Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2-fixing community depending on depth and agricultural use of soil

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In this survey, a total of 80 787 reads and 28 171 unique NifH protein sequences were retrieved from soil RNA. This dataset extends our knowledge about the structure and diversity of the functional diazotrophic communities in agricultural soils from Argentinean Pampas. Operational taxonomic unit (...

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Autores principales: Calderoli, Priscila A., Collavino, Mónica Mariana, Behrends Kraemer, Filipe, Morrás, Héctor J. M., Aguilar, Mario O.
Formato: Artículo
Lenguaje:Inglés
Publicado: Blackwell Publishing 2021
Materias:
Cyanobacteria and Geobacter
NifH transcripts
Soil diazotrophic community
Soil management
Practices
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/27726
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-27726
record_format dspace
spelling I48-R184-123456789-277262024-09-27T12:45:34Z Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2-fixing community depending on depth and agricultural use of soil Calderoli, Priscila A. Collavino, Mónica Mariana Behrends Kraemer, Filipe Morrás, Héctor J. M. Aguilar, Mario O. Cyanobacteria and Geobacter NifH transcripts Soil diazotrophic community Soil management Practices In this survey, a total of 80 787 reads and 28 171 unique NifH protein sequences were retrieved from soil RNA. This dataset extends our knowledge about the structure and diversity of the functional diazotrophic communities in agricultural soils from Argentinean Pampas. Operational taxonomic unit (OTU)-based analyses showed that nifH phylotypes related to Geobacter and Anaeromyxobacter (44.8%), Rhizobiales (29%), Cyanobacteria (16.7%), and Verrucomicrobiales (8%) are key microbial components of N2 fixation in soils associated with no-till management and soil depth. In addition, quantification of nifH gene copies related to Geobacter and Cyanobacteria revealed that these groups are abundant in soils under maize–soybean rotation and soybean monoculture, respectively. The correlation of physicochemical soil parameters with the diazotrophic diversity and composition showed that soil stability and organic carbón might contribute to the functional signatures of particular nifH phylotypes in fieldsunder no-till management. Because crop production relies on soil-borne microorganism’s activities, such as free N2 fixation, the information provided by our study on the diazotrophic population dynamics, associated with the edaphic properties and land-use practices, represents a major contribution to gain insight into soil biology, in which functionally active components are identified. 2021-03-26T16:00:28Z 2021-03-26T16:00:28Z 2017-05 Artículo Calderoli, Priscila A., et al., 2017. Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2-fixing community depending on depth and agricultural use of soil. MicrobiologyOpen. Oxford: Blackwell Publishing, vol. 6, no. 5, p. 1-15. ISSN 2045-8827. 2045-8827 http://repositorio.unne.edu.ar/handle/123456789/27726 eng https://doi.org/10.1002/mbo3.502 https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.502 openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf application/pdf Blackwell Publishing MicrobiologyOpen, 2017, vol. 6, no. 5, p. 1-15.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Cyanobacteria and Geobacter
NifH transcripts
Soil diazotrophic community
Soil management
Practices
spellingShingle Cyanobacteria and Geobacter
NifH transcripts
Soil diazotrophic community
Soil management
Practices
Calderoli, Priscila A.
Collavino, Mónica Mariana
Behrends Kraemer, Filipe
Morrás, Héctor J. M.
Aguilar, Mario O.
Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2-fixing community depending on depth and agricultural use of soil
topic_facet Cyanobacteria and Geobacter
NifH transcripts
Soil diazotrophic community
Soil management
Practices
description In this survey, a total of 80 787 reads and 28 171 unique NifH protein sequences were retrieved from soil RNA. This dataset extends our knowledge about the structure and diversity of the functional diazotrophic communities in agricultural soils from Argentinean Pampas. Operational taxonomic unit (OTU)-based analyses showed that nifH phylotypes related to Geobacter and Anaeromyxobacter (44.8%), Rhizobiales (29%), Cyanobacteria (16.7%), and Verrucomicrobiales (8%) are key microbial components of N2 fixation in soils associated with no-till management and soil depth. In addition, quantification of nifH gene copies related to Geobacter and Cyanobacteria revealed that these groups are abundant in soils under maize–soybean rotation and soybean monoculture, respectively. The correlation of physicochemical soil parameters with the diazotrophic diversity and composition showed that soil stability and organic carbón might contribute to the functional signatures of particular nifH phylotypes in fieldsunder no-till management. Because crop production relies on soil-borne microorganism’s activities, such as free N2 fixation, the information provided by our study on the diazotrophic population dynamics, associated with the edaphic properties and land-use practices, represents a major contribution to gain insight into soil biology, in which functionally active components are identified.
format Artículo
author Calderoli, Priscila A.
Collavino, Mónica Mariana
Behrends Kraemer, Filipe
Morrás, Héctor J. M.
Aguilar, Mario O.
author_facet Calderoli, Priscila A.
Collavino, Mónica Mariana
Behrends Kraemer, Filipe
Morrás, Héctor J. M.
Aguilar, Mario O.
author_sort Calderoli, Priscila A.
title Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2-fixing community depending on depth and agricultural use of soil
title_short Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2-fixing community depending on depth and agricultural use of soil
title_full Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2-fixing community depending on depth and agricultural use of soil
title_fullStr Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2-fixing community depending on depth and agricultural use of soil
title_full_unstemmed Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2-fixing community depending on depth and agricultural use of soil
title_sort analysis of nifh-rna reveals phylotypes related to geobacter and cyanobacteria as important functional components of the n2-fixing community depending on depth and agricultural use of soil
publisher Blackwell Publishing
publishDate 2021
url http://repositorio.unne.edu.ar/handle/123456789/27726
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