Genotoxicity of pesticides: the role of non-active ingredients
Amitraz is a formamidine-based insecticide and acaricide used in veterinary applications. Despite the use of various genetic assessment criteria and testing systems to investigate amitraz poisoning, studies have yielded diverse and inconclusive results. This study aimed to analyze the genotoxic pote...
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Universidad Nacional del Nordeste
2025
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| Acceso en línea: | https://revistas.unne.edu.ar/index.php/vet/article/view/8111 |
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I48-R154-article-8111 |
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I48-R154-article-81112025-03-06T23:07:06Z Genotoxicity of pesticides: the role of non-active ingredients Genotoxicidad de pesticidas: el papel de los ingredientes no activos Pardiñas, C. Pellegrino, F. Padula, G. Seoane, A. Amitraz cell viability test genotoxicity quantitative comet assay micronucleus test cell cultures Amitraz viabilidad celular genotoxicidad ensayo cometa cuantitativo ensayo de micronúcleos cultivo celular Amitraz is a formamidine-based insecticide and acaricide used in veterinary applications. Despite the use of various genetic assessment criteria and testing systems to investigate amitraz poisoning, studies have yielded diverse and inconclusive results. This study aimed to analyze the genotoxic potential of the insecticide amitraz and compare the effects of the active constituent and a commercial product containing amitraz. Chinese hamster ovary cells were cultured during one cellular cycle in Ham F12 medium containing 1.25, 2.5 and 3.75 μg mL-1 of amitraz. Quantitative comet and cytokinesis-block micronucleus assay were employed to evaluate the potential genotoxic effect. All genotoxicity parameters evaluated clearly demonstrated the capability of the commercial amitraz formulation to negatively affect DNA, inducing both cytogenetic and cytomolecular damage. After culturing the cells with the active component of the formula, only a slight, non-significant increase in damage was found. Since our findings showed that the active component of the formula is not the sole responsible for the genotoxic effect of the commercial product, we emphasize the importance of considering the adverse effects of the solvents used in commercial pesticide formulations. Amitraz es un insecticida y acaricida a base de formamidina de uso veterinario. A pesar de que se han utilizado diversos criterios de valoración genéticos y sistemas de prueba para la investigación sobre el envenenamiento por amitraz, los resultados que surgieron de estas investigaciones fueron diversos y no concluyentes. El objetivo de este estudio fue analizar el potencial genotóxico del insecticida amitraz y comparar el efecto del componente activo y un producto comercial que contiene amitraz. Se cultivaron células de ovario de hámster chino durante un ciclo celular en medio Ham F12 con concentraciones de 1,25; 2,5 y 3,75 μg ml-1 de amitraz. Se emplearon los ensayos de micronúcleos con bloqueo de la citocinesis y cometa cuantitativo para evaluar el posible efecto genotóxico. Todos los parámetros de genotoxicidad evaluados mostraron claramente la capacidad de la formulación comercial de amitraz para afectar negativamente al ADN, induciendo daño tanto citogenético como citomolecular. Después de cultivar las células con el componente activo de la fórmula, solo se encontró un ligero aumento no significativo del daño. Como nuestros hallazgos demostraron que el componente activo de la fórmula no es el único responsable del efecto genotóxico del producto comercial, enfatizamos la importancia de tener en cuenta el efecto adverso de los solventes utilizados en las formulaciones comerciales de pesticidas. Universidad Nacional del Nordeste 2025-03-06 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/vet/article/view/8111 10.30972/vet.3618111 Revista Veterinaria; Vol. 36 Núm. 1 (2025); 1-6 1669-6840 1668-4834 eng https://revistas.unne.edu.ar/index.php/vet/article/view/8111/7690 https://creativecommons.org/licenses/by-nc/4.0 |
| institution |
Universidad Nacional del Nordeste |
| institution_str |
I-48 |
| repository_str |
R-154 |
| container_title_str |
Revistas UNNE - Universidad Nacional del Noroeste (UNNE) |
| language |
Inglés |
| format |
Artículo revista |
| topic |
Amitraz cell viability test genotoxicity quantitative comet assay micronucleus test cell cultures Amitraz viabilidad celular genotoxicidad ensayo cometa cuantitativo ensayo de micronúcleos cultivo celular |
| spellingShingle |
Amitraz cell viability test genotoxicity quantitative comet assay micronucleus test cell cultures Amitraz viabilidad celular genotoxicidad ensayo cometa cuantitativo ensayo de micronúcleos cultivo celular Pardiñas, C. Pellegrino, F. Padula, G. Seoane, A. Genotoxicity of pesticides: the role of non-active ingredients |
| topic_facet |
Amitraz cell viability test genotoxicity quantitative comet assay micronucleus test cell cultures Amitraz viabilidad celular genotoxicidad ensayo cometa cuantitativo ensayo de micronúcleos cultivo celular |
| author |
Pardiñas, C. Pellegrino, F. Padula, G. Seoane, A. |
| author_facet |
Pardiñas, C. Pellegrino, F. Padula, G. Seoane, A. |
| author_sort |
Pardiñas, C. |
| title |
Genotoxicity of pesticides: the role of non-active ingredients |
| title_short |
Genotoxicity of pesticides: the role of non-active ingredients |
| title_full |
Genotoxicity of pesticides: the role of non-active ingredients |
| title_fullStr |
Genotoxicity of pesticides: the role of non-active ingredients |
| title_full_unstemmed |
Genotoxicity of pesticides: the role of non-active ingredients |
| title_sort |
genotoxicity of pesticides: the role of non-active ingredients |
| description |
Amitraz is a formamidine-based insecticide and acaricide used in veterinary applications. Despite the use of various genetic assessment criteria and testing systems to investigate amitraz poisoning, studies have yielded diverse and inconclusive results. This study aimed to analyze the genotoxic potential of the insecticide amitraz and compare the effects of the active constituent and a commercial product containing amitraz. Chinese hamster ovary cells were cultured during one cellular cycle in Ham F12 medium containing 1.25, 2.5 and 3.75 μg mL-1 of amitraz. Quantitative comet and cytokinesis-block micronucleus assay were employed to evaluate the potential genotoxic effect. All genotoxicity parameters evaluated clearly demonstrated the capability of the commercial amitraz formulation to negatively affect DNA, inducing both cytogenetic and cytomolecular damage. After culturing the cells with the active component of the formula, only a slight, non-significant increase in damage was found. Since our findings showed that the active component of the formula is not the sole responsible for the genotoxic effect of the commercial product, we emphasize the importance of considering the adverse effects of the solvents used in commercial pesticide formulations. |
| publisher |
Universidad Nacional del Nordeste |
| publishDate |
2025 |
| url |
https://revistas.unne.edu.ar/index.php/vet/article/view/8111 |
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2025-05-17T05:10:42Z |
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2025-05-17T05:10:42Z |
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