Efficacy of SNAP DUO ST Plus and HPLC in the detection of antibiotic residues in raw milk from the Mantaro Valley, Peru
The objective of the present study was to determine and compare the efficacy of SNAP DUO ST Plus and HPLC in the detection of antibiotic residues in milk produced to ensure food safety. To this end, raw milk samples from 32 dairy herds and 8 collection centers were evaluated using the SNAP DUO* ST P...
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| Autores principales: | , , , , , , |
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| Formato: | Artículo revista |
| Lenguaje: | Español |
| Publicado: |
Universidad Nacional del Nordeste
2025
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| Materias: | |
| Acceso en línea: | https://revistas.unne.edu.ar/index.php/vet/article/view/7987 |
| Aporte de: |
| Sumario: | The objective of the present study was to determine and compare the efficacy of SNAP DUO ST Plus and HPLC in the detection of antibiotic residues in milk produced to ensure food safety. To this end, raw milk samples from 32 dairy herds and 8 collection centers were evaluated using the SNAP DUO* ST Plus rapid test for tetracyclines and β-lactams, and the high-performance liquid chromatography (HPLC) method. The analysis was conducted in laboratories at the National University of the Center of Peru. A total of 80 milk samples were analyzed, with antibiotic residues found in 37.5% of the samples using SNAP DUO, and in 52.5% using HPLC. The SNAP DUO test showed substantial agreement with HPLC (Kappa=0.734), with a sensitivity and specificity of 89.3% and 95.3%, respectively, compared to 100% for HPLC. In terms of cost-effectiveness, HPLC presented a higher cost per correctly identified result (S/ 20.27) compared to SNAP DUO (S/ 7.24), which was identified as the most efficient method. The implementation of SNAP DUO in antimicrobial residue monitoring programs in milk is recommended due to its effectiveness and efficiency, while HPLC is ideal for research and corroboration of results, contributing to the improvement of food safety in milk consumption. |
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