Domestic bovines as potential environmental bioindicators: analysis of oral epithelium and application in the micronucleus assay
Domestic cattle (Bos Taurus) could be used as bioindicators of the quality of agroecosystems, with the possibility of alerting through cellular biomarkers about possible adverse effects of drugs administered in them or toxic contaminants in the surrounding environment. The micronucleus buccal cytome...
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Universidad Nacional del Nordeste
2024
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| Acceso en línea: | https://revistas.unne.edu.ar/index.php/vet/article/view/7859 |
| Aporte de: |
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I48-R154-article-7859 |
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ojs |
| institution |
Universidad Nacional del Nordeste |
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I-48 |
| repository_str |
R-154 |
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Revistas UNNE - Universidad Nacional del Noroeste (UNNE) |
| language |
Español |
| format |
Artículo revista |
| topic |
Agroecosystem Biomarker Exfoliated cells Histology Lining tissue Cattle Ecosistema agropecuario Biomarcador Células exfoliadas Histología Tejido de revestimiento Ganado |
| spellingShingle |
Agroecosystem Biomarker Exfoliated cells Histology Lining tissue Cattle Ecosistema agropecuario Biomarcador Células exfoliadas Histología Tejido de revestimiento Ganado Ferré, D.M Carracedo, R.T. Pedrosa, A. Caliri, M.N. Ludueña, H.R. Gorla, N.B. Domestic bovines as potential environmental bioindicators: analysis of oral epithelium and application in the micronucleus assay |
| topic_facet |
Agroecosystem Biomarker Exfoliated cells Histology Lining tissue Cattle Ecosistema agropecuario Biomarcador Células exfoliadas Histología Tejido de revestimiento Ganado |
| author |
Ferré, D.M Carracedo, R.T. Pedrosa, A. Caliri, M.N. Ludueña, H.R. Gorla, N.B. |
| author_facet |
Ferré, D.M Carracedo, R.T. Pedrosa, A. Caliri, M.N. Ludueña, H.R. Gorla, N.B. |
| author_sort |
Ferré, D.M |
| title |
Domestic bovines as potential environmental bioindicators: analysis of oral epithelium and application in the micronucleus assay |
| title_short |
Domestic bovines as potential environmental bioindicators: analysis of oral epithelium and application in the micronucleus assay |
| title_full |
Domestic bovines as potential environmental bioindicators: analysis of oral epithelium and application in the micronucleus assay |
| title_fullStr |
Domestic bovines as potential environmental bioindicators: analysis of oral epithelium and application in the micronucleus assay |
| title_full_unstemmed |
Domestic bovines as potential environmental bioindicators: analysis of oral epithelium and application in the micronucleus assay |
| title_sort |
domestic bovines as potential environmental bioindicators: analysis of oral epithelium and application in the micronucleus assay |
| description |
Domestic cattle (Bos Taurus) could be used as bioindicators of the quality of agroecosystems, with the possibility of alerting through cellular biomarkers about possible adverse effects of drugs administered in them or toxic contaminants in the surrounding environment. The micronucleus buccal cytome (MN-cyt buccal) assay is used in human populations for this purpose. The aim of this study was to perform the structural characterization of the epithelium in the anatomical site proposed for performing the oral MN-cyt buccal assay in this species and to describe the types and frequencies of cells with nuclear abnormalities (NA) of the bovine oral lining epithelium. Exfoliative cytology of the buccal labial epithelium of twelve castrated males was performed and 1,000 cells per animal were analyzed. The frequencies of basal and differentiated cells with NAs were established. The most frequently observed cell types and NAs were: karyolytic, condensed chromatin, karyorrhexis, pyknotic, kidney-shaped, notched nuclei, binucleated, micronucleated and buds. Four grades of progression were described in nuclei with karyorrhexis. A keratinized flat stratified epithelium of 866.67±75.44 μm thick (Mean ± SD) was evidenced and the characteristics of the cells of the strata germinativum, granulosum, spinosum and corneum are delineated. In addition to being keratinized, the bovine epithelium is three to five times thicker than that recorded in humans due to more differentiated cells. In the prospective use of buccal MN-cyt in bovines, indicators of cell death should not be considered as a result of genotoxic effects that induce apoptosis, as occurs in humans; the rest of the NAs could be used as biomarkers. |
| publisher |
