Structuring a BALB/c mice colony in the Unidad Experimental de Inmunoterapia of Fundación Jacinto Convit – Venezuela
Animal models are a fundamental part of preclinical scientific research in the development of new therapies including drugs and gene therapies. For such purposes, it is crucial to have an animal model that is appropriate for specific study. At the animal facility of the Unidad Experimental de Inmuno...
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Universidad Nacional del Nordeste
2025
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| Acceso en línea: | https://revistas.unne.edu.ar/index.php/vet/article/view/7988 |
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I48-R154-article-7988 |
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Universidad Nacional del Nordeste |
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I-48 |
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R-154 |
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Revistas UNNE - Universidad Nacional del Noroeste (UNNE) |
| language |
Español |
| format |
Artículo revista |
| topic |
mice colony strain purity microsatellite analysis homozygosity mixed animal facility colonia de ratones pureza de cepa análisis de microsatélites homocigosis bioterio mixto |
| spellingShingle |
mice colony strain purity microsatellite analysis homozygosity mixed animal facility colonia de ratones pureza de cepa análisis de microsatélites homocigosis bioterio mixto Duarte, M. A. Navarro, E. Carballo, J. Negrón, E. Blanca, I. De Jesús, R. Rivodó, T. Aranguren, D. Convit, A. F. Structuring a BALB/c mice colony in the Unidad Experimental de Inmunoterapia of Fundación Jacinto Convit – Venezuela |
| topic_facet |
mice colony strain purity microsatellite analysis homozygosity mixed animal facility colonia de ratones pureza de cepa análisis de microsatélites homocigosis bioterio mixto |
| author |
Duarte, M. A. Navarro, E. Carballo, J. Negrón, E. Blanca, I. De Jesús, R. Rivodó, T. Aranguren, D. Convit, A. F. |
| author_facet |
Duarte, M. A. Navarro, E. Carballo, J. Negrón, E. Blanca, I. De Jesús, R. Rivodó, T. Aranguren, D. Convit, A. F. |
| author_sort |
Duarte, M. A. |
| title |
Structuring a BALB/c mice colony in the Unidad Experimental de Inmunoterapia of Fundación Jacinto Convit – Venezuela |
| title_short |
Structuring a BALB/c mice colony in the Unidad Experimental de Inmunoterapia of Fundación Jacinto Convit – Venezuela |
| title_full |
Structuring a BALB/c mice colony in the Unidad Experimental de Inmunoterapia of Fundación Jacinto Convit – Venezuela |
| title_fullStr |
Structuring a BALB/c mice colony in the Unidad Experimental de Inmunoterapia of Fundación Jacinto Convit – Venezuela |
| title_full_unstemmed |
Structuring a BALB/c mice colony in the Unidad Experimental de Inmunoterapia of Fundación Jacinto Convit – Venezuela |
| title_sort |
structuring a balb/c mice colony in the unidad experimental de inmunoterapia of fundación jacinto convit – venezuela |
| description |
Animal models are a fundamental part of preclinical scientific research in the development of new therapies including drugs and gene therapies. For such purposes, it is crucial to have an animal model that is appropriate for specific study. At the animal facility of the Unidad Experimental de Inmunoterapia (UEI) of Fundación Jacinto Convit (FJC), a colony of BALB/c mice was established to maintain a pure strain for experimental research projects in breast cancer, using the 4T1 autologous tumor cell line. The colony was established from two pairs of BALB/c mice provided by Universidad Simón Bolívar, ensuring strict housing conditions in micro-isolators, with appropriate feeding, environmental enrichment, and detailed control of both micro and macroenvironment parameters required for the species. Animals care was managed by a specialized biologist. The expansion of this BALB/c mouse strain was monitored following a pattern of inbred crosses every third litter. Homozygosity was determined by using skin grafting technique among individuals of the same strain and comparative microsatellite analysis. Data from 135 births were analyzed, with a maximum of 6 births per breeding box. Monogamous pairs produced an average of 6.25 ± 2.38 pups per litter, with no unexpected deaths, and an interbirth interval of 27 ± 7.5 days. The overall results obtained in this work demonstrated that the breeding, care, and maintenance protocol of the BALB/c mouse colony at the UEI animal facility of FJC is effective in ensuring the expansion and genetic purity of this strain, hereafter referred to as BALB/c//USB//FJC. |
