Efficacy of SNAP DUO ST Plus and HPLC in the detection of antibiotic residues in raw milk from the Mantaro Valley, Peru

The objective of the present study was to determine and compare the efficacy of SNAP DUO ST Plus and HPLC in the detection of antibiotic residues in milk produced to ensure food safety. To this end, raw milk samples from 32 dairy herds and 8 collection centers were evaluated using the SNAP DUO* ST P...

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Detalles Bibliográficos
Autores principales: Villar, F. A., Estremadoyro, L. G., Payano, I. U., Sanchez, N. M., Laurente, K. A., Maurico-Ramos, Y., Carhuas, J. N.
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional del Nordeste 2025
Materias:
Milk diagnostic tests
Rapid screening
Sensitivity
Specificity
Cost- effectiveness
Pruebas diagnósticas leche
Screening rápido
Sensibilidad
Especificidad
Costo–efectividad
Acceso en línea:https://revistas.unne.edu.ar/index.php/vet/article/view/7987
Aporte de:
Revistas UNNE - Universidad Nacional del Noroeste (UNNE) de Universidad Nacional del Nordeste
id I48-R154-article-7987
record_format ojs
spelling I48-R154-article-79872025-02-11T14:37:16Z Efficacy of SNAP DUO ST Plus and HPLC in the detection of antibiotic residues in raw milk from the Mantaro Valley, Peru Eficacia de SNAP DUO ST Plus y HPLC en la detección de residuos de antibióticos en leche cruda del Valle del Mantaro, Perú Villar, F. A. Estremadoyro, L. G. Payano, I. U. Sanchez, N. M. Laurente, K. A. Maurico-Ramos, Y. Carhuas, J. N. Milk diagnostic tests Rapid screening Sensitivity Specificity Cost- effectiveness Pruebas diagnósticas leche Screening rápido Sensibilidad Especificidad Costo–efectividad The objective of the present study was to determine and compare the efficacy of SNAP DUO ST Plus and HPLC in the detection of antibiotic residues in milk produced to ensure food safety. To this end, raw milk samples from 32 dairy herds and 8 collection centers were evaluated using the SNAP DUO* ST Plus rapid test for tetracyclines and β-lactams, and the high-performance liquid chromatography (HPLC) method. The analysis was conducted in laboratories at the National University of the Center of Peru. A total of 80 milk samples were analyzed, with antibiotic residues found in 37.5% of the samples using SNAP DUO, and in 52.5% using HPLC. The SNAP DUO test showed substantial agreement with HPLC (Kappa=0.734), with a sensitivity and specificity of 89.3% and 95.3%, respectively, compared to 100% for HPLC. In terms of cost-effectiveness, HPLC presented a higher cost per correctly identified result (S/ 20.27) compared to SNAP DUO (S/ 7.24), which was identified as the most efficient method. The implementation of SNAP DUO in antimicrobial residue monitoring programs in milk is recommended due to its effectiveness and efficiency, while HPLC is ideal for research and corroboration of results, contributing to the improvement of food safety in milk consumption. El objetivo del presente estudio fue determinar y comparar la eficacia de SNAP DUO ST Plus y HPLC en la detección de residuos de antibióticos en la leche producida para garantizar la inocuidad alimentaria. Con este objetivo, se evaluaron muestras de leche cruda de 32 hatos lecheros y 8 centros de acopio utilizando la prueba rápida SNAP DUO* ST Plus para detectar tetraciclinas y β-lactámicos, y el método de cromatografía líquida de alta resolución (HPLC). El análisis se realizó en laboratorios de la Universidad Nacional del Centro del Perú. Se analizaron 80 muestras de leche, encontrándose residuos de antibióticos en el 37,5% de las muestras mediante SNAP DUO, y en el 52,5% mediante HPLC. La prueba SNAP DUO mostró una concordancia sustancial con HPLC (Kappa=0,734), con sensibilidad y especificidad del 89,3% y 95,3%, respectivamente, frente al 100% de HPLC. En términos de costo-efectividad, HPLC presentó un mayor costo por resultado correctamente identificado (S/ 20,27), en comparación con SNAP DUO (S/ 7,24), que fue identificado como el método más eficiente. Se recomienda la implementación de SNAP DUO en programas de monitoreo de residuos de antimicrobianos en leche por su efectividad y eficiencia, mientras que HPLC es ideal para investigación y corroboración de resultados, contribuyendo a la mejora de la inocuidad alimentaria en el consumo de leche. Universidad Nacional del Nordeste 2025-01-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/vet/article/view/7987 10.30972/vet.3617987 Revista Veterinaria; Vol. 36 Núm. 1 (2025); 1-7 1669-6840 1668-4834 spa https://revistas.unne.edu.ar/index.php/vet/article/view/7987/7487 https://creativecommons.org/licenses/by-nc/4.0
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-154
container_title_str Revistas UNNE - Universidad Nacional del Noroeste (UNNE)
language Español
format Artículo revista
topic Milk diagnostic tests
Rapid screening
Sensitivity
Specificity
Cost- effectiveness
Pruebas diagnósticas leche
Screening rápido
Sensibilidad
Especificidad
Costo–efectividad
spellingShingle Milk diagnostic tests
Rapid screening
Sensitivity
Specificity
Cost- effectiveness
Pruebas diagnósticas leche
Screening rápido
Sensibilidad
Especificidad
Costo–efectividad
Villar, F. A.