Universidad Nacional del Nordeste |
| publishDate |
2024 |
| url |
https://revistas.unne.edu.ar/index.php/vet/article/view/7859 |
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2025-05-17T05:10:31Z |
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2025-05-17T05:10:31Z |
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I48-R154-article-78592024-11-01T13:19:27Z Domestic bovines as potential environmental bioindicators: analysis of oral epithelium and application in the micronucleus assay Los bovinos domésticos como potenciales bioindicadores ambientales: análisis del epitelio oral y aplicación en el ensayo de micronúcleos Ferré, D.M Carracedo, R.T. Pedrosa, A. Caliri, M.N. Ludueña, H.R. Gorla, N.B. Agroecosystem Biomarker Exfoliated cells Histology Lining tissue Cattle Ecosistema agropecuario Biomarcador Células exfoliadas Histología Tejido de revestimiento Ganado Domestic cattle (Bos Taurus) could be used as bioindicators of the quality of agroecosystems, with the possibility of alerting through cellular biomarkers about possible adverse effects of drugs administered in them or toxic contaminants in the surrounding environment. The micronucleus buccal cytome (MN-cyt buccal) assay is used in human populations for this purpose. The aim of this study was to perform the structural characterization of the epithelium in the anatomical site proposed for performing the oral MN-cyt buccal assay in this species and to describe the types and frequencies of cells with nuclear abnormalities (NA) of the bovine oral lining epithelium. Exfoliative cytology of the buccal labial epithelium of twelve castrated males was performed and 1,000 cells per animal were analyzed. The frequencies of basal and differentiated cells with NAs were established. The most frequently observed cell types and NAs were: karyolytic, condensed chromatin, karyorrhexis, pyknotic, kidney-shaped, notched nuclei, binucleated, micronucleated and buds. Four grades of progression were described in nuclei with karyorrhexis. A keratinized flat stratified epithelium of 866.67±75.44 μm thick (Mean ± SD) was evidenced and the characteristics of the cells of the strata germinativum, granulosum, spinosum and corneum are delineated. In addition to being keratinized, the bovine epithelium is three to five times thicker than that recorded in humans due to more differentiated cells. In the prospective use of buccal MN-cyt in bovines, indicators of cell death should not be considered as a result of genotoxic effects that induce apoptosis, as occurs in humans; the rest of the NAs could be used as biomarkers. Los bovinos domésticos (Bos Taurus) podrían utilizarse como bioindicadores de la calidad de los agroecosistemas, con la posibilidad de alertar mediante biomarcadores celulares sobre posibles efectos adversos de fármacos administrados en ellos o de contaminantes tóxicos en el ambiente circundante. El ensayo de micronúcleos citoma bucal (MN-cit bucal) se utiliza en poblaciones humanas con este fin. El objetivo de este estudio fue realizar la caracterización estructural del epitelio en el sitio anatómico propuesto para la realización del test MN-cit bucal en esta especie y describir los tipos y frecuencias de células con anormalidades nucleares (AN) del epitelio bucal bovino. Además, se realizó citología exfoliativa del epitelio bucal labial de doce machos castrados y se analizaron 1.000 células por animal, estableciéndose frecuencia de células basales y diferenciadas con AN. Los tipos celulares y AN observados con mayor frecuencia fueron: cariolíticas, cromatina condensada, cariorrexis, picnóticos, en forma de riñón, núcleos con muescas, binucleados, micronucleados y brotes. Se describieron cuatro grados de progresión en núcleos con cariorrexis. Se evidenció un epitelio estratificado plano queratinizado de 866,67 ± 75,44 μm de espesor (media ± DE) y se delinean las características de las células de los estratos germinativum, granulosum, spinosum y corneum. Además de estar queratinizado, el epitelio bovino es entre tres y cinco veces más grueso que el registrado en humanos debido a un número mayor de células diferenciadas. En el uso prospectivo de MN-cit bucal en bovinos, los indicadores de muerte celular no deben considerarse como resultado de efectos genotóxicos que inducen apoptosis, como ocurre en humanos; el resto de las AN si pueden ser utilizados como biomarcadores. Universidad Nacional del Nordeste 2024-11-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/vet/article/view/7859 10.30972/vet.3527859 Revista Veterinaria; Vol. 35 Núm. 2 (2024); 22-29 1669-6840 1668-4834 spa https://revistas.unne.edu.ar/index.php/vet/article/view/7859/7381 https://creativecommons.org/licenses/by-nc/4.0 |