Long-term preservation of Lotus tenuis adventitious buds

Encapsulation-dehydration, encapsulation-vitrification, and vitrification were tested for cryopreservation of Lotus tenuis (Fagaceae) adventitious buds clusters (ABCs) obtained by a direct regeneration system from leaves cultures. Among them, the PVS3-based vitrification procedure was found to be...

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Detalles Bibliográficos
Autores principales: Espasandin, Fabiana Daniela, Brugnoli, Elsa Andrea, Ayala, Paula Gabriela, Ayala, Lilian P., Ruiz, Oscar Adolfo, Sansberro, Pedro Alfonso
Formato: Artículo
Lenguaje:Inglés
Publicado: Springer Nature 2021
Materias:
Cryopreservation
PVS3
Vitrification
ISSR markers
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/27779
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-27779
record_format dspace
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Cryopreservation
PVS3
Vitrification
ISSR markers
spellingShingle Cryopreservation
PVS3
Vitrification
ISSR markers
Espasandin, Fabiana Daniela
Brugnoli, Elsa Andrea
Ayala, Paula Gabriela
Ayala, Lilian P.
Ruiz, Oscar Adolfo
Sansberro, Pedro Alfonso
Long-term preservation of Lotus tenuis adventitious buds
topic_facet Cryopreservation
PVS3
Vitrification
ISSR markers
description Encapsulation-dehydration, encapsulation-vitrification, and vitrification were tested for cryopreservation of Lotus tenuis (Fagaceae) adventitious buds clusters (ABCs) obtained by a direct regeneration system from leaves cultures. Among them, the PVS3-based vitrification procedure was found to be useful for survival and regrowth of the preserved explants. For vitrification, the ABCs were dehydrated in a solution containing 2 M glycerol + 0.4 M sucrose for 25 min at room temperature, submerged in PVS3 solution for 1 h at 0 °C, then immersed in liquid nitrogen for 48 h and rapidly rewarmed. Afterword, the explants were unloaded in MS liquid medium with 1.2 M sucrose for 30 min. The washed tissues were dried superficially on filter paper and cultured in semisolid hormone-free MS medium containing 0.1 M sucrose. All cultures were maintained at 25 °C in the dark for 10 days and transferred to the light conditions. With this procedure, 79 ± 5.3% survival and more than 80% of the plantlets displaying a phenotype similar to the non-treated control after acclimatization. The data settled from ISSR showed no genetic dissimilarities between in vitro regenerants derived from cryopreserved tissues and the non-treated plants. Thus, our results indicate that the use of vitrification-based PVS3 solution offers a simple, accurate, and appropriate procedure for the cryopreservation of L. tenuis adventitious buds.
format Artículo
author Espasandin, Fabiana Daniela
Brugnoli, Elsa Andrea
Ayala, Paula Gabriela
Ayala, Lilian P.
Ruiz, Oscar Adolfo
Sansberro, Pedro Alfonso
author_facet Espasandin, Fabiana Daniela
Brugnoli, Elsa Andrea
Ayala, Paula Gabriela
Ayala, Lilian P.
Ruiz, Oscar Adolfo
Sansberro, Pedro Alfonso
author_sort Espasandin, Fabiana Daniela
title Long-term preservation of Lotus tenuis adventitious buds
title_short Long-term preservation of Lotus tenuis adventitious buds
title_full Long-term preservation of Lotus tenuis adventitious buds
title_fullStr Long-term preservation of Lotus tenuis adventitious buds
title_full_unstemmed Long-term preservation of Lotus tenuis adventitious buds
title_sort long-term preservation of lotus tenuis adventitious buds
publisher Springer Nature
publishDate 2021
url http://repositorio.unne.edu.ar/handle/123456789/27779
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