In vitro antibacterial effect of argentine propolis extracts on streptococcus mutans and actinomyces viscosus

Aim: In the present study, the antibacterial activity of the Ethanol Extract of Propolis (EEP), collected from various regions (Mendoza, Santiago del Estero, and Corrientes) in Argentina, against Streptococcus mutans ATCC® 35668™ and Actinomyces viscosus ATCC® 15987™ (MicroBioLogics Inc., USA) was...

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Autores principales: Rosende, Roque Oscar, Lozina, Laura Analía, Juárez, Rolando Pablo Alejandro
Formato: Artículo
Lenguaje:Portugués
Publicado: Universidade Federal de Minas Gerais 2020
Materias:
Propolis
Streptococcus mutans
Actinomyces viscosus
Microbial sensitivity tests
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/1654
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-1654
record_format dspace
spelling I48-R184-123456789-16542024-12-16T11:49:40Z In vitro antibacterial effect of argentine propolis extracts on streptococcus mutans and actinomyces viscosus Rosende, Roque Oscar Lozina, Laura Analía Juárez, Rolando Pablo Alejandro Propolis Streptococcus mutans Actinomyces viscosus Microbial sensitivity tests Aim: In the present study, the antibacterial activity of the Ethanol Extract of Propolis (EEP), collected from various regions (Mendoza, Santiago del Estero, and Corrientes) in Argentina, against Streptococcus mutans ATCC® 35668™ and Actinomyces viscosus ATCC® 15987™ (MicroBioLogics Inc., USA) was investigated. Methods: Identification of geographic and botanical origin was based on a reconnaissance survey. Phytochemical screening of propolis was carried out on ethanolic extracts using standard methods to identify the constituents (aluminum chloride colorimetric method, Folin-Ciocalteu colorimetric method, thin layer chromatography). The agar diffusion method (discs and wells) and serial dilution method (plates and tubes) were used to evaluate the antibacterial activity of EEP. Results: EEP exerted various degrees of antibacterial activity against S. mutans and A. viscosus, depending on the geographic area of collection. Phytochemical screening showed that the bioactive compounds correspond to phenolic compounds and flavones. EEP from Tunuyán (Mendoza), where the most abundant vegetation belongs to Populus sp., showed the highest content of phenolic compounds (220.92±2.01 mg/g) and flavonoids (30.39±0.25 mg/g). This sample showed the most profound antibacterial activity among the EEP tested. By the agar-well diffusion method, we found a high susceptibility with an inhibitory halo of 11.25 ± 4.68 mm and 10.90 ± 4.21 mm against S. mutans and A. viscosus, respectively. It also presented low Minimum Inhibitory Concentration and Minimum Bactericidal Concentration values against S. mutans (MIC 0.05 mg/mL - MBC 0.46 mg/mL) followed by A. viscosus (MIC 0.11 mg/mL - MBC 0.93 mg/mL). Conclusions: The combined results from all methods indicated that S. mutans is more susceptible to the effect of the Tunuyán EEP than A. viscosus. Fil: Rosende, Roque Oscar. Universidad Nacional del Nordeste. Facultad de Odontología; Argentina. Fil: Lozina, Laura Analía. Universidad Nacional del Nordeste. Facultad de Odontología; Argentina. Fil: Juárez, Rolando Pablo Alejandro. Universidad Nacional del Nordeste. Facultad de Odontología; Argentina. Fil: Juárez, Rolando Pablo Alejandro. Jefe de la División de Docencia e Investigación del Hospital Central de Odontología. Ministerio de Salud Pública de la Provincia del Chaco; Argentina. 2020-06-02T22:34:13Z 2020-06-02T22:34:13Z 2017 Artículo Rosende, Roque Oscar, Lozina, Laura Analía y JJuárez, Rolando Pablo Alejandro, 2017. In vitro antibacterial effect of Argentine propolis extracts on streptococcus mutans and actinomyces viscosus. Arq Odontol. Belo Horizonte: Universidade Federal de Minas Gerais, vol. 53, no. 19, p. 1-8. ISSN 2178-1990. 2178-1990 http://repositorio.unne.edu.ar/handle/123456789/1654 por openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 1-8 application/pdf Universidade Federal de Minas Gerais Arq Odontol, 2017, vol. 53, no. 19, p. 1-8.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Portugués
topic Propolis
Streptococcus mutans
Actinomyces viscosus
Microbial sensitivity tests
spellingShingle Propolis
Streptococcus mutans
Actinomyces viscosus
Microbial sensitivity tests
Rosende, Roque Oscar
Lozina, Laura Analía
Juárez, Rolando Pablo Alejandro
In vitro antibacterial effect of argentine propolis extracts on streptococcus mutans and actinomyces viscosus
topic_facet Propolis
Streptococcus mutans
Actinomyces viscosus
Microbial sensitivity tests
description Aim: In the present study, the antibacterial activity of the Ethanol Extract of Propolis (EEP), collected from various regions (Mendoza, Santiago del Estero, and Corrientes) in Argentina, against Streptococcus mutans ATCC® 35668™ and Actinomyces viscosus ATCC® 15987™ (MicroBioLogics Inc., USA) was investigated. Methods: Identification of geographic and botanical origin was based on a reconnaissance survey. Phytochemical screening of propolis was carried out on ethanolic extracts using standard methods to identify the constituents (aluminum chloride colorimetric method, Folin-Ciocalteu colorimetric method, thin layer chromatography). The agar diffusion method (discs and wells) and serial dilution method (plates and tubes) were used to evaluate the antibacterial activity of EEP. Results: EEP exerted various degrees of antibacterial activity against S. mutans and A. viscosus, depending on the geographic area of collection. Phytochemical screening showed that the bioactive compounds correspond to phenolic compounds and flavones. EEP from Tunuyán (Mendoza), where the most abundant vegetation belongs to Populus sp., showed the highest content of phenolic compounds (220.92±2.01 mg/g) and flavonoids (30.39±0.25 mg/g). This sample showed the most profound antibacterial activity among the EEP tested. By the agar-well diffusion method, we found a high susceptibility with an inhibitory halo of 11.25 ± 4.68 mm and 10.90 ± 4.21 mm against S. mutans and A. viscosus, respectively. It also presented low Minimum Inhibitory Concentration and Minimum Bactericidal Concentration values against S. mutans (MIC 0.05 mg/mL - MBC 0.46 mg/mL) followed by A. viscosus (MIC 0.11 mg/mL - MBC 0.93 mg/mL). Conclusions: The combined results from all methods indicated that S. mutans is more susceptible to the effect of the Tunuyán EEP than A. viscosus.
format Artículo
author Rosende, Roque Oscar
Lozina, Laura Analía
Juárez, Rolando Pablo Alejandro
author_facet Rosende, Roque Oscar
Lozina, Laura Analía
Juárez, Rolando Pablo Alejandro
author_sort Rosende, Roque Oscar
title In vitro antibacterial effect of argentine propolis extracts on streptococcus mutans and actinomyces viscosus
title_short In vitro antibacterial effect of argentine propolis extracts on streptococcus mutans and actinomyces viscosus
title_full In vitro antibacterial effect of argentine propolis extracts on streptococcus mutans and actinomyces viscosus
title_fullStr In vitro antibacterial effect of argentine propolis extracts on streptococcus mutans and actinomyces viscosus
title_full_unstemmed In vitro antibacterial effect of argentine propolis extracts on streptococcus mutans and actinomyces viscosus
title_sort in vitro antibacterial effect of argentine propolis extracts on streptococcus mutans and actinomyces viscosus
publisher Universidade Federal de Minas Gerais
publishDate 2020
url http://repositorio.unne.edu.ar/handle/123456789/1654
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AT juarezrolandopabloalejandro invitroantibacterialeffectofargentinepropolisextractsonstreptococcusmutansandactinomycesviscosus
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