Effect of two sperm capacitation media (B.O vs Percoll) on in vitro fertilization of bovine oocytes

The study aimed to evaluate the Fertilization Rate of four independent in vitro treatments of bovine oocytes with Sexed (SX) and Conventional (Conv) semen, capacitated (B.O and Percoll) using the Swim-Up technique. Cleavage was assessed at 48 hours post-fertilization and on the seventh day of embryo...

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Autores principales: Tinoco, L., Capa Mora, E. D., Carrera, R.
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional del Nordeste 2024
Materias:
capacitation
motility
embryonic development
in vitro fertilization
percoll
capacitación
motilidad
desarrollo embrionario
fertilización in vitro
Acceso en línea:https://revistas.unne.edu.ar/index.php/vet/article/view/7479
Aporte de:
Revistas UNNE - Universidad Nacional del Noroeste (UNNE) de Universidad Nacional del Nordeste
id I48-R154-article-7479
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spelling I48-R154-article-74792024-05-15T00:22:45Z Effect of two sperm capacitation media (B.O vs Percoll) on in vitro fertilization of bovine oocytes Efecto de dos medios de capacitación espermática (B.O vs Percoll) en la fecundación in vitro de ovocitos bovinos Tinoco, L. Capa Mora, E. D. Carrera, R. capacitation motility embryonic development in vitro fertilization percoll capacitación motilidad desarrollo embrionario fertilización in vitro The study aimed to evaluate the Fertilization Rate of four independent in vitro treatments of bovine oocytes with Sexed (SX) and Conventional (Conv) semen, capacitated (B.O and Percoll) using the Swim-Up technique. Cleavage was assessed at 48 hours post-fertilization and on the seventh day of embryonic development. The results showed a cleavage percentage of 59.4% in T1 (Conv. Percoll), 60% in T2 (Conv. B.O), 75% in T3 (SX. Percoll), and 46.6% in T4 (SX. B.O), with a statistically significant difference (p>0.05) observed specifically between T3 and T4. The embryo development rate on the seventh day was 34.4% in T1, 20% in T2, 15% in T3, and 10.51% in T4. In this case, a statistically significant difference was found in the use of conventional medium with Percoll compared to SX B.O medium (p<0.05) (T1 and T4, respectively). The results also showed similar production of morula and early blastocysts and protruded embryos among the four treatments, demonstrating the feasibility of in vitro fertilization (IVF) technique for producing bovine embryos. In conclusion, the preliminary evaluation highlights the significant influence of the sexing method and treatment on semen quality and embryonic development in animal reproduction programs. Although Sexed semen with Percoll shows potential to improve cleavage rates, conventional semen, especially in T1 treatment (Conv. Percoll), exhibits superiority in individual motility and concentration. These findings provide crucial insights for optimizing reproductive programs. El objetivo del presente estudio fue evaluar la Tasa de Fecundación in vitro de ovocitos bovinos con semen sexado (SX) y convencional (Conv.) capacitados (B.O y Percoll) con la técnica Swim-Up. Se evaluó el clivaje a las 48 horas post fecundación y al séptimo día del desarrollo embrionario. Los resultados mostraron un porcentaje de clivaje del 59,4% en el T1 (Conv. Percoll), 60% en el T2 (Conv. B.O), 75% en el T3 (SX. Percoll) y 46,6% en el T4 (SX. B.O), mostrando diferencia estadística entre T3 y T4 (p>0,05). La tasa de desarrollo del embrión al séptimo día fue 34,4% T1, 20% en T2, 15% en T3 y 10,51% en T4; en este caso, se encontró diferencia estadísticamente significativa en el uso del medio convencional más Percoll en comparación con el medio de SX B.O (p<0,05) (T1 y T4, respectivamente). Los resultados también mostraron una producción similar de mórulas y blastocitos tempranos y protruidos entre los cuatro tratamientos, y demuestran la viabilidad del desarrollo e implementación de la técnica de fertilización in vitro (FIV) para producir embriones bovinos. Concluyendo la evaluación preliminar, destaca la significativa influencia del método de sexado y del tratamiento en la calidad del semen y desarrollo embrionario en programas de reproducción animal. Aunque el semen sexado con Percoll muestra potencial para mejorar tasas de clivaje, el semen convencional, especialmente en el tratamiento T1 (Conv. Percoll), exhibe una superioridad en motilidad individual y concentración. Estos resultados informan decisiones cruciales para optimizar programas reproductivos. Universidad Nacional del Nordeste 2024-05-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/vet/article/view/7479 10.30972/vet.3517479 Revista Veterinaria; Vol. 35 Núm. 1 (2024); 48-55 1669-6840 1668-4834 spa https://revistas.unne.edu.ar/index.php/vet/article/view/7479/6911 https://creativecommons.org/licenses/by-nc/4.0
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-154
container_title_str Revistas UNNE - Universidad Nacional del Noroeste (UNNE)
language Español
format Artículo revista
topic capacitation
motility
embryonic development
in vitro fertilization
percoll
capacitación
motilidad
desarrollo embrionario
fertilización in vitro
spellingShingle capacitation
motility
embryonic development
in vitro fertilization
percoll
capacitación
motilidad
desarrollo embrionario
fertilización in vitro
Tinoco, L.
