Reduction of in vitro viral replication of caprine alphaherpesvirus 1 (CPHV-1) through natural antiviral treatment with plant extracts
Caprine herpesvirus 1 (CpHV-1) is distributed worldwide, causing significant economic losses. The application of acyclovir interferes with viral replication to varying degrees of efficacy, but it does not prevent the establishment of latency. Due to the costs and side effects associated with synthet...
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| Autores principales: | , , , , , , , , |
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Universidad Nacional del Nordeste
2025
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| Materias: | |
| Acceso en línea: | https://revistas.unne.edu.ar/index.php/vet/article/view/8088 |
| Aporte de: |
| id |
I48-R154-article-8088 |
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ojs |
| institution |
Universidad Nacional del Nordeste |
| institution_str |
I-48 |
| repository_str |
R-154 |
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Revistas UNNE - Universidad Nacional del Noroeste (UNNE) |
| language |
Español |
| format |
Artículo revista |
| topic |
herpesvius treatment L. divaricata M. verticillata P. quadrangularis herpesvius tratamiento L. divaricata M. verticillata P. quadrangularis |
| spellingShingle |
herpesvius treatment L. divaricata M. verticillata P. quadrangularis herpesvius tratamiento L. divaricata M. verticillata P. quadrangularis Ferreccio, C. Maidana, S. Tau, R. Di Ciaccio, L. Spotorno, V. Salvat, A. Aguilar, J. J. Konigheim, B. Romera, S. A. Reduction of in vitro viral replication of caprine alphaherpesvirus 1 (CPHV-1) through natural antiviral treatment with plant extracts |
| topic_facet |
herpesvius treatment L. divaricata M. verticillata P. quadrangularis herpesvius tratamiento L. divaricata M. verticillata P. quadrangularis |
| author |
Ferreccio, C. Maidana, S. Tau, R. Di Ciaccio, L. Spotorno, V. Salvat, A. Aguilar, J. J. Konigheim, B. Romera, S. A. |
| author_facet |
Ferreccio, C. Maidana, S. Tau, R. Di Ciaccio, L. Spotorno, V. Salvat, A. Aguilar, J. J. Konigheim, B. Romera, S. A. |
| author_sort |
Ferreccio, C. |
| title |
Reduction of in vitro viral replication of caprine alphaherpesvirus 1 (CPHV-1) through natural antiviral treatment with plant extracts |
| title_short |
Reduction of in vitro viral replication of caprine alphaherpesvirus 1 (CPHV-1) through natural antiviral treatment with plant extracts |
| title_full |
Reduction of in vitro viral replication of caprine alphaherpesvirus 1 (CPHV-1) through natural antiviral treatment with plant extracts |
| title_fullStr |
Reduction of in vitro viral replication of caprine alphaherpesvirus 1 (CPHV-1) through natural antiviral treatment with plant extracts |
| title_full_unstemmed |
Reduction of in vitro viral replication of caprine alphaherpesvirus 1 (CPHV-1) through natural antiviral treatment with plant extracts |
| title_sort |
reduction of in vitro viral replication of caprine alphaherpesvirus 1 (cphv-1) through natural antiviral treatment with plant extracts |
| description |
Caprine herpesvirus 1 (CpHV-1) is distributed worldwide, causing significant economic losses. The application of acyclovir interferes with viral replication to varying degrees of efficacy, but it does not prevent the establishment of latency. Due to the costs and side effects associated with synthetic drugs, the search for new chemotherapeutic agents is essential for the treatment and control of viral diseases. The objective of this study is to evaluate and compare the in vitro antiviral activity of three native plants from Argentina as a therapeutic antiviral treatment against CpHV-1. Larrea divaricata, Minthostachys verticillata and Parastrephia quadrangularis were used. Four parameters were analyzed: Cytotoxic Concentration 50 (CC50), Inhibitory Concentration 50 (IC50), quantification by plaque assay and viral titration. The L. divaricata extract showed the lowest cytotoxicity (2.4 mg ml-1). The best selectivity index (SI) was obtained with the M. verticillata extract (10). L. divaricata and P. quadrangularis extracts demonstrated the greatest antiviral effects, with reductions of 90% and 75%, respectively, compared to untreated controls, where the greatest reduction in plaque size was observed. The highest inhibition of viral replication observed in the viral titration quantification occurred when monolayers were treated with L. divaricata, resulting in at least a 35% reduction. The combination of assay with L. divaricata and M. verticillata showed the greatest reduction in viral titers. The overall results provide evidence that L. divaricata and M. verticillata could serve as potential sources for new anti-CpHV-1 drugs. |
