Estimating attrition of Sprague Dawley rats for research involving primary neuronal cultures

The use of laboratory animals for biomedical research is strictly regulated to prevent their suffering and inadequate use. While experimentation is in process, attrition usually can increase the number of animals needed to achieve the objectives. Unfortunately, attrition is not commonly reported in...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Denis, B., De Jesus, R., Gittens, R.A
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional del Nordeste 2024
Materias:
Laboratory animals
Rat embryos
Sample size
Statistical analysis
Animales de laboratorio
Embriones de rata
Cálculo de muestra
Análisis estadístico
Acceso en línea:https://revistas.unne.edu.ar/index.php/vet/article/view/7858
Aporte de:
Revistas UNNE - Universidad Nacional del Noroeste (UNNE) de Universidad Nacional del Nordeste
id I48-R154-article-7858
record_format ojs
spelling I48-R154-article-78582024-11-01T13:19:27Z Estimating attrition of Sprague Dawley rats for research involving primary neuronal cultures Estimación de incidencias en ratas Sprague Dawley en investigaciones que involucren cultivos neuronales primarios Denis, B. De Jesus, R. Gittens, R.A Laboratory animals Rat embryos Sample size Statistical analysis Animales de laboratorio Embriones de rata Cálculo de muestra Análisis estadístico The use of laboratory animals for biomedical research is strictly regulated to prevent their suffering and inadequate use. While experimentation is in process, attrition usually can increase the number of animals needed to achieve the objectives. Unfortunately, attrition is not commonly reported in literature. In the present study, we worked with primary neuronal cell cultures to establish the first in vitro brain ischemia model in Panama. We report the effects of attrition in the final number of animals. Primary cortical cell cultures were produced from Sprague Dawley rat embryos. Vaginal cytology was used to evaluate oestrus cycle. Pregnant rats had routine evaluation plus clinical/weight follow up. Sample size calculations estimated 26 rats for the study using an attrition rate of 25%. The final number of animals used empirically was 26; however, the measured attrition was 50% (13). These results highlight the importance of calculating attrition for studies requiring laboratory animals, taking in consideration the context and available data from local institutions to allow for the most efficient use of laboratory animals, especially for newly established laboratories in developing countries. El uso de animales de laboratorio para investigación biomédica esta estrictamente regulado para prevenir su sufrimiento y uso inadecuado. Mientras que la experimentación está en proceso, las incidencias usualmente pueden incrementar el número de animales necesarios para alcanzar los objetivos. Desafortunadamente, las incidencias no son reportadas comúnmente en la literatura. En el presente estudio, trabajamos con cultivos neuronales primarios para establecer el primer modelo de isquemia cerebral in vitro en Panamá, reportando los efectos de las incidencias en el número final de animales. Los cultivos neuronales primarios fueron producidos de embriones de ratas Sprague Dawley. La citología vaginal fue utilizada para evaluar el ciclo estral. Las ratas gestantes además de sus evaluaciones de rutina recibieron seguimiento clínico y de peso. El cálculo de la muestra estimó la necesidad de 26 ratas para el estudio, utilizando una tasa de incidencias del 25%. El número final de animales utilizado empíricamente fue 26; sin embargo, la incidencia observada fue de 50% (13). Estos resultados subrayan la importancia del cálculo de incidencias para estudios que requieren animales de laboratorio, debiendo tomar en consideración el contexto y la información disponible de instituciones locales para permitir el uso más eficiente de los animales, en especial para laboratorios recientemente establecidos de países en vías de desarrollo. Universidad Nacional del Nordeste 2024-11-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/vet/article/view/7858 10.30972/vet.3527858 Revista Veterinaria; Vol. 35 Núm. 2 (2024); 16-21 1669-6840 1668-4834 spa https://revistas.unne.edu.ar/index.php/vet/article/view/7858/7380 https://creativecommons.org/licenses/by-nc/4.0
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-154
container_title_str Revistas UNNE - Universidad Nacional del Noroeste (UNNE)
language Español
format Artículo revista
topic Laboratory animals
Rat embryos
Sample size
Statistical analysis
Animales de laboratorio
Embriones de rata
Cálculo de muestra
Análisis estadístico
spellingShingle Laboratory animals
Rat embryos
Sample size
Statistical analysis
Animales de laboratorio
Embriones de rata
Cálculo de muestra
Análisis estadístico
Denis, B.
De Jesus, R.
Gittens, R.A
Estimating attrition of Sprague Dawley rats for research involving primary neuronal cultures
topic_facet Laboratory animals
Rat embryos
Sample size
Statistical analysis
Animales de laboratorio
Embriones de rata
Cálculo de muestra
Análisis estadístico
author Denis, B.
De Jesus, R.
Gittens, R.A
author_facet Denis, B.
De Jesus, R.
Gittens, R.A
author_sort Denis, B.
title Estimating attrition of Sprague Dawley rats for research involving primary neuronal cultures
title_short Estimating attrition of Sprague Dawley rats for research involving primary neuronal cultures
title_full Estimating attrition of Sprague Dawley rats for research involving primary neuronal cultures
title_fullStr Estimating attrition of Sprague Dawley rats for research involving primary neuronal cultures
title_full_unstemmed Estimating attrition of Sprague Dawley rats for research involving primary neuronal cultures
title_sort estimating attrition of sprague dawley rats for research involving primary neuronal cultures
description The use of laboratory animals for biomedical research is strictly regulated to prevent their suffering and inadequate use. While experimentation is in process, attrition usually can increase the number of animals needed to achieve the objectives. Unfortunately, attrition is not commonly reported in literature. In the present study, we worked with primary neuronal cell cultures to establish the first in vitro brain ischemia model in Panama. We report the effects of attrition in the final number of animals. Primary cortical cell cultures were produced from Sprague Dawley rat embryos. Vaginal cytology was used to evaluate oestrus cycle. Pregnant rats had routine evaluation plus clinical/weight follow up. Sample size calculations estimated 26 rats for the study using an attrition rate of 25%. The final number of animals used empirically was 26; however, the measured attrition was 50% (13). These results highlight the importance of calculating attrition for studies requiring laboratory animals, taking in consideration the context and available data from local institutions to allow for the most efficient use of laboratory animals, especially for newly established laboratories in developing countries.
publisher Universidad Nacional del Nordeste
publishDate 2024
url https://revistas.unne.edu.ar/index.php/vet/article/view/7858
work_keys_str_mv AT denisb estimatingattritionofspraguedawleyratsforresearchinvolvingprimaryneuronalcultures
AT dejesusr estimatingattritionofspraguedawleyratsforresearchinvolvingprimaryneuronalcultures
AT gittensra estimatingattritionofspraguedawleyratsforresearchinvolvingprimaryneuronalcultures
AT denisb estimaciondeincidenciasenratasspraguedawleyeninvestigacionesqueinvolucrencultivosneuronalesprimarios
AT dejesusr estimaciondeincidenciasenratasspraguedawleyeninvestigacionesqueinvolucrencultivosneuronalesprimarios
AT gittensra estimaciondeincidenciasenratasspraguedawleyeninvestigacionesqueinvolucrencultivosneuronalesprimarios
first_indexed 2025-05-17T05:10:30Z
last_indexed 2025-05-17T05:10:30Z
_version_ 1832343181108183040

Ejemplares similares