Capa Mora, E. D.
Carrera, R.
Effect of two sperm capacitation media (B.O vs Percoll) on in vitro fertilization of bovine oocytes
topic_facet capacitation
motility
embryonic development
in vitro fertilization
percoll
capacitación
motilidad
desarrollo embrionario
fertilización in vitro
author Tinoco, L.
Capa Mora, E. D.
Carrera, R.
author_facet Tinoco, L.
Capa Mora, E. D.
Carrera, R.
author_sort Tinoco, L.
title Effect of two sperm capacitation media (B.O vs Percoll) on in vitro fertilization of bovine oocytes
title_short Effect of two sperm capacitation media (B.O vs Percoll) on in vitro fertilization of bovine oocytes
title_full Effect of two sperm capacitation media (B.O vs Percoll) on in vitro fertilization of bovine oocytes
title_fullStr Effect of two sperm capacitation media (B.O vs Percoll) on in vitro fertilization of bovine oocytes
title_full_unstemmed Effect of two sperm capacitation media (B.O vs Percoll) on in vitro fertilization of bovine oocytes
title_sort effect of two sperm capacitation media (b.o vs percoll) on in vitro fertilization of bovine oocytes
description The study aimed to evaluate the Fertilization Rate of four independent in vitro treatments of bovine oocytes with Sexed (SX) and Conventional (Conv) semen, capacitated (B.O and Percoll) using the Swim-Up technique. Cleavage was assessed at 48 hours post-fertilization and on the seventh day of embryonic development. The results showed a cleavage percentage of 59.4% in T1 (Conv. Percoll), 60% in T2 (Conv. B.O), 75% in T3 (SX. Percoll), and 46.6% in T4 (SX. B.O), with a statistically significant difference (p>0.05) observed specifically between T3 and T4. The embryo development rate on the seventh day was 34.4% in T1, 20% in T2, 15% in T3, and 10.51% in T4. In this case, a statistically significant difference was found in the use of conventional medium with Percoll compared to SX B.O medium (p<0.05) (T1 and T4, respectively). The results also showed similar production of morula and early blastocysts and protruded embryos among the four treatments, demonstrating the feasibility of in vitro fertilization (IVF) technique for producing bovine embryos. In conclusion, the preliminary evaluation highlights the significant influence of the sexing method and treatment on semen quality and embryonic development in animal reproduction programs. Although Sexed semen with Percoll shows potential to improve cleavage rates, conventional semen, especially in T1 treatment (Conv. Percoll), exhibits superiority in individual motility and concentration. These findings provide crucial insights for optimizing reproductive programs.
publisher Universidad Nacional del Nordeste
publishDate 2024
url https://revistas.unne.edu.ar/index.php/vet/article/view/7479
work_keys_str_mv AT tinocol effectoftwospermcapacitationmediabovspercolloninvitrofertilizationofbovineoocytes
AT capamoraed effectoftwospermcapacitationmediabovspercolloninvitrofertilizationofbovineoocytes
AT carrerar effectoftwospermcapacitationmediabovspercolloninvitrofertilizationofbovineoocytes
AT tinocol efectodedosmediosdecapacitacionespermaticabovspercollenlafecundacioninvitrodeovocitosbovinos
AT capamoraed efectodedosmediosdecapacitacionespermaticabovspercollenlafecundacioninvitrodeovocitosbovinos
AT carrerar efectodedosmediosdecapacitacionespermaticabovspercollenlafecundacioninvitrodeovocitosbovinos
first_indexed 2024-08-21T22:35:57Z
last_indexed 2024-08-21T22:35:57Z
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