Usefulness of nested PCR in the diagnosis of canine leishmaniosis in Corrientes
Leishmaniosis is a disease caused by protozoan parasites of the genus Leishmania and, in the New World, is transmitted to mammals through the bite of blood-sucking dipteran insects of the genus Lutzomyia. Canines are susceptible to infection, developing the visceral form (VL) and the cutaneous form...
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Universidad Nacional del Nordeste
2024
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| Acceso en línea: | https://revistas.unne.edu.ar/index.php/vet/article/view/7471 |
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I48-R154-article-74712024-05-15T00:22:45Z Usefulness of nested PCR in the diagnosis of canine leishmaniosis in Corrientes Utilidad de PCR anidada en el diagnóstico de leishmaniosis canina en Corrientes Jastrzebsky, F. A. Vega, L. E. Maruñak, S. L. Esquivel, G. P. Montesi, A. M. Almirón, E. C. Cabrera, W. R. De Biasio, M. B. canine Lutzomyia molecular biology caninos Lutzomyia biología molecular Leishmaniosis is a disease caused by protozoan parasites of the genus Leishmania and, in the New World, is transmitted to mammals through the bite of blood-sucking dipteran insects of the genus Lutzomyia. Canines are susceptible to infection, developing the visceral form (VL) and the cutaneous form (CL) of the disease. Diagnostic methods include direct observation of the parasite by light microscopy, serology, or molecular biology techniques in samples from dogs with clinical signs and/or clinical suspicion. In this word, the usefulness of a nested PCR designed for the diagnosis of this pathology in dogs was evaluated. A total of 18 samples of EDTA pre-treated dog’s whole blood were analyzed. Dogs were from the city of Corrientes and presented clinical suspicion of suffering from leishmaniasis. From the samples, deoxyribonucleic acid (DNA) was extracted and two successive rounds of amplification of a sequence that codes for the small subunit of ribosomal RNA were carried out. The results obtained from the nested PCRs were compared with this obtained from the direct parasitological method (gold standard). The applied technique showed a sensitivity of 100%, specificity of 75%, positive predictive value of 67% and negative predictive value of 100%, turning it into a valuable tool for diagnosing canine leishmaniosis. La leishmaniosis es una enfermedad causada por parásitos protozoarios del género Leishmania y transmitida a mamíferos mediante la picadura de insectos dípteros hematófagos del género Lutzomyia en el nuevo mundo. Los caninos son susceptibles de adquirir la forma visceral y la forma cutánea de la enfermedad. Los métodos de diagnóstico incluyen observación directa del parásito por microscopía óptica, serología o técnicas de biología molecular en muestras de perros con signos clínicos evidentes y/o sospecha clínica. En este estudio se evaluó la utilidad de una reacción en cadena de la polimerasa anidada (PCRn) para el diagnóstico de dicha patología en perros. Se analizaron 18 muestras de sangre entera anticoagulada con EDTA de perros de la ciudad de Corrientes con sospecha clínica de padecer leishmaniosis. A partir de las mismas, se extrajo ácido desoxiribonucleico (ADN) y se realizaron dos rondas de amplificación sucesivas de una secuencia que codifica para la subunidad pequeña de ARN ribosomal. Los resultados obtenidos de las PCR anidadas fueron comparados con los resultados obtenidos a partir del método parasitológico directo considerado como gold standard. La técnica aplicada mostró sensibilidad del 100%, especificidad del 75%, valor predictivo positivo del 67% y valor predictivo negativo del 100% lo que la convierte en una herramienta útil para el diagnóstico de leishmaniosis canina. Universidad Nacional del Nordeste 2024-05-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/vet/article/view/7471 10.30972/vet.3517471 Revista Veterinaria; Vol. 35 Núm. 1 (2024); 1-7 1669-6840 1668-4834 spa https://revistas.unne.edu.ar/index.php/vet/article/view/7471/6903 https://creativecommons.org/licenses/by-nc/4.0 |
| institution |
Universidad Nacional del Nordeste |
| institution_str |
I-48 |
| repository_str |
R-154 |
| container_title_str |
Revistas UNNE - Universidad Nacional del Noroeste (UNNE) |
| language |
Español |
| format |
Artículo revista |
| topic |
canine Lutzomyia molecular biology caninos Lutzomyia biología molecular |
| spellingShingle |
canine Lutzomyia molecular biology caninos Lutzomyia biología molecular Jastrzebsky, F. A. Vega, L. E. Maruñak, S. L. Esquivel, G. P. Montesi, A. M. Almirón, E. C. Cabrera, W. R. De Biasio, M. B. Usefulness of nested PCR in the diagnosis of canine leishmaniosis in Corrientes |
| topic_facet |
canine Lutzomyia molecular biology caninos Lutzomyia biología molecular |
| author |
Jastrzebsky, F. A. Vega, L. E. Maruñak, S. L. Esquivel, G. P. Montesi, A. M. Almirón, E. C. Cabrera, W. R. De Biasio, M. B. |
| author_facet |
Jastrzebsky, F. A. Vega, L. E. Maruñak, S. L. Esquivel, G. P. Montesi, A. M. Almirón, E. C. Cabrera, W. R. De Biasio, M. B. |
| author_sort |
Jastrzebsky, F. A. |
| title |
Usefulness of nested PCR in the diagnosis of canine leishmaniosis in Corrientes |
| title_short |
Usefulness of nested PCR in the diagnosis of canine leishmaniosis in Corrientes |
| title_full |
Usefulness of nested PCR in the diagnosis of canine leishmaniosis in Corrientes |
| title_fullStr |
Usefulness of nested PCR in the diagnosis of canine leishmaniosis in Corrientes |
| title_full_unstemmed |
Usefulness of nested PCR in the diagnosis of canine leishmaniosis in Corrientes |
| title_sort |
usefulness of nested pcr in the diagnosis of canine leishmaniosis in corrientes |
| description |
Leishmaniosis is a disease caused by protozoan parasites of the genus Leishmania and, in the New World, is transmitted to mammals through the bite of blood-sucking dipteran insects of the genus Lutzomyia. Canines are susceptible to infection, developing the visceral form (VL) and the cutaneous form (CL) of the disease. Diagnostic methods include direct observation of the parasite by light microscopy, serology, or molecular biology techniques in samples from dogs with clinical signs and/or clinical suspicion. In this word, the usefulness of a nested PCR designed for the diagnosis of this pathology in dogs was evaluated. A total of 18 samples of EDTA pre-treated dog’s whole blood were analyzed. Dogs were from the city of Corrientes and presented clinical suspicion of suffering from leishmaniasis. From the samples, deoxyribonucleic acid (DNA) was extracted and two successive rounds of amplification of a sequence that codes for the small subunit of ribosomal RNA were carried out. The results obtained from the nested PCRs were compared with this obtained from the direct parasitological method (gold standard). The applied technique showed a sensitivity of 100%, specificity of 75%, positive predictive value of 67% and negative predictive value of 100%, turning it into a valuable tool for diagnosing canine leishmaniosis. |
| publisher |
Universidad Nacional del Nordeste |
| publishDate |
2024 |
| url |
https://revistas.unne.edu.ar/index.php/vet/article/view/7471 |
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