: Academic Journals, vol. 15, no. 25, p. 1292-1298. ISSN 1684-5315. 1684-5315 http://repositorio.unne.edu.ar/handle/123456789/27788 eng https://academicjournals.org/journal/AJB/article-abstract/059F2CD59041 openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf application/pdf Academic Journals African Journal of Biotechnology, 2016, vol. 15, no. 25, p. 1292-1298.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Axillary shoots
Adventitious shoots
Micropropagation
Seed
Uncoated seeds
Cotyledon
Stem nodes
Black lapacho
spellingShingle Axillary shoots
Adventitious shoots
Micropropagation
Seed
Uncoated seeds
Cotyledon
Stem nodes
Black lapacho
Duarte, Evelyn Raquel
Sansberro, Pedro Alfonso
Luna, Claudia Verónica
Micropropagation of handroanthus heptaphyllus (Vell.) mattos from seedling explants
topic_facet Axillary shoots
Adventitious shoots
Micropropagation
Seed
Uncoated seeds
Cotyledon
Stem nodes
Black lapacho
description Handroanthus heptaphyllus (Vell.) Mattos (Bignoniaceae), a tropical forest tree, is a source of wood suitable for the manufacture of fine furniture and chemical compounds with medicinal and insecticidalproperties. Poor seed viability of this species limits the conventional propagation practice. In vitro germination of seeds was achieved in semi-solid Murashige and Skoog?s (MS) medium. A disinfectionprotocol was optimized for germination and growth of seedlings in vitro. Proliferation of shoots from cotyledonary and stem nodal segments was achieved in semi-solid MS medium supplemented with 22.2 μM 6-benzyladenine. Temporary immersion culture promoted elongation and rooting of shoots andreduced the shoot tip necrosis compared with culture on semisolid medium (6.7±2.7 and 41.7±7.0%,respectively; P = 0.001). Plantlets derived from temporary immersion showed better performance during the acclimatization phase. This is the first report on micropropagation of H. heptaphyllus
format Artículo
author Duarte, Evelyn Raquel
Sansberro, Pedro Alfonso
Luna, Claudia Verónica
author_facet Duarte, Evelyn Raquel
Sansberro, Pedro Alfonso
Luna, Claudia Verónica
author_sort Duarte, Evelyn Raquel
title Micropropagation of handroanthus heptaphyllus (Vell.) mattos from seedling explants
title_short Micropropagation of handroanthus heptaphyllus (Vell.) mattos from seedling explants
title_full Micropropagation of handroanthus heptaphyllus (Vell.) mattos from seedling explants
title_fullStr Micropropagation of handroanthus heptaphyllus (Vell.) mattos from seedling explants
title_full_unstemmed Micropropagation of handroanthus heptaphyllus (Vell.) mattos from seedling explants
title_sort micropropagation of handroanthus heptaphyllus (vell.) mattos from seedling explants
publisher Academic Journals
publishDate 2021
url http://repositorio.unne.edu.ar/handle/123456789/27788
work_keys_str_mv AT duarteevelynraquel micropropagationofhandroanthusheptaphyllusvellmattosfromseedlingexplants
AT sansberropedroalfonso micropropagationofhandroanthusheptaphyllusvellmattosfromseedlingexplants
AT lunaclaudiaveronica micropropagationofhandroanthusheptaphyllusvellmattosfromseedlingexplants
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