Kinetic of the uptake of organic substrates of cateria isolated from Chascomús Pond

Eight bacterial strains were isolated from Chascomús Pond (Buenos Aires Province) and their capacity to growth at low concentrations of glycolate and glucose, as a solé source of organic carbón was evaluated.Substrate affinities (Kt valúes) and other kinetic parameters were measured by Wright &a...

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Autores principales: Romero, María C., Mariazzi, Aldo A.
Formato: Artículo revista
Lenguaje:Español
Publicado: Centro de Ecología Aplicada del Litoral (Consejo Nacional de Investigaciones Científicas y Técnicas - Universidad Nacional de Nordeste) 2023
Acceso en línea:https://revistas.unne.edu.ar/index.php/eco/article/view/7071
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Revistas UNNE - Universidad Nacional del Noroeste (UNNE) de Universidad Nacional del Nordeste
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spelling I48-R154-article-70712023-12-01T20:20:03Z Kinetic of the uptake of organic substrates of cateria isolated from Chascomús Pond Cinética de consumo de sustratos orgánicos de bacterias aisladas de la Laguna de Chascomus Romero, María C. Mariazzi, Aldo A. Eight bacterial strains were isolated from Chascomús Pond (Buenos Aires Province) and their capacity to growth at low concentrations of glycolate and glucose, as a solé source of organic carbón was evaluated.Substrate affinities (Kt valúes) and other kinetic parameters were measured by Wright & Hobbie methodology with 14C-labelled glycollate and MC-labelled glucose as substrates. Four o f the eight puré culture studied followed Michaelis-Menten uptakekinetics giving good straight lines after the Lineweaver-Burke transfonnation.Vmax valúes o f natural popuiations and the experimental ones were similar for glycollate; for glucose the experimental valúes were a little higher. This suggest the possibility o f applying the experimental Kt valúes to natural popuiations as a way of evaluation o f the natural substrate concentration. Eight bacterial strains were isolated from Chascomús Pond (Buenos Aires Province) and their capacity to growth at low concentrations of glycolate and glucose, as a solé source of organic carbón was evaluated.Substrate affinities (Kt valúes) and other kinetic parameters were measured by Wright & Hobbie methodology with 14C-labelled glycollate and MC-labelled glucose as substrates. Four o f the eight puré culture studied followed Michaelis-Menten uptakekinetics giving good straight lines after the Lineweaver-Burke transfonnation.Vmax valúes o f natural popuiations and the experimental ones were similar for glycollate; for glucose the experimental valúes were a little higher. This suggest the possibility o f applying the experimental Kt valúes to natural popuiations as a way of evaluation o f the natural substrate concentration. Centro de Ecología Aplicada del Litoral (Consejo Nacional de Investigaciones Científicas y Técnicas - Universidad Nacional de Nordeste) 2023-11-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artículo revisado por pares application/pdf https://revistas.unne.edu.ar/index.php/eco/article/view/7071 10.30972/eco.16277071 Ecosur; 1990: vol. 16, no. 27; 31-43 0325-108X spa https://revistas.unne.edu.ar/index.php/eco/article/view/7071/6552
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-154
container_title_str Revistas UNNE - Universidad Nacional del Noroeste (UNNE)
language Español
format Artículo revista
author Romero, María C.
Mariazzi, Aldo A.
spellingShingle Romero, María C.
Mariazzi, Aldo A.
Kinetic of the uptake of organic substrates of cateria isolated from Chascomús Pond
author_facet Romero, María C.
Mariazzi, Aldo A.
author_sort Romero, María C.
title Kinetic of the uptake of organic substrates of cateria isolated from Chascomús Pond
title_short Kinetic of the uptake of organic substrates of cateria isolated from Chascomús Pond
title_full Kinetic of the uptake of organic substrates of cateria isolated from Chascomús Pond
title_fullStr Kinetic of the uptake of organic substrates of cateria isolated from Chascomús Pond
title_full_unstemmed Kinetic of the uptake of organic substrates of cateria isolated from Chascomús Pond
title_sort kinetic of the uptake of organic substrates of cateria isolated from chascomús pond
description Eight bacterial strains were isolated from Chascomús Pond (Buenos Aires Province) and their capacity to growth at low concentrations of glycolate and glucose, as a solé source of organic carbón was evaluated.Substrate affinities (Kt valúes) and other kinetic parameters were measured by Wright & Hobbie methodology with 14C-labelled glycollate and MC-labelled glucose as substrates. Four o f the eight puré culture studied followed Michaelis-Menten uptakekinetics giving good straight lines after the Lineweaver-Burke transfonnation.Vmax valúes o f natural popuiations and the experimental ones were similar for glycollate; for glucose the experimental valúes were a little higher. This suggest the possibility o f applying the experimental Kt valúes to natural popuiations as a way of evaluation o f the natural substrate concentration.
publisher Centro de Ecología Aplicada del Litoral (Consejo Nacional de Investigaciones Científicas y Técnicas - Universidad Nacional de Nordeste)
publishDate 2023
url https://revistas.unne.edu.ar/index.php/eco/article/view/7071
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AT mariazzialdoa kineticoftheuptakeoforganicsubstratesofcateriaisolatedfromchascomuspond
AT romeromariac cineticadeconsumodesustratosorganicosdebacteriasaisladasdelalagunadechascomus
AT mariazzialdoa cineticadeconsumodesustratosorganicosdebacteriasaisladasdelalagunadechascomus
first_indexed 2024-08-21T22:38:49Z
last_indexed 2024-08-21T22:38:49Z
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