Effect of parenteral trace element supplementation on oxidative stress and transcriptomic profile of peripheral blood in peripartum dairy cows
The transition period is the most critical period in the lactation cycle of dairy cattle. During this period, cows can be exposed to high oxidative stress (OS), which can be managed through mineral supplementation with antioxidant micronutrients. The aim of this study was to evaluate the gene expres...
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Universidad Nacional del Nordeste
2024
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| Materias: | |
| Acceso en línea: | https://revistas.unne.edu.ar/index.php/vet/article/view/7857 |
| Aporte de: |
| id |
I48-R154-article-7857 |
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ojs |
| institution |
Universidad Nacional del Nordeste |
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I-48 |
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R-154 |
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Revistas UNNE - Universidad Nacional del Noroeste (UNNE) |
| language |
Español |
| format |
Artículo revista |
| topic |
Dairy cows Oxidative stress Transcriptome Transition period Mineral supplementation Ganado lechero Estrés oxidativo Transcriptoma Período de transición Suplementación mineral |
| spellingShingle |
Dairy cows Oxidative stress Transcriptome Transition period Mineral supplementation Ganado lechero Estrés oxidativo Transcriptoma Período de transición Suplementación mineral Calcaterra, F. Fernández, M.E. Olivera, L.H Guzmán Loza, A. Giovambattista, G. Picco, S.J. Effect of parenteral trace element supplementation on oxidative stress and transcriptomic profile of peripheral blood in peripartum dairy cows |
| topic_facet |
Dairy cows Oxidative stress Transcriptome Transition period Mineral supplementation Ganado lechero Estrés oxidativo Transcriptoma Período de transición Suplementación mineral |
| author |
Calcaterra, F. Fernández, M.E. Olivera, L.H Guzmán Loza, A. Giovambattista, G. Picco, S.J. |
| author_facet |
Calcaterra, F. Fernández, M.E. Olivera, L.H Guzmán Loza, A. Giovambattista, G. Picco, S.J. |
| author_sort |
Calcaterra, F. |
| title |
Effect of parenteral trace element supplementation on oxidative stress and transcriptomic profile of peripheral blood in peripartum dairy cows |
| title_short |
Effect of parenteral trace element supplementation on oxidative stress and transcriptomic profile of peripheral blood in peripartum dairy cows |
| title_full |
Effect of parenteral trace element supplementation on oxidative stress and transcriptomic profile of peripheral blood in peripartum dairy cows |
| title_fullStr |
Effect of parenteral trace element supplementation on oxidative stress and transcriptomic profile of peripheral blood in peripartum dairy cows |
| title_full_unstemmed |
Effect of parenteral trace element supplementation on oxidative stress and transcriptomic profile of peripheral blood in peripartum dairy cows |
| title_sort |
effect of parenteral trace element supplementation on oxidative stress and transcriptomic profile of peripheral blood in peripartum dairy cows |
| description |
The transition period is the most critical period in the lactation cycle of dairy cattle. During this period, cows can be exposed to high oxidative stress (OS), which can be managed through mineral supplementation with antioxidant micronutrients. The aim of this study was to evaluate the gene expression profiles of transition dairy cows supplemented with the antioxidant trace elements copper (Cu), zinc (Zn), manganese (Mn) and selenium (Se). The study was conducted in a commercial Holstein dairy farm located in General Belgrano, Buenos Aires, Argentina. Cows (n = 12) were randomly assigned to either a supplemented or a control group (n = 6 each). Blood samples were obtained on the day of calving and 4, 7 and 14 days after calving. Superoxide dismutase and glutathione peroxidase activity, antioxidant capacity (AC), and thiobarbituric acid reactive substances were determined. With these data, OS index was calculated. Additionally, RNA-sequencing analysis was performed on day +7, when OS index differences between groups were highest. The OS index and AC differed significantly between groups (day +7), despite only two differentially expressed genes codified for second messengers (p ≤ 0.05). This would suggest that trace mineral supplementation in transition dairy cows does not induce changes in the gene expression profiles of pathways associated with OS and immune function, whose expression is already high in response to the high OS levels and the dietary changes during this period. Nevertheless, supplementation with the studied minerals improved the pro-oxidant/ anti-oxidant balance. |