| publisher |
Universidad Nacional del Nordeste |
| publishDate |
2025 |
| url |
https://revistas.unne.edu.ar/index.php/vet/article/view/7988 |
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I48-R154-article-79882025-02-11T14:37:16Z Structuring a BALB/c mice colony in the Unidad Experimental de Inmunoterapia of Fundación Jacinto Convit – Venezuela Estructuración de una colonia de ratones BALB/c en la Unidad Experimental de Inmunoterapia de la Fundación Jacinto Convit – Venezuela Duarte, M. A. Navarro, E. Carballo, J. Negrón, E. Blanca, I. De Jesús, R. Rivodó, T. Aranguren, D. Convit, A. F. mice colony strain purity microsatellite analysis homozygosity mixed animal facility colonia de ratones pureza de cepa análisis de microsatélites homocigosis bioterio mixto Animal models are a fundamental part of preclinical scientific research in the development of new therapies including drugs and gene therapies. For such purposes, it is crucial to have an animal model that is appropriate for specific study. At the animal facility of the Unidad Experimental de Inmunoterapia (UEI) of Fundación Jacinto Convit (FJC), a colony of BALB/c mice was established to maintain a pure strain for experimental research projects in breast cancer, using the 4T1 autologous tumor cell line. The colony was established from two pairs of BALB/c mice provided by Universidad Simón Bolívar, ensuring strict housing conditions in micro-isolators, with appropriate feeding, environmental enrichment, and detailed control of both micro and macroenvironment parameters required for the species. Animals care was managed by a specialized biologist. The expansion of this BALB/c mouse strain was monitored following a pattern of inbred crosses every third litter. Homozygosity was determined by using skin grafting technique among individuals of the same strain and comparative microsatellite analysis. Data from 135 births were analyzed, with a maximum of 6 births per breeding box. Monogamous pairs produced an average of 6.25 ± 2.38 pups per litter, with no unexpected deaths, and an interbirth interval of 27 ± 7.5 days. The overall results obtained in this work demonstrated that the breeding, care, and maintenance protocol of the BALB/c mouse colony at the UEI animal facility of FJC is effective in ensuring the expansion and genetic purity of this strain, hereafter referred to as BALB/c//USB//FJC. Los modelos animales son parte fundamental de la investigación científica preclínica en el desarrollo de nuevas terapias, incluyendo drogas y terapias génicas. Para tales fines, es crucial contar con un modelo animal acorde con el estudio a realizar. En el bioterio de la Unidad Experimental de Inmunoterapia (UEI) de la Fundación Jacinto Convit (FJC) se estructuró una colonia de ratones BALB/c a fin de contar con una cepa pura para llevar a cabo proyectos de investigación experimental en cáncer de mama, utilizando la línea de células de tumor autólogo 4T1. Esta colonia se estableció a partir de dos parejas de ratones BALB/c proporcionados por la Universidad Simón Bolívar, asegurando condiciones estrictas de alojamiento en microaisladores, con alimentación adecuada, enriquecimiento ambiental y control detallado del micro y macroambiente requerido para la especie. El cuidado de los animales estuvo a cargo de un biólogo especializado. La expansión de esta cepa de ratones BALB/c se evaluó siguiendo un esquema de cruces endogámicos programados en cada tercer parto. La homocigosidad se determinó mediante la técnica de injerto de piel entre individuos de la misma cepa y el análisis comparativo de microsatélites. Se analizaron los datos obtenidos de 135 partos, con un máximo de 6 partos por caja reproductora, provenientes de parejas monógamas donde no ocurrieron muertes inesperadas y se obtuvo en promedio de 6,25 ± 2,38 crías por parto, y un periodo interparto de 27 ± 7,5 días. El conjunto de los resultados obtenidos en este trabajo demostró que el protocolo de reproducción, cuidado y mantenimiento de la colonia de ratones BALB/c en el bioterio de la UEI de la FJC, es eficaz para garantizar la expansión y pureza genética de esta cepa, en adelante denominada BALB/c//USB//FJC. Universidad Nacional del Nordeste 2025-01-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/vet/article/view/7988 10.30972/vet.3617988 Revista Veterinaria; Vol. 36 Núm. 1 (2025); 1-10 1669-6840 1668-4834 spa https://revistas.unne.edu.ar/index.php/vet/article/view/7988/7488 https://creativecommons.org/licenses/by-nc/4.0 |