Estremadoyro, L. G.
Payano, I. U.
Sanchez, N. M.
Laurente, K. A.
Maurico-Ramos, Y.
Carhuas, J. N.
Efficacy of SNAP DUO ST Plus and HPLC in the detection of antibiotic residues in raw milk from the Mantaro Valley, Peru
topic_facet Milk diagnostic tests
Rapid screening
Sensitivity
Specificity
Cost- effectiveness
Pruebas diagnósticas leche
Screening rápido
Sensibilidad
Especificidad
Costo–efectividad
author Villar, F. A.
Estremadoyro, L. G.
Payano, I. U.
Sanchez, N. M.
Laurente, K. A.
Maurico-Ramos, Y.
Carhuas, J. N.
author_facet Villar, F. A.
Estremadoyro, L. G.
Payano, I. U.
Sanchez, N. M.
Laurente, K. A.
Maurico-Ramos, Y.
Carhuas, J. N.
author_sort Villar, F. A.
title Efficacy of SNAP DUO ST Plus and HPLC in the detection of antibiotic residues in raw milk from the Mantaro Valley, Peru
title_short Efficacy of SNAP DUO ST Plus and HPLC in the detection of antibiotic residues in raw milk from the Mantaro Valley, Peru
title_full Efficacy of SNAP DUO ST Plus and HPLC in the detection of antibiotic residues in raw milk from the Mantaro Valley, Peru
title_fullStr Efficacy of SNAP DUO ST Plus and HPLC in the detection of antibiotic residues in raw milk from the Mantaro Valley, Peru
title_full_unstemmed Efficacy of SNAP DUO ST Plus and HPLC in the detection of antibiotic residues in raw milk from the Mantaro Valley, Peru
title_sort efficacy of snap duo st plus and hplc in the detection of antibiotic residues in raw milk from the mantaro valley, peru
description The objective of the present study was to determine and compare the efficacy of SNAP DUO ST Plus and HPLC in the detection of antibiotic residues in milk produced to ensure food safety. To this end, raw milk samples from 32 dairy herds and 8 collection centers were evaluated using the SNAP DUO* ST Plus rapid test for tetracyclines and β-lactams, and the high-performance liquid chromatography (HPLC) method. The analysis was conducted in laboratories at the National University of the Center of Peru. A total of 80 milk samples were analyzed, with antibiotic residues found in 37.5% of the samples using SNAP DUO, and in 52.5% using HPLC. The SNAP DUO test showed substantial agreement with HPLC (Kappa=0.734), with a sensitivity and specificity of 89.3% and 95.3%, respectively, compared to 100% for HPLC. In terms of cost-effectiveness, HPLC presented a higher cost per correctly identified result (S/ 20.27) compared to SNAP DUO (S/ 7.24), which was identified as the most efficient method. The implementation of SNAP DUO in antimicrobial residue monitoring programs in milk is recommended due to its effectiveness and efficiency, while HPLC is ideal for research and corroboration of results, contributing to the improvement of food safety in milk consumption.
publisher Universidad Nacional del Nordeste
publishDate 2025
url https://revistas.unne.edu.ar/index.php/vet/article/view/7987
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first_indexed 2025-05-17T05:10:37Z
last_indexed 2025-05-17T05:10:37Z
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