Isolation and selection of potentially beneficial autochthonous bacteria for Piaractus mesopotamicus aquaculture activities

Piaractus mesopotamicus is the most produced specie in Argentinian fish culture with an annual production of 2,017 tons. The high demands of specimens generated a deficit of larvae, juveniles and sexually mature adults. The high stress conditions and the incidence of diseases had intensified this de...

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Autores principales: Guidoli, Marcos Gabriel, Santinón, Juan José, Pasteri, Sergio Enrique, Sánchez, Sebastián, Nader Macías, María Elena Fátima
Formato: Artículo
Lenguaje:Inglés
Publicado: Elsevier 2020
Materias:
Piaractus mesopotamicus
Beneficial bacterial strains
Probiotics
Aquaculture
Lactic acid bacteria
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/1542
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-1542
record_format dspace
spelling I48-R184-123456789-15422024-10-23T10:50:48Z Isolation and selection of potentially beneficial autochthonous bacteria for Piaractus mesopotamicus aquaculture activities Guidoli, Marcos Gabriel Santinón, Juan José Pasteri, Sergio Enrique Sánchez, Sebastián Nader Macías, María Elena Fátima Piaractus mesopotamicus Beneficial bacterial strains Probiotics Aquaculture Lactic acid bacteria Piaractus mesopotamicus is the most produced specie in Argentinian fish culture with an annual production of 2,017 tons. The high demands of specimens generated a deficit of larvae, juveniles and sexually mature adults. The high stress conditions and the incidence of diseases had intensified this deficit. Probiotics are proven to increase survival and growth rates in aquaculture. The first step in the design of a putative probiotic is the selection of adequate microorganisms to boost fish health. Lactic Acid Bacteria and Bacillus are generally accepted as probiotics in aquaculture. In this work we obtained 522 isolates from different anatomical parts of P. mesopotamicus, with a total of 30 lactic acid bacteria (LAB) and 8 Bacillus strains submitted for the evaluation of their beneficial properties. Only eight of these strains were selected for further “in vivo” assays based on following criteria: production of inhibitory metabolites and biosurfactants, screening of antagonistic activity against foodborne and pathogenic microorganisms, ability to emulsify polar solvents and compatibility between strains. All selected microorganisms were identified by sequencing 16S RNA gene. Pure cultures of all strains herein studied are preserved in Centro de Referencia de Lactobacilos (CERELA-CONICET) and Laboratorio de Sanidad Animal of the Estación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnología Agropecuaria (INTA) culture collections. Fil: Guidoli, Marcos Gabriel. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina. Fil: Santinón, Juan José. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina. Fil: Sánchez, Sebastián. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina. Fil: Nader Macías, María Elena Fátima. Universidad Nacional del Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. 2020-06-02T22:33:09Z 2020-06-02T22:33:09Z 2015 Artículo Guidoli, Marcos Gabriel, et al., 2015. Isolation and selection of potentially beneficial autochthonous bacteria for Piaractus mesopotamicus aquaculture activities. Journal Bioprocess Biotech. New York: Elsevier, vol. 5, no. 9, p. 1-10. ISSN 2155-9821. http://repositorio.unne.edu.ar/handle/123456789/1542 eng openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 1-10 application/pdf Elsevier Journal Bioprocess Biotech, 2015, vol. 5, no. 9, p. 1-10.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Piaractus mesopotamicus
Beneficial bacterial strains
Probiotics
Aquaculture
Lactic acid bacteria
spellingShingle Piaractus mesopotamicus
Beneficial bacterial strains
Probiotics
Aquaculture
Lactic acid bacteria
Guidoli, Marcos Gabriel
Santinón, Juan José
Pasteri, Sergio Enrique
Sánchez, Sebastián
Nader Macías, María Elena Fátima
Isolation and selection of potentially beneficial autochthonous bacteria for Piaractus mesopotamicus aquaculture activities
topic_facet Piaractus mesopotamicus
Beneficial bacterial strains
Probiotics
Aquaculture
Lactic acid bacteria
description Piaractus mesopotamicus is the most produced specie in Argentinian fish culture with an annual production of 2,017 tons. The high demands of specimens generated a deficit of larvae, juveniles and sexually mature adults. The high stress conditions and the incidence of diseases had intensified this deficit. Probiotics are proven to increase survival and growth rates in aquaculture. The first step in the design of a putative probiotic is the selection of adequate microorganisms to boost fish health. Lactic Acid Bacteria and Bacillus are generally accepted as probiotics in aquaculture. In this work we obtained 522 isolates from different anatomical parts of P. mesopotamicus, with a total of 30 lactic acid bacteria (LAB) and 8 Bacillus strains submitted for the evaluation of their beneficial properties. Only eight of these strains were selected for further “in vivo” assays based on following criteria: production of inhibitory metabolites and biosurfactants, screening of antagonistic activity against foodborne and pathogenic microorganisms, ability to emulsify polar solvents and compatibility between strains. All selected microorganisms were identified by sequencing 16S RNA gene. Pure cultures of all strains herein studied are preserved in Centro de Referencia de Lactobacilos (CERELA-CONICET) and Laboratorio de Sanidad Animal of the Estación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnología Agropecuaria (INTA) culture collections.
format Artículo
author Guidoli, Marcos Gabriel
Santinón, Juan José
Pasteri, Sergio Enrique
Sánchez, Sebastián
Nader Macías, María Elena Fátima
author_facet Guidoli, Marcos Gabriel
Santinón, Juan José
Pasteri, Sergio Enrique
Sánchez, Sebastián
Nader Macías, María Elena Fátima
author_sort Guidoli, Marcos Gabriel
title Isolation and selection of potentially beneficial autochthonous bacteria for Piaractus mesopotamicus aquaculture activities
title_short Isolation and selection of potentially beneficial autochthonous bacteria for Piaractus mesopotamicus aquaculture activities
title_full Isolation and selection of potentially beneficial autochthonous bacteria for Piaractus mesopotamicus aquaculture activities
title_fullStr Isolation and selection of potentially beneficial autochthonous bacteria for Piaractus mesopotamicus aquaculture activities
title_full_unstemmed Isolation and selection of potentially beneficial autochthonous bacteria for Piaractus mesopotamicus aquaculture activities
title_sort isolation and selection of potentially beneficial autochthonous bacteria for piaractus mesopotamicus aquaculture activities
publisher Elsevier
publishDate 2020
url http://repositorio.unne.edu.ar/handle/123456789/1542
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