| publisher |
Universidad Nacional del Nordeste |
| publishDate |
2024 |
| url |
https://revistas.unne.edu.ar/index.php/vet/article/view/7857 |
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2025-05-17T05:10:30Z |
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I48-R154-article-78572024-11-01T13:19:27Z Effect of parenteral trace element supplementation on oxidative stress and transcriptomic profile of peripheral blood in peripartum dairy cows Análisis de la transcriptómica y del estado oxidativo en sangre de vacas lecheras suplementadas con minerales durante el período de transición Calcaterra, F. Fernández, M.E. Olivera, L.H Guzmán Loza, A. Giovambattista, G. Picco, S.J. Dairy cows Oxidative stress Transcriptome Transition period Mineral supplementation Ganado lechero Estrés oxidativo Transcriptoma Período de transición Suplementación mineral The transition period is the most critical period in the lactation cycle of dairy cattle. During this period, cows can be exposed to high oxidative stress (OS), which can be managed through mineral supplementation with antioxidant micronutrients. The aim of this study was to evaluate the gene expression profiles of transition dairy cows supplemented with the antioxidant trace elements copper (Cu), zinc (Zn), manganese (Mn) and selenium (Se). The study was conducted in a commercial Holstein dairy farm located in General Belgrano, Buenos Aires, Argentina. Cows (n = 12) were randomly assigned to either a supplemented or a control group (n = 6 each). Blood samples were obtained on the day of calving and 4, 7 and 14 days after calving. Superoxide dismutase and glutathione peroxidase activity, antioxidant capacity (AC), and thiobarbituric acid reactive substances were determined. With these data, OS index was calculated. Additionally, RNA-sequencing analysis was performed on day +7, when OS index differences between groups were highest. The OS index and AC differed significantly between groups (day +7), despite only two differentially expressed genes codified for second messengers (p ≤ 0.05). This would suggest that trace mineral supplementation in transition dairy cows does not induce changes in the gene expression profiles of pathways associated with OS and immune function, whose expression is already high in response to the high OS levels and the dietary changes during this period. Nevertheless, supplementation with the studied minerals improved the pro-oxidant/ anti-oxidant balance. El periodo de transición es la etapa más crítica en la lactancia de vacas lecheras, durante el cual los animales pueden estar expuestas a altos niveles de estrés oxidativo (EO), debido a sus funciones, la suplementación con minerales antioxidantes podría colaborar a su control. El objetivo de este trabajo consistió en evaluar los perfiles de expresión génica de vacas lecheras en el periparto suplementadas con los minerales antioxidantes cobre, zinc, manganeso y selenio. El estudio se llevó a cabo en un tambo comercial en la localidad de General Belgrano (Buenos Aires, Argentina). Los animales (n=12) fueron asignados al azar al grupo suplementado o al control (ambos con n=6). Las muestras de sangre se obtuvieron el día del parto (día 0) y en los días 4, 7 y 14 posteriores. Se midió la actividad de las enzimas superóxido dismutasa y glutatión peroxidasa, la capacidad antioxidante y las sustancias reactivas al ácido tiobarbitúrico. Con esta información se estimó el índice EO y también se realizó la transcriptómica mediante secuenciación de nueva generación (NGS) en el día +7, cuando la diferencia en el índice de EO entre ambos grupos era significativa. Del total de genes expresados, sólo hubo dos genes diferencialmente expresados, que codifican para segundos mensajeros. Esto sugiere que la suplementación con minerales antioxidantes de vacas lecheras en el periparto no induciría un aumento en la expresión de genes asociados cuya expresión ya se encuentra elevada debido a los altos niveles de EO y los cambios en la dieta asociados a este periodo. Sin embargo, la suplementación de estos minerales si mejoró el balance prooxidante / antioxidante. Universidad Nacional del Nordeste 2024-11-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/vet/article/view/7857 10.30972/vet.3527857 Revista Veterinaria; Vol. 35 Núm. 2 (2024); 8-15 1669-6840 1668-4834 spa https://revistas.unne.edu.ar/index.php/vet/article/view/7857/7379 https://creativecommons.org/licenses/by-nc/4.0 |