| publisher |
Universidad Nacional del Nordeste |
| publishDate |
2025 |
| url |
https://revistas.unne.edu.ar/index.php/vet/article/view/8088 |
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I48-R154-article-80882025-02-12T21:58:12Z Reduction of in vitro viral replication of caprine alphaherpesvirus 1 (CPHV-1) through natural antiviral treatment with plant extracts Reducción de la replicación viral in vitro de alfaherpesvirus caprino 1 (CpHV-1) por tratamiento antiviral natural de extractos de plantas Ferreccio, C. Maidana, S. Tau, R. Di Ciaccio, L. Spotorno, V. Salvat, A. Aguilar, J. J. Konigheim, B. Romera, S. A. herpesvius treatment L. divaricata M. verticillata P. quadrangularis herpesvius tratamiento L. divaricata M. verticillata P. quadrangularis Caprine herpesvirus 1 (CpHV-1) is distributed worldwide, causing significant economic losses. The application of acyclovir interferes with viral replication to varying degrees of efficacy, but it does not prevent the establishment of latency. Due to the costs and side effects associated with synthetic drugs, the search for new chemotherapeutic agents is essential for the treatment and control of viral diseases. The objective of this study is to evaluate and compare the in vitro antiviral activity of three native plants from Argentina as a therapeutic antiviral treatment against CpHV-1. Larrea divaricata, Minthostachys verticillata and Parastrephia quadrangularis were used. Four parameters were analyzed: Cytotoxic Concentration 50 (CC50), Inhibitory Concentration 50 (IC50), quantification by plaque assay and viral titration. The L. divaricata extract showed the lowest cytotoxicity (2.4 mg ml-1). The best selectivity index (SI) was obtained with the M. verticillata extract (10). L. divaricata and P. quadrangularis extracts demonstrated the greatest antiviral effects, with reductions of 90% and 75%, respectively, compared to untreated controls, where the greatest reduction in plaque size was observed. The highest inhibition of viral replication observed in the viral titration quantification occurred when monolayers were treated with L. divaricata, resulting in at least a 35% reduction. The combination of assay with L. divaricata and M. verticillata showed the greatest reduction in viral titers. The overall results provide evidence that L. divaricata and M. verticillata could serve as potential sources for new anti-CpHV-1 drugs. El herpesvirus caprino 1 (CpHV-1) se distribuye mundialmente siendo responsable de pérdidas económicas. La aplicación de aciclovir interfiere en la replicación viral con distintos grados de eficacia, pero no impide el establecimiento de latencia. Debido a los costos y efectos secundarios que producen los fármacos antihipertensivos, la búsqueda de nuevos agentes quimioterapéuticos es fundamental para el tratamiento y control de las enfermedades virales. El objetivo del trabajo es evaluar y comparar la actividad antiviral in vitro de tres plantas nativas de Argentina como tratamiento antiviral terapéutico contra CpHV-1. Se utilizaron Larrea divaricata, Minthostachys verticillata y Parastrephia quadrangularis. Se analizaron cuatro parámetros: Concentración Citotóxica 50 (CC50), Concentración Inhibitoria 50 (CI50), cuantificación por ensayo de placa y titulación viral. El extracto de L. divaricata mostró la menor citotoxicidad (2,4 mg ml-1). El mejor SI (índice de selectividad) se obtuvo en el extracto de M. verticillata (10). Los extractos de L. divaricata y P. quadrangularis son los compuestos que mostraron el mayor efecto antiviral (90% y 75%, respectivamente), en comparación con los controles no tratados, en los que se observó la mayor reducción en el tamaño de la placa. La mayor inhibición de la replicación viral se observó cuando se evaluó el título viral cuando las monocapas fueron tratadas con L. divaricata (al menos disminución del 35%). Con el ensayo de combinación de L. divaricata y M. verticillata se observó la mayor reducción de los títulos virales. Los resultados generales proporcionan evidencia de que L. divaricata y M. verticillata podrían ser fuentes potenciales de nuevos fármacos anti-CpHV-1. Universidad Nacional del Nordeste 2025-02-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/vet/article/view/8088 10.30972/vet.3618088 Revista Veterinaria; Vol. 36 Núm. 1 (2025); 1-8 1669-6840 1668-4834 spa https://revistas.unne.edu.ar/index.php/vet/article/view/8088/7622 https://creativecommons.org/licenses/by-nc/4